查询词典 protein-free

The protein of 14.3kDa was then analyzed by gel filtration in ion exchange chromatography, and the MALDI-TOF/TOF-MS search on the Internet. The results indicate that the protein was hemocyanin subunit.

然后用Sephadex G75凝胶过滤层析和DEAE-52离子交换层析纯化出该过敏原，再经MALDI-TOF/TOF-MS检测，利用Blastz在线分析。

Methods: Human colon cancer cell line DLD1 was treated with Bcl-XL siRNA, The protein level of Bcl-XL in the cells was determined by Western blot; the apoptotic ratio and survival condition were detected by flow cytometry assay and trypan blue-stained cell counting with hemocytometer, respectively. Results: The level of Bcl-XL protein in DLD1 cells was obviously down-regulated by Bcl-XL siRNA.

采用人结肠癌细胞株DLD1，利用人工合成的Bcl-XL靶向小分子干扰RNA转染DLD1细胞，通过Western blot法检测转染细胞Bcl-XL蛋白的表达，流式细胞仪检测处理后细胞的凋亡情况，锥虫蓝染色法计数存活细胞。

It was so concluded that silkworm hemopoietic organs were related to the formation of hemolymph proteins, and due to the decrease of hemocytes caused by functional disruption of hemopoietic organs, synthesis of protein in fat body was affected and protein components in hemolymph changed.

由此认为家蚕幼虫造血器官与血淋巴中的蛋白质成分有关，造血器官的机能障碍、血球的数量减少可影响脂肪体中蛋白质的合成，从而使存在于血淋巴中的蛋白质成分发生变化。

This method could increase molecular weight of the medicamentous protein and decrease the immunogenicity of the heterogenesis protein, thereby it could diminish glomerular filtration rate and clearance rate in vivo.

该方法增大了蛋白药物的分子量，降低了异源蛋白的免疫原性，从而可以减少肾小球滤过率和体内清除率，有效提高IL-2在体内的半衰期，具有非常广阔的生产以及临床应用前景。

HIRA proteins are characterized by 7 conserved WD repeats in their amino-terminus, which are predicted to assemble into a β-propeller structure known to mediate protein-protein interactions.

HIRA蛋白家族在N端的7个WD重复区，推测形成一个β螺旋结构，该β螺旋结构可能与蛋白质—蛋白质互作有关。

Natural Hirudin has the ability of protein, but it is simpler and less molecular-weight than protein.

天然水蛭素的，有能力的蛋白质，但它是简单和低分子量比蛋白质。

Results Compared to those before treatment the volume of the implanted keloid of Group D began to decrease since 14 days after treatment time-dependently (all P 《 0.05), and the volumes of the other 3 groups continued to increase and peaked on days 21, 14, or 7 respectively (all P 《 0.05).Microscopy showed infiltration of a larger quantity of histiocyte in the keloid tissue, and more obvious collagen disorganization and apoptosis of fibroblasts in Group D than in the other 3 groups.The protein expression of Bcl-2 was more remarkable and the protein expression of BAX was less remarkable in Group D than iu the other 3 groups.

结果 用药前和用药后7、14、21、28、35 d，D组瘢痕疙瘩组织块体积(mm3)分别为173±5、172±5、147±5、125±6、112±7和84±9，从用药后14 d开始明显缩小(均P＜0.05)；而其他3组瘢痕疙瘩组织块体积均明显增大，从用约后7 d开始各时点测得的体积均明显大于D组(均P＜0.05)。D组瘢痕疙瘩组织中有大量小鼠组织细胞浸润，胶原结构破坏和成纤维细胞凋亡明显重于其他3组，Bcl-2蛋门质表达明显弱于而Bax蛋白质表达明显强于其他3组。

The level of tumor necrosis factore alpha, interleukin-6 (IL-6) and interleukin-8 (IL-8) were evaluated using ELISA method. Western blotting was used to detect the protein expression of protein kinase B and lipid phosphatase PTEN. Immunoprecipitations and radioactivity of γ〓P incorporated into its specific substrate histon H〓B was utilized to determine the PKB activation. Phosphate concentration released from PTEN-specific substrat diC16PIP3 was assessed using green reagent method.

以细胞计数、噻唑蓝比色法测定细胞增殖能力，酶联免疫法检测培养上清液中细胞因子TNFα、IL-6、IL-8水平，Western免疫印记定量蛋白激酶B、蛋白磷酸酶PTEN表达水平，免疫沉淀、特异底物组蛋白H2Bγ〓P掺入量测定PKB活性，特异底物dic16PIP3脱磷酸绿色试剂法反应PTEN活性。

Purpose to study the relationships between the expressions of cyclin d1 protein, bcl-2 and fas protein and apoptosis and proliferation of r-s/h cells in hodgkins lymphoma.

目的：探讨cyclin d1蛋白与霍奇金淋巴瘤r-s/h细胞凋亡及增殖的关系。

To obtain the African horse sickness virus VP7 protein, which is used to preparing serological diagnostic reagent and constructing AHS new generation vaccine, the prokaryotic expression is used to express VP7 protein.

为获得非洲马瘟病毒VP7蛋白，以用于制备AHS血清学诊断试剂并为AHS新型疫苗的构建奠定基础，笔者采用原核表达系统表达VP7蛋白。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷