查询词典 protein-free

With the advent of post-genomic era, identification of protein-protein interaction has become another hot spot of protein research and promoted the invention, development and complement of relative techniques.

随着后基因组时代的到来，阐明蛋白质间相互作用关系成为蛋白质研究的又一热点，促进了相关技术的不断产生、发展和完善。

The synthesized cDNA is a continuous band less than 6. 0kb shown on x-film through α〓PdCTP which was inserted in the first strain of cDNA. The titer of the Ha-cDNA library was about 1. 5×10〓cfu/ml which could represent all protein-encoding sequences from Hz-AM1 cells; Namely, the cDNA library has representativity. After the successful construction of the Ha-cDNA library, the open-reading frame of vp39 gene of Heliothis armigera was then fused in frame with the Ga14-DNA-binding domain in the pGBT9 plasmid and the fusion protein Ga14-VP39 was expressed in yeast to be used as bait protein.

在成功构建棉铃虫细胞Hz-AM1cDNA文库的基础上，本文接着将棉铃虫核型多角体病毒衣壳蛋白VP39（HaNPV-VP39）基因克隆到酵母双杂交系统(yeasttwo-hybrid system)中的质粒pGBT9上。pGBT9上含有酵母菌转录激活因子GAL4 DNA结合域的编码区（GAL4-DBD），使克隆到质粒pGBT9上的HaNPV-vp39基因与GAL4-DBD基因融合，在酵母菌中表达DBD-vp39融合蛋白，该融合蛋白即为酵母双杂交系统中的诱饵蛋白。

Its current projects include: quantitative prediction of protein-protein interactions and membership in protein complexes; improved identification of peptide sequence; development of a Bayesian statistical framework for assessing and representing uncertainty in gene function annotation etc.

其最近的研究项目包括：蛋白质复合体中蛋白质-蛋白质相互作用及其成员的定量预测；经改良的肽序列鉴定方法；贝叶斯统计方法在基因功能评价和不确定性分析等方面应用的发展等。

Containing many T cell epi-positions and neutralizing antibody epi-positions, the core protein region and envelope protein 2 of Hepatitis C virus structural protein may induce protective immunity responsion in body.

丙型肝炎病毒(hepatitis C virus，HCV)结构蛋白的核心蛋白和包膜蛋白2(E2)含有多个T细胞表位和中和抗体表位，可诱导机体产生保护性免疫应答。

This is because the alfalfa products can be a variety of amino acid balance of ruminant livestock, and provide high-quality vitamins and fiber, so that rumen protein to total protein content of 58% to 60%, thus significantly increasing the efficiency of alfalfa protein utilization results.

这是因为苜蓿草产品可平衡反刍家畜的各种氨基酸，并提供维生素和高品质纤维素，使瘤胃蛋白含量达到总蛋白质的58％～60％，从而大大提高苜蓿蛋白质利用效率的结果。

Nodes of Ranvier; saltatory conduction; Schwann cells; oligodendrocytes; myelin basic protein; demyelination; remyelination; myelinopathy; myelinogenesis; myelin-associated protein; myelin sheaths; demyelinating neuropathy; myelin proteolytic protein; chronic inflammatory demyelinating polyradiculoneuropathy; multiple sclerosis; MS

郎飞氏结；跳跃传导；施旺细胞；少突神经胶质细胞；髓鞘碱性蛋白脱髓鞘；髓鞘再生；髓鞘质病；髓鞘形成；髓磷脂-关联的蛋白质；髓鞘；脱髓鞘神经病；髓磷脂溶解蛋白的蛋白质；慢性炎症性脱髓鞘多神经根神经病；多发性脑硬化；MS

Wong figured the mutated gene might make an ideal target for a vaccine, as it creates an abnormal protein not seen in healthy cells. He hooked up a fragment of the mutant protein to a larger protein from a giant sea snail that looks foreign and can rev up the immune system.

使她第11次决定戒烟的，是一次从美国北部演讲回家时，中途因遇到大雪，滞留时间较长，百无聊赖时，就抽了两支香烟，回家后竟生起病来，喉咙沙哑，发不出声，这下子可把她吓坏了。

And target protein can be secretively expressed in Pichia pastoris, this is helpful for separation and purification of target protein.Therefore this research intends to compare expression of Core protein in E. coli with in Pichia pastoris.

与大肠杆菌相比，毕赤酵母是单细胞真核生物，既具有生长快的特点，又能有效克服大肠杆菌系统缺乏蛋白翻译后加工、修饰的不足，表达外源蛋白可分泌到胞外，便于产品的分离纯化。

As a result, Core gene was expressed in E.coli with yields of 20% of total proteins and it was a 31KD fusion protein on SDS-PAGE and Western-Blot, it shows that Core protein was highly expressed in E.coli;One fragment of 369bp could be seen on the gel of the Core gene PCR product. A band of 29KD could be seen on the Western-blot after induction of yeast containing pPICZaA-Core for 72 hours. This indicate that Core protein was secretively expressed in the Pichia pastoris with weak yields.

结果显示pBVIL1-Core的表达产物经SDS-PAGE分析出现一条约31KD的带，与预期融合蛋白的分子量相符，表达蛋白存在于包涵体中且表达量占菌体总蛋白的20%，Western-blot显示诱导后菌体在相应位置出现特异性杂交带，表明

The expressed protein accounted for more than 30 % of the total protein mass. Western blot confirmed that the fusion protein reacted to positive serum against sheep pox.

在IPTG 浓度为0.1 mM、温度为37 ℃、诱导6 h时表达量最大，约占菌体蛋白的30% Western blot 试验表明，融合蛋白可被羊痘阳性血清识别，这为研究其在新型疫苗和诊断的研究奠定了基础。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。