查询词典 protein-free

In Part 1 of our research, BGSCs were sorted through immunomagnetic beads marking by CD133 and cultured in vitro, and character as a stem cell was identified by stem cell markers (CD133 and Nestin) and differentiated cell markers [ microtubule-associated protein 2(MAP2), glial acidic fibrillary protein and myelin basic protein] , ultrastructure observing with electron microscopeand engrafting to severe combined immunodeficiency mice for tumorigenesis test. The results were as following: Only a small subset of CD133+ glioma cells in glioma cell lines and fresh specimens from various pathologic grade could express stem cell markers CD133 and Nestin, view ultrastructure of a stem cell and be capacity of serial passage in culture. These CD133+ cells possese a marked capacity for multipotent differentiation and could differentiate into tumor cells expressing MAP2,β-TubulinⅢ, GFAP and MBP; When engrafted into SCID mice, they can generate and form tumors that phenotypically resembl the tumor from the patient.

在本课题中，在第一部分实验中采用以CD133为标志的免疫磁珠法从人脑胶质瘤组织和细胞株中分离脑胶质瘤干细胞并进行体外培养，通过免疫荧光技术检测干细胞标志物CD133、Nestin，诱导分化后检查分化细胞标志物MAP2、GFAP、MBP以及电镜超微结构观察和移植SCID鼠致瘤实验，对其干细胞特性加以鉴定，得到如下结果：不同病理分级的新鲜胶质瘤标本和胶质瘤细胞株中存在一小部分CD133+的胶质瘤细胞，能表达干细胞的标志物CD133和Nestin，符合干细胞的超微结构特点，体外培养能连续传代；具有多向分化潜能：诱导分化后能产生MAP2、β-TubulinⅢ、GFAP、MBP染色阳性的细胞；移植SCID鼠后能形成与亲本肿瘤表型一致的移植瘤。

The shorter variant mHemk2 is 1696 bp in length encoding a putative mouse protein of 14.7 kDa, consisting of 138 amino acid residues.In protein data bank annotations, Hemk has previously been classed among methyltransferases methylating N6-adenine or N4-cytidine in DNA because of the presence of S-adenosyl-L-methionine binding motif and of an NPPY motif in the protein.

在蛋白数据库的注释中，由于在Hemk蛋白中存在S-腺苷-L-甲硫氨酸结合基序和一个NPPY基序，因此，在蛋白库的说明中已经将Hemk蛋白归类于能甲基化DNA中N6-腺嘌呤或N4-胞嘧啶的甲基转移酶。

The results showed that spring wheat with plastic film mulches was able to improve the content of chlorophyll and protein of leaves in the earlier growing period and the average increase per day of the content of chlorophyll and protein of the mulched wheat increased by 31.49% and 7.94% than that of unmulched wheat respectively; but it could accelerate the decomposition of chlorophyll and protein in the later period.

结果表明，采用地膜覆盖栽培方法，在春小麦生长前期能明显提高叶片叶绿素和蛋白质的含量（日均增加量分别比露地春小麦高31.49%和7.94%），但后期又会加速其降解速率；MDA含量和细胞质膜相对透性均表现为覆膜处理高于露地处理（日均增加量分别比露地春小麦高71.96%~11.78%和2.42%~9.57%）。

The expression amount of the target protein is improved by three to ten times according to different target protein, different multiplicities of infection, different protein harvesting times and different consumptions of culture medium.

依不同目的蛋白，不同感染复数，不同蛋白收获时间及不同培养基使用量，目的蛋白表达量增加3～10倍。

BACKGROUND: Protein kinase A signaling has long been known to play an important role in cardiac function. Dysregulation of the protein kinase A system, caused by mutation of the protein kinase A regulatory subunit gene PRKAR1A, causes the inherited tumor syndrome Carney complex, which includes cardiac myxomas as one of its cardinal features.

背景：长期以来，蛋白激酶A信号途径被认为在心功能中起着重要作用，蛋白激酶A调节亚单位基因PRKAR1A的突变导致蛋白激酶A系统失调，引起遗传性肿瘤综合征－Carney合成，其中心脏粘液瘤作为其主要特征。

Background— Protein kinase A signaling has long been known to play an important role in cardiac function. Dysregulation of the protein kinase A system, caused by mutation of the protein kinase A regulatory subunit gene PRKAR1A, causes the inherited tumor syndrome Carney complex, which includes cardiac myxomas as one of its cardinal features. Mouse models of this genetic defect have been unsatisfactory because homozygote null animals die early in development and heterozygotes do not exhibit a cardiac phenotype.

背景——蛋白激酶A信号传导在心功能中发挥了重要作用早已为人们所知蛋白激酶A调节亚基因PRKAR1A的变化引起蛋白激酶A系统失调，导致了遗传性卡尼复合肿瘤综合症，其中包括的心脏粘液瘤作为它的一个主要特征此种遗传缺陷的小鼠模式仍然不能令人满意，因其纯合体空白动物在发展中早期死亡，而杂合体不能显示心脏表型

Morphologically similar lesions from 26 patients (mean age, 62 y; range, 46 to 88 y; male/female ratio, 10/16) were retrieved from surgical pathology and consult files. Clinical and endoscopic data were obtained, and immunohistochemistry for S-100 protein, glial fibrillary acidic protein, neurofilament protein, epithelial membrane antigen, claudin-1, CD34, smooth muscle actin, and KIT was performed. The findings were compared with those in mucosal biopsies of 5 submucosal neurofibromas from NF1 patients.

我们从外科病理和档案库里调取了26例病变形态相似的病例（患者平均年龄62岁，范围：46至88岁，男女比例：10/16），得到了他们的临床及内窥镜资料，并给它们都做了S-100蛋白、胶质纤维酸性蛋白、神经丝蛋白、上皮膜抗原、闭合蛋白-1（claudin-1）、CD34、平滑肌肌动蛋白和KIT的免疫组化，所得的结果都与5例NF1患者粘膜下神经纤维瘤的粘膜活检的结果相比较。

The results indicated that the glass slide modified with agrose has the strongest ability to combine Protein. 2. Protein microarray in TORCH infection diagnosis Protein microarrays for detection of TORCH antibodies in human sera were fabricated by printing monoclonal antibodies on agarose film coated glass slide. The sporing concentration, specificity and cross-reactivity were investigated.

蛋白质芯片在TORCH检测中的应用研究 TORCH是指发生在孕期的各种微生物感染所致的一类临床综合症，包括弓形体(Toxoplasmagondii，Tox)、风疹病毒(Rubellavirus，RV)、巨细胞病毒(Cytomegalovirus，CMV)、单纯疱疹病毒(Herpessimples Virus，HSV)及其他感染（Others，包括微小病毒属、人免疫缺陷病毒、肝炎病毒、梅毒等）。

Heart-type fatty acid-binding protein,a 14 000～15 000 protein,is a low molecular weight protein which is abundantly present in heart tissue.HFABP plays an important role in the intracellular transportation of fatty acid,the metabolism of lipids,regulating cell proliferation and differentiation,etc. It can be used as a good biological marker for detection of acute myocardial infarction.

心肌型脂肪酸结合蛋白是一种低相对分子质量的蛋白质，其相对分子质量为14 000～15 000，在心肌中含量丰富。H-FABP在脂肪酸的运输、脂类的代谢、调节细胞增殖与分化等方面具有重要的作用，是检测急性心肌梗塞的有效生物学标志物。

On this paper, DE of PLM to goat, protein digestibility and digestable protein content were determined, and rumen degradation characteristics of dry matter and protein in alfalfa meal and PLM, and the feeding result of PLM to lamb were studied.

本论文测定了柠条叶粉对羊的消化能、蛋白质消化率和可消化蛋白质含量；比较研究了柠条叶粉和苜蓿草粉的干物质和蛋白质的瘤胃降解特性；探索性的研究了柠条叶粉对羊的饲喂效果。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。