查询词典 protein-free

Result Nutrient components could induce corresponding gastrointestinal digestive enzymes of crayfish at dietary protein or cellulose level of 0, 33%, 50%, comparing to starch 0, 33%; Animal protein and cellulose at high level were beneficial for the SOD, while starch was opposite. Liver glycogen of crayfish fed with high-protein diets were even higher than these with highcarbohydrate diets, meanwhile, the muscle glycogen apparent consistent with the content of carbohydrate. Overall, crayfish was well adaptable to change in the dietary composition.

结果］饲料营养组分对鳌虾肠胃消化酶有良好的诱导作用，即：动物蛋白和纤维素均在0、33%、50%时可引起相应消化酶的适应性上升，而淀粉仅在0、33%时可引起淀粉酶上升；较高的动物蛋白和纤维素有益于肌肉中SOD的提高，而高淀粉则会显著降低鳌虾SOD值；肝糖原在高蛋白情况下能不受饲料中淀粉含量下降的影响，肌糖原则与淀粉含量保持一致；各生化指标经一定天数的波动后趋于稳定。

I developed bee protein feed high in protein, fats, minerals, fat soluble vitamins, the proportion of suitable water-soluble vitamins, feed ratio of computational science, in line with bee nutritional requirements; feed is also appropriate to add grape oxidase, amylase, invertase, protein hydrolytic enzymes, fat bees enzymes necessary enzymes, raising bees on nutrient absorption rate, while reducing excretion, so that more fully reflects the use of nutrition.

我开发蜂蛋白饲料高蛋白质，脂肪，矿物质，脂肪和水的比例适当的水溶性维生素，饲料比例的计算科学，与蜜蜂营养要求，饲料也应加重葡萄氧化酶，淀粉酶脂溶性维生素，蔗糖酶，蛋白水解酶，脂肪酶的必要蜜蜂酶，提高对养分的吸收率蜜蜂，同时减少排泄，因此更充分地反映了营养的使用。

The 1-propanol/biuretium flour protein fractionation procedure based on the differential solubility of wheat flour protein in different aqueous solutions of 1-propanol yields three protein fractions, one monomeric in nature and two polymeric fractions corresponding to soluble glutenin, insoluble glutenin. Quantitative of the fraction was made with improved biuret reaction.

丙醇—双缩脲改良测定法用不同浓度的丙醇溶液将小麦面粉蛋白质分离为功能截然不同的单体蛋白、可溶性谷蛋白和不可溶性谷蛋白三部分，然后通过改良双缩脲测定法对不同组分进行定量分析。

With foaming degree of egg-white protein as response value, the optimum modification conditions for egg-white protein by xanthan were determined as follows: concentration of egg-white protein was 5.6%, addition amount of xanthan was 0.29%, the temperature was 45℃, the heating time was 22 min and the whipping time was 3.3 min.

以改性蛋清蛋白的发泡度作为响应值，对改性条件进行优化，最终确定了黄原胶对蛋清蛋白的最佳改性条件为：蛋清蛋白浓度为5.6%，黄原胶添加量为0.29%，加热温度为45℃，加热时间为22 min，搅打时间为3.3 min。

Apositive correlation between the expression of TSG101 protein and MDM2 proteinpresents in the lung carcinoma when P53 gene doesnt mutate. But when P53 genemutates, the positive correlation absents. 3. Co-detecting the expression of TSG101protein, MDM2 protein and P53 protein in lung carcinoma helps to judging the degreeof malignancy.

在P53没有突变的情况下，TSG101与MDM2的表达呈正相关关系；当P53突变时，这种关系消失。3、联合检测TSG101、MDM2和P53有助于判断肺癌的恶性程度。

The objectives of this study are:(1) To amplify Tb wbbL gene encoding rhamnosyl transferase bypolymerase chain reaction from the genomic DNA ofM.tuberculosis H37RV strain;(2) To clone PCR product of Tb wbbLgene into a cloning vector pMD18-T for sequencing;(3) To subcloneTb wbbL gene to an expression vector pET16b to construct pET16b-Tb wbbL; and to overexpress Tb WbbL protein in E.coli BL21(DE3)under different induction conditions;(4) To establish theco-expression system for expressing chaperons of pKJE7 plasmidand soluble Tb WbbL protein in E.coli BL21(DE3) under differentinduction conditions;(5) To test expressed WbbL protein by SDS-PAGEand Western blot methods.

本论文的目的是：(1)利用 PCR 方法从结核分枝杆菌 H37Rv 菌株的基因组DNA 中扩增出 Tb wbbL 基因；(2)将 Tb wbbL 基因克隆到pMD18-T 克隆载体中，经 DNA 序列测定证实为正确的基因；(3)将 Tb wbbL 基因亚克隆到 pET16b 表达载体中并通过改变不同的诱导条件在大肠杆菌 BL21(DE3)中表达 Tb WbbL 蛋白质；(4)建立在 BL21(DE3)大肠杆菌中共表达 Tb WbbL 蛋白质与分子伴侣的体系，优化表达条件以高效表达出可溶性 Tb WbbL蛋白质；(5)用 Western blot 方法鉴定所表达的 Tb WbbL 蛋白质为 Tb wbbL 基因产物。

In this experiment, a new method was used to purify the toxin protein produced by Verticillium dahliae. The supernatant of fungus culture was frozen and dried with Lyophilizer first, and then dialysed by Dialysis Membranes (MWCO 1000) after dissolved in distilled water. This method can eliminate the salt and sucrose in culture medium and reserve the protein component almost completely. VD-toxin were analysed using sodium dodecyl sulfate polyacrylamide gel analysis. The results indicated that the protein components were very complex, and included glycoprotein within 35.8kDa-83.2kDa. Furthermore, treatments on glycoproteins with high temperature, conA and proteinase alleviated, not abolished, their activities.NO and H_2O_2 production were assayed in two cultivars of cotton cells which were treated with VD-toxin.

本实验首先确定了用冷冻浓缩后透析的方法初步提纯棉花黄萎病菌毒素，该方法能有效去除粗提毒素中的蔗糖和盐离子并能最大程度的保留黄萎病菌分泌的蛋白成分；对毒素蛋白成分鉴定结果表明蛋白条带多，每种蛋白的含量少，小分子量蛋白含量多，糖蛋白染色结果显示，毒素蛋白中分子量在35.8kDa-83.2kDa之间的蛋白大多数为糖蛋白，分子量小于35.8kDa的蛋白多为非糖蛋白；性质鉴定结果显示，毒素中有活性的蛋白成分具有部分可耐高温高压，且毒素蛋白中的蛋白成分和糖基成分都具有致萎活性。

In one embodiment of the invention, anion exchange chromatography of the vegetarian Protein A media produces a vegetarian Protein A eluant that contains an at least 95% pure Protein A.

在本发明的一个实施方案中，植物蛋白A培养基的阴离子交换色谱产生含有至少95％纯蛋白A的植物蛋白A洗脱液。

The seed embryoes were penetrated by 〓 ion beams of 75 MeV/u and the seed endosperms were implanted by the proper degraded 〓 ions, and soluble protein of the seedling in M1 generation was divided into 5 sections to analyze their changes at different molecular weight. 1 In comparison with control, the relative content of the second section (molecular weight is heavier in all sections) of all irradiated materials got down, while the relative content of the fifth section (molecular weight is the lightest) of those went up. 2 The penetrating seeds made the relative content of the first section (molecular weight is the heaviest) and the third section (mid-molecular weight) drop, while the fourth section went up. Moreover, the changes of the third section and the fourth section of the penetrated materials were clearly different from those of the implanted materials. 3 The difference between implanted embryo and implanted endosperm existed in deeper dropping of the first section and higher rising of the fifth section on relative content of soluble protein of the latter. 4 On irradiated materials at lower doses, there were exceptional changes of soluble protein, it may be thought that there was excited effect of low dose.

1.75MeV／u 〓O〓离子经过适当降能后注入小麦种子的不同部位和贯穿整个种子，对其M1代幼苗的可溶性蛋白质组分以及电泳中不同区段蛋白质组分相对含量加以分析，结果表明：1）同对照相比，随着辐照剂量的增加，所有辐照材料第二区段蛋白质组分的相对含量下降；而第五区段蛋白质组分的相对含量升高。2）种子的贯穿处理，同时也引起第一区段和第三区段蛋白质组分相对含量的下降以及第四区段的升高；其中第三、四区段的变化明显区别于注入效应。3）注入胚和胚乳的区别在于后者第一区段蛋白质组分相对含量的较大下降和第五区段的较大提高。4）小剂量辐照的材料，可溶性蛋白质组分的变化异常，可能与低剂量辐射兴奋效应有关。

Protein phosphorylation catalysed by protein kinase is the predominant form of signaling in both procaryotes and eucaryotes, Protein Ser/Thr and Tyr kinase play essential roles in signal transduction.

蛋白质激酶催化的蛋白质磷酸化是原核和真核生物中一种主要的信号传导方式，蛋白质Ser／Thr和Tyr激酶在信号传导过程中起着必需的作用。1993年，张承才在Anabaena sp。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。