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Objective To study the inhibitive effect of prion protein on the formation of microtubules enhanced by microtubule-associated protein tau.

目的研究朊蛋白对微管相关蛋白介导的体外微管形成的影响。

The differences of providing amino acid to kernel of different winter wheatculfivars.93-52 with high protein content in kernel had highest content of amino acid innutrition organs,indicated 93-52 can provide abundance material for protein synthesis ofkernel,namely the source in full,so the content of amino acid in kernel was highest;Compared Lumai21 with Jinanl7,the amino acid content in leaf of Lumai21 was a littlehigher than Jinan17,but the amino acid content in stem、sheath and spike was obviouslylower than Jinan17,also the amount of amino acid restored in nutrition organs was large,the translocation degree was low to kernel,namely the flow was not fluency,which wasthe main reason of low content of amino acid in kernel.

小麦不同品种向籽粒供应氨基酸水平的差异。籽粒蛋白质含量高的93-52各营养器官中的游离氨基酸含量均最高,说明93-52籽粒蛋白质合成所需底物供应充足,即源足,因而其籽粒游离氨基酸含量最高。鲁麦21和济南17相比,鲁麦21叶片中的游离氨基酸含量虽略高于济南17,但茎鞘和穗中的游离氨基酸含量却明显比济南17低,并且在后期鲁麦21营养器官中游离氨基酸滞留的多,向籽粒中的转移程度低,即流不畅,是造成鲁麦21籽粒游离氨基酸含量低的主要原因之一。

Aim To investigate the effect of protein kinase C inhibitor on expression of intercellular adhesion molecule- 1 protein and mRNA in smoking rats.

目的探讨蛋白激酶C抑制剂对吸烟大鼠脑血管内皮细胞细胞间粘附分子1蛋白和mRNA表达的影响。

Results (1)LVMI gradually increased from 5d to 30d in OG (P<0.01);(2)Counts of intracardiac bFGF immune reaction and bFGF mRNA expression were increased at 5d, were essentially normalized at 30d;(3) Intracardial ACE activity, angiotensin II concentration, ACE protein and ACE mRNA expression were progressively risen from 0.5 h or 4 h to 30 d;(4)LVMI was positively correlated with the level of ACE protein and ACE mRNA expression following 5d.

2OG中心肌bFGF免疫反应特异性染色强度增加,半定量显示5天升高(P<0.05),30天基本恢复正常(P>0.05);bFGF mRNA表达1天升高(P<0.05),其后类似bFGF。(3)心肌血管紧张素转换酶活性和血管紧张素Ⅱ水平在术后0.5小时即升高(P<0.05),4小时明显增高(P<0.01),峰值在30天;心肌ACE蛋白及ACE mRNA表达均在术后4小时明显增高(P<0.05或<0.01),其后逐渐升高,峰值在30天;5天及其后,LVMI与ACE蛋白及ACE mRNA表达数量呈正相关。

In this chapter, we present a brief summary of protein aggregation by the amyloid pathway based on what is known from other organ systems and describe quality control mechanisms that exist intracellularly to control protein misfolding and aggregation.

我们先根据已知的器官系统来总结蛋白通过淀粉体通路而聚集的过程,并描述存在于胞内的控制蛋白错误折叠和聚集的质量控制机制。

The effects of G-protein and protein kinase inhibitor on the behavioral responses of rats to intradermal injection of capasaicin.

G旦白和旦白激酶抑制剂对大鼠皮内注射capasaicin引起的行为反应的影响。

Of 21 HCC shown to have 249 codon mutation, 20 exhibited high intranuclear accumulation of p53 protein. p53 protein staining was negative in the surrounding noncancerous hepatic tissue.

启东肝细胞癌中,p53基因发生着249密码子的持续性、纯合型的高频度错义突变,它伴随着p53蛋白在癌细胞核内较普遍的高度积累。

The recombinant plasmid GST-STAT3-4 which is a truncation of STAT3 was transformed into E. coli JM109 and then induced to express a fussion protein by IPTG successfully. and we also renatured the intranuclear indusions of the fussion protein GST-STAT3-4

在实验的第二部分中我们成功的将STAT3的一个截断体STAT3-4的融合蛋白GST-STAT3-4在大肠杆菌中做了表达,并对以包涵体形式存在的STAT3-4蛋白进行了复性。

And adsorption of jack bean proteins onto the amphoteric resin was studied in this paper. The amphoteric resin was in innersalt form. The results of adsorption experiments show that when the proteins are adsorpted into the amphoteric resin in the neutral phosphate buffer solution, the diffusion coefficient of protein is near twofold in 0.4 mol/L NaCl phosphate buffer solution than that in solution without salt, and the adsorption capacity of protein also rises at the same ti...

通过盐浓度对吸附的动力学和热力学影响的研究,结果表明,在中性磷酸盐缓冲液中,两性树脂吸附刀豆蛋白,有盐( 0 。4mol/L Na Cl)的比无盐的扩散系数提高将近一半,且吸附量增加,其吸附等温线的效率在最初始部分高于无 Na Cl时的;随着盐浓度( 0~ 1 mol/L Na Cl)的提高,树脂对蛋白的吸附量也变大,盐浓度提高时树脂膨胀体积的增大与蛋白吸附量的增加呈线性关系,内盐型两性树脂对大分子蛋白质的吸附作用与小分子高价离子的相似,同样是通过内盐键破裂吸附。

The mortality of mice immunized with 56-47kDa fusion protein was lower than that of the mice immunized with another recombinant protein after challenge of Ot Karp.

用56一和47一kDa蛋白免疫血清分别对转染的细胞作间接免疫荧光染色,结果证明56一和47一kDa蛋白基因以及它们的融合基因均在相应的转染的细胞内表达了目的蛋白。

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