查询词典 protein-free

We have successfully attained the fulllength cDNA sequence of FRG4, bioinformatic analysis shows FRG4 gene has 99%homology with Homosapiens synapse associated protein 1 (SYAP1), consists of 9 exons and 8 introns and located in Xp22.2. None transmembrane domains but a BSD domain was discovered, and its encoding protein is probably a water-solubility protein.

结果表明cDNA文库基础上运用热启动PCR获得FRG4全长cDNA序列，生物信息学分析显示FRG4基因与人类的突触相关蛋白有99%同源性；定位在X染色体的短臂2区2带2亚带2次亚带；由9个外显子和8个内含子组成；无跨膜区段；有一结构域BSD；该基因编码蛋白可能为一水溶性蛋白。

The nuclear protein encoded by N gene is consist of 422 AA residues (47kDa), Each virion contains 1258 copies that can effectively protect viral RNA from being digested by nucleotidase. The N protein presents a group specificity, it exists in various types and subtypes of VS and plays a very important role in transcript copy. Due to its characteristics of high antigenicity, the N protein can stimulate the body producing nonneutralized antibody.

它可有效的保护病毒RNA免受各种核酸酶的消化。N基因编码的N蛋白呈群特异性，为许多型和亚型所共有，有高的抗原性，刺激机体产生非中和抗体，且在转录复制中担任了重要的角色。

The results showed that (1) COX-2 mRNA and protein existed in the mature male rat testis. COX-2 was localized in spermatocytes by immunohistochemistry.(2) COX-2 also existed in adult man testis. COX-2 protein was positively stained in spermatocytes and Leydig cells.(3) 2 weeks after administration of rofecoxib, the level of testosterone in the whole testis reached its lower values, being only 50% of control values. However, testosterone level recovered during 4 weeks. After such treatment, histologic examination of these testes showed atrophy of the seminiferous tubules and maturation arrest of spermatogenesis.(4) 2 weeks post-EDS, expression of COX-2 decreased significantly (P.005), in comparison with vehicle-treatment control. 4 weeks after treatment, a new generation of fetal like Leydig cells repopulated in the testicular interstitium resulting in COX-2 expression partially recovered. Although the expression of COX-2 mRNA and protein are enhanced at 8th week after using external testosterone, it wasn't significant higher than control group.

实验研究证明：(1)正常成年雄性大鼠睾丸组织中COX-2在mRNA和蛋白质水平均存在表达，免疫组织化学结果显示COX-2定位于曲细精管内的生精细胞；正常成年男性睾丸组织中同样存在COX-2表达，免疫组织化学结果显示COX-2定位于曲细精管内的生精细胞和Leydig细胞；(2)服用特异性COX-2酶抑制剂rofecoxib 2周后，实验组大鼠睾丸组织内睾酮的含量减少，为正常对照组的50％；持续用药4周后睾丸组织内睾酮浓度逐渐恢复至正常水平；COX-2酶活性降低后病理组织切片显示睾丸内曲细精管萎缩，生精紊乱，持续用药4周时影响最明显；(3)注射特异性Leydig细胞杀灭剂EDS 2周后，实验组大鼠睾丸组织内睾酮浓度降至极低水平时，COX-2的蛋白和基因表达水平也显著低于正常空白对照组（P.005）；使用EDS后第4周，睾丸组织中的睾酮浓度逐渐回升，同样组织中COX-2蛋白表达和mRNA表达水平也相应提高接近正常水平；使用EDS后4周开始给予外源性睾酮，6周和8周时COX-2表达水平的绝对值虽有提高，但与正常大鼠空白对照组比较并无显著性差异，说明外源性雄激素刺激睾丸合成COX-2的作用并不明显。

Methods Lewis rats were immunized with the protein extracted from electric organ of Narcine timilei, the clinical manifestations were observed every day, and repetitive nerve stimulation,single fiber electromyography and the levels of acetylcholine receptor antibody were detected on the 49th days post immunization. The content of the protein extracted from electric organ of Narcine timile was 35.4 mg/mL, and the protein could respond with AChRAb from the serum of seropositive myasthenia gravis patient.

参照徐氏法从丁氏双鳍电鳐的电器官提取AChR蛋白，并采用Folin-酚试剂法及酶联免疫吸附法检测提取蛋白的含量及活性；以提取的蛋白主动免疫Lewis大鼠，每天观察其临床表现，并于免疫后7周进行低频重复电刺激、单纤维肌电图及血清中AChRAb水平的检测。

Methods Lewis rats were immunized with the protein extracted from electric organ of Narcine timilei, the clinical manifestations were observed every day, and repetitive nerve stimulation, ingle fiber electromyography and the levels of acetylcholine receptor antibody were detected on the 49th days post immunization. The content of the protein extracted from electric organ of Narcine timile was 35.4 mg/mL, and the protein could respond with AChRAb from the serum of seropositive myasthenia gravis patient.

参照徐氏法从丁氏双鳍电鳐的电器官提取AChR蛋白，并采用Folin-酚试剂法及酶联免疫吸附法检测提取蛋白的含量及活性；以提取的蛋白主动免疫Lewis大鼠，每天观察其临床表现，并于免疫后7周进行低频重复电刺激、单纤维肌电图及血清中AChRAb水平的检测。

Methods Conjugated with protein precipitation with trichloroacetic acid, BCA method was used. According to the positive relationship of protein content with the 570 nm absorbance, protein content was calculated in powdered milk.

使用三氯乙酸沉淀蛋白质后，运用BCA法，在570nm波长处，分别测定标准蛋白质应用液与样品稀释液的吸光度值，基于测定液中蛋白质含量与其吸光度值呈正比关系，计算出样品中蛋白质的含量。

With an increase in Bcl-2 protein, more Bax homodimers were separated and conjugated with Bcl-2 protein to form more stable Bcl-2/Bax heterodimers than Bax homodimers, which'neutralize'apoptosis induction of Bax protein.

随着Bcl-2蛋白增多，越来越多的Bax蛋白同源二聚体被分开，与Bcl-2蛋白结合形成比Bax-Bax蛋白更稳定的Bcl-2-Bax蛋白杂二聚体，从而&中和&Bax蛋白的诱导细胞调亡作用。

The Pncps-protein conjugants prepared were helpful to promote the transfer of the classes of antibody to Pncps from IgM to IgG in serum. Under our experimental conditions, when the usage of Pncps was identical in the conjugants, the more protein conjugated, the more IgG titers to Pncps and to protein were observed.

本实验制备的荚膜多糖－蛋白的结合物中，相同多糖用量情况下，蛋白用量的增加能够促进血清中抗多糖抗体类型由IgM向IgG转变，分别提高结合物抗多糖、抗蛋白IgG抗体滴度。

The RhoA oncogene , have been revealed in recent years, are closely related to various malignant tumours. RhoA, belonging to Rho family, is one member of the ras superfamily, whose encoding production, namely RhoA protein, pertaining to micromolecular mass GTP conjugated protein family, in GTP conjunctive active form and GDP conjunctive unreactive form. It exist in the latter form in normal cells and the switch of the two forms enables them to educe functions, paralleling"molecular switch", the diminutive G protein in activated state being able to activate the wink dromo-iter to work functions, which is in the resemblance of process during which the Ras genic mutation induces its continous activations.

RhoA是近年来新发现的与多种恶性肿瘤密切相关的癌基因，RhoA属于Rho亚家族，是ras基因超家族的一员，其编码产物RhoA蛋白属于小分子量GTP结合蛋白族，分为GTP结合的活性型和GDP结合的非活性型两类，在正常细胞内以GDP结合的非活性型存在，两种活性形式之间的转换使他们能够发挥一种类似&分子开关&的作用，活性态的小G蛋白能激活下游的信号传导通路，行使其功能。

Methods Physiological and biochemical parameters of the blood were determined to detect liver function. membrane protein and nuclear protein were isolated from the liver tissue of BDL rats. The protein expressions of MRP2 and FXR were determined by Western blot.

用胆总管结扎术方法制备大鼠梗阻性黄疸模型，检测肝功能；提取大鼠肝细胞膜蛋白与核蛋白，测定提取液蛋白浓度；采用Western blot技术检测MRP2和FXR蛋白表达。

Plunder melds and run with this jewel!

掠夺melds和运行与此宝石！

My dream is to be a crazy growing tree and extend at the edge between the city and the forest.

此刻，也许正是在通往天国的路上，我体验着这白色的晕旋。