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However, dexamethasooe did not seem to affect the sustained current and the sustained component of the biphasic current induced by ATP. The in hibitory effect of dexamethasonc on ATP induced currents was blocked by glucocorticoid receptor antagonist RU38486 (10μmol/L) and protein kinase A inhibitor H-89 (10μmol/L), but not by G protein inhibitor GDP-β-S (0.2mmol/L) and protein kinase C inhibitor chelerythrine chloride (10μmol/L).

地塞米松对ATP激活电流的快速抑制作用可被糖皮质激素受体拮抗剂RU38486(10μmol/L)、蛋白激酶A抑制剂H-89(10μmol/L)阻断,而G蛋白激活抑制剂GDP-β-S(0.2mmol/L)、蛋白激酶C抑制剂Chelerythrine chloride(10μmol/L)对地塞米松的快速抑制作用无明显影响。

Objective To study the expression of monocyte chemotactic protein-1(MCP-1)mRNA and protein induced by advanced oxidation protein productsin smooth muscle cells.

目的 研究晚期氧化蛋白产物对血管平滑肌细胞单核细胞趋化蛋白-1表达的影响。

Results The levels of HSP 70 mRNA and HSP 70 protein expression were significantly different among the 5 groups. Ketamine induced marked HSP 70 mRNA and HSP 70 protein expression in the posterior cingulated cortex. Propofol itself did not induce HSP 70 gene expression in this brain region. Propofol significantly inhibited ketamine-induced HSP 70 mRNA and HSP 70 protein expression in the posterior cingulate cortex in a dose-dependent manner.

结果氯胺酮可明显诱导HSP70 mRNA与HSP70蛋白在大鼠后扣带回皮质区的表达;异丙酚自身不能诱导HSP70基因的表达;预先给予异丙酚可显著抑制氯胺酮诱导的HSP70 mRNA和HSP70蛋白在这一区域的表达,且抑制效应呈剂量依赖性。

This result suggested that Al protein was the endemic in normal development stamens and maybe a produce of specific gene in anther tapetum, and B1 protein affected the development of stamens in female flower and maybe a important protein leading contabescence.

通过分析初步推测A1点是雄蕊正常发育特有的,可能是花药绒毡层特异基因表达的产物,B1 点可能是影响雌花中雄蕊正常发育,导致雄蕊败育的一种重要蛋白。

The soybean-protein fiber was developed by Li-Guanqi who is the member of the HuaKang group that is united with chemistry bioengineering in Pu Yang, HeNan province. The producer stuff of the soybean-protein fiber primarily contains beans dregs, hydroxyl and cyanic acid high polymer. Its production principle: First, soaking beans dregs into water and purifying out soybean-protein from the solution.

大豆蛋白改性纤维是由河南省濮阳华康化学生物工程联合集团公司李官奇等研制并生产的一种新型纺织纤维,它的主要生产原料是豆粕、羟基和氰基高聚物,其生产原理是将豆粕水浸分离提纯出蛋白质,将蛋白质改变空间结构并在适当的条件下与羟基和氰基高聚物共聚接枝,通湿法纺丝生成。

The antibacterial activities of most of fractions were similar to the pomegranate peel extract.(7) Respiratory inhibition experiment showed that the respiration of thallus was inhibited mainly through the hexose monophosphate pathway, which leaded to be short of NADPH, and induced metabolic function dysfunction. Growth curve assay showed that the pomegranate peel extract inhibited cytodieresis in logarithmic growth phase. After exposure to the pomegranate peel extract, bacterial protein was analyzed by SDS-PAGE. The results showed that protein synthesis was inhibited, especially for large molecular weight protein.

7呼吸抑制试验结果表明,石榴果皮提取物主要是通过抑制HMP途径,导致还原力NADPH缺乏,引起代谢功能障碍,达到抑菌的目的;加药组和正常组金黄色葡萄球菌生长曲线的对比结果表明,石榴果皮提取物能够抑制金黄色葡萄球菌对数生长期的菌体分裂;SDS-聚丙烯酰胺凝胶电泳分析表明,石榴果皮提取物能够抑制菌细胞蛋白合成,尤其对菌体内大分子量蛋白作用最明显。

To investigate the expression, location and function, long distance PCR was used to expand the targets containing open reading frame. The expressive vectors of prokaryotic and eukaryotic cells were constructed after product purification and connecting with vectors. The prokayotic vectors were induced to express the protein by IPTG and the protein was seen by denaturalization PAG electrophoresis and dyeing. The eukayotic expressive vectors were transfected into culture cells and the protein was found in the nulceolus under the observation of the confocal microscope. The transfected cells were chosen by the geneticin (G418). The cell cyele was examined by flow cytometer and the cell numbers were reduced obviously in the stage of G〓-G〓 and G〓-M, but increased distinguishably in the S stage.

为研究磷酸化应激诱导蛋白的表达、定位和功能,采用了长距离聚合酶链反应扩增含有开放阅读框架的靶片段,经产物纯化、与载体连接,分别构建了STIP1基因的原核表达载体和真核表达载体;IPTG诱导原核表达载体,变性聚丙酰胺凝胶电泳与染色后,可见相应大小的蛋白质表达;STIP1基因的真核表达载体转染细胞系后,共聚焦显微镜下观察STIP1蛋白主要分布于细胞核内;用药物筛选STIP1基因转染的细胞后,流式细胞仪检测细胞周期,可见G〓-G〓期和G〓-M期的细胞明显降低,S期细胞明显增多。

One protein band, which was named as abalone triploid special expression protein, was found to be distinguishingly existed in one to six years old triploid abalone by PAGE analysis. While in Pacific oyster and Zhikong scallop, there is no special expressed protein could be observed among diploid, triploid or tetraploid individuals.

用常规聚丙稀酰胺凝胶电泳技术研究发现皱纹盘鲍的二倍体和三倍体个体中存在稳定的差异表达蛋白质,命名为a-TSP;在长牡蛎的2n、3n、4n之间,及栉孔扇贝的2n和3n之间未获得类似的差异表达蛋白。

In the same tissue, the difference of the expressions of the two protein was not statistically significant. Conclution: The loss and reduction of neurexin protein, neuroligin protein in myenteric nerve plexus is an important cause of enterokinesia in congenital megacolon.

neurexin、neuroligin蛋白在人体肠神经系统肌间神经丛中表达的减少及缺失是导致先天性巨结肠肠蠕动功能丧失的重要原因。

The model of Protein Secondary Structure based on the multi-modal neural network This paper proposed a multi-modal neural network predictable model that synthesizes by 7 BP neural network group in protein secondary structure prediction, this model use "profile" encoding method and "the distance" between the amino acid for the excitory input information, has carried on the prediction to the protein secondary structure prediction.

3基于多神经网络的蛋白质二级结构预测模型论文提出了一个由 7 个 BP 神经网络组合成的多神经网络的蛋白质二级结构预测模型,该模型采用&profile&编码方式和氨基酸间的&距离&为激励输入信息,对蛋白质二级结构进行了预测。

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Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气,收缩臀部肌肉;拱起身体,尽量抬起头来,右腿伸直朝向天花板(膝微屈,以避免肌肉紧张)。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化,但却比去年同期高8%。

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