查询词典 protein-free

III For constructing the expression vector of a fusion protein and obtain a target protein with full identity on aa sequence of a natural 13- 1,3-1 ,4-glucanase, with the recombined plasmid DNA harbouring the target gene as template, the primers designed with restriction sites for both terminals and enterokinase recognition site, followed by PCR amplification, was induced to the target gene.

为构建融合蛋白表达载体和获得与天然蛋白质序列完全一致的目的蛋白，以含有目的基因的重组质粒DNA为模板，设计引物时加入两端酶切位点及肠激酶裂解位点，通过PCR扩增引入目的基因中，测序结果表明接头和读框正确。

After thermal induction, no specific recombinant protein band in SDS-PAGE was found, but G-CSF activity was detectable. Therefore, a new recombinant plasmid pBVHG2 expressing hG-CSF hybrid protein with additional 8 amino acids which could be cut off specifically with the help of mucosal enterokinase at the N terminus of hG-CSF was constructed.

含此质粒菌株虽然表达菌体裂解后可测得明显的生物学活性，但SDS-PAGE仍未见特异表达产物带；因此，再应用相同方法，在hG-CSF cDNA突变体5′端增加24核苷酸对的FLAG肽编码序列，构建了hG-CSF杂交蛋白（hG-CSF天然蛋白N末端增加8aa的FLAG肽，后者可由肠激肽酶切除）的表达质粒pBVHG2。

Methods: The expression of p16 protein and nm 23 protein were detected by an immunohistochemistory assay in 130 cases of tissue samples including 30 normal ovary, 50 ovarian benign epithelioma and 50 ovarian epithelial carcinoma.

免疫组化SP法检测30例正常卵巢，50例卵巢上皮性良性肿瘤及50例卵巢癌组织中P16蛋白和nm23蛋白，并分析其阳性表达与肿瘤的关系。

Methods: The expression of P16 protein and nm23 protein were detected by streptavidin peroxidase conjugated method in 78 cases of tissue samples including 12 normal ovary,14 ovarian benign epithelioma and 52 OEC.

免疫组化S P法检测12 例正常卵巢，14 例卵巢上皮性良性肿瘤及52 例卵巢癌组织中 P16 蛋白和nm 23 蛋白的表达。

The protein encoded by RBB1 contained two conserve domain ATP-synt-DE-N and AtpC from 3 to 83 and from 1 to 133. All above showed that the full length cDNA RBB1 encoded ATP synthase CF1 epsilon chain protein The plant resistant mechanism is very sophisticated and one of the mainly responses is the metabolic change.

植物的抗病机理非常复杂，其主要反应之一就是代谢上的变化，例如，在利用cDNA微阵列对水稻抗稻瘟病近等基因系的分析中，检测到了很多与光合作用和糖代谢功能有关的差异表达片段；在小麦中也筛选到一个植物中ATP酶具有高度同源性的cDNA序列可能与小麦抗白粉病相关。

The invention further includes pharmaceutical compositions comprising the fusion protein and methods of using the fusion protein and/or the pharmaceutical compositions, for example to stimulate erythropoiesis in subjects in need of therapy.

本发明进一步包括包含所述融合蛋白的药物组合物，和使用所述融合蛋白和/或所述药物组合物(例如在需要治疗的受试者中刺激红细胞生成)的方法。

Objective To examinate the expression of GFAP and S - 100 protein between cerebral infarction and diabetes combined cerebral infarction by immunohistochemistry method. To detect the expression of GFAP and S -100 protein in diabetes.

目的 通过免疫组化方法，检测单纯脑梗塞、糖尿病合并脑梗塞时GFAP及S-100蛋白的表达特点以及糖尿病对GFAP及S-100蛋白表达的影响。

Is the chimeric protein of human epidermal growth factor and PE40 which is recombinant protein of the translocated subunit and killing subunit of Pseudomonas exotoxin A.

EGF-PE4 0是由人表皮生长因子基因与绿脓杆菌外毒素 A的跨膜亚单位和毒力亚单位( PE4 0 )基因融合，在大肠杆菌内表达的重组毒素。

Making gfp fusion protein is a convenient method to mark the expression of interesting proteins, but it may interfere the expression of interesting proteins, since gfp is a quite big protein itself.

一方面，作为标记与目的蛋白融合可以方便的标示目的蛋白的表达情况，但gfp本身是个相当大的蛋白，会降低目的蛋白的表达；另一方面，进一步分离时需使用抗gfp的单克隆抗体进行免疫共沉淀，既不经济又不方便。

In order to study the dependence of several factors on the protein reduction of the soybean protein fibre, the factorial experiment design was used to optimize the pretreatment conditions.

以测得的坯布平均含氮量为基准，测定时间、温度、pH值及碱剂浓度四个因素对大豆蛋白纤维含氮量的影响，并通过正交试验确定最佳前处理工艺条件。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。