查询词典 protein-free

A pair of primers was designed according to the coat protein gene of Arabis mosaic viru and used to amplify a 990 bp partial gene of the cp by reverse transcription-polymerase chain reaction, the 990 bp partial gene was cloned into the pEGX-9p-1, a prokaryotic expression vector fused with GST. The recombinant vector was transformed into E. coli BL21. The fusion protein was successfully expressed in E. coli BL21 induced with the IPTG at 37℃.

依据报道的ArMV外壳蛋白基因(coat Protein， Cp)序列设计引物，通过RT-PCR 扩增得到长约990bp的ArMV cp基因亲水基团部分片段，将目的片断克隆到原核表达载体pEGX-6p-1中，构建ArMV cp基因与GST蛋白融合表达载体pEGX-cp，重组载体化大肠杆菌BL21，经IPITG诱导后，融合蛋白GST-cp得到了特异表达。

This research is based on our previous successful cloning of a completely novel calcium metabolism related protein—calmodulin-like protein from pearl oyster and calmodulin. The main purpose of this research is to find out the function of CaLP on the cell level and compare functions of these two proteins.

为进一步揭示贝类生物矿化机理，本研究以目前我国用于生产海水珍珠的主要贝类合浦珠母贝为研究材料，以本实验室已克隆出的新蛋白钙调素类似蛋白(calmodulin-like protein，CaLP)为研究对象，对CaLP在细胞水平上的功能进行了进一步的研究，并将其与本实验室已克隆出来的另一钙代谢相关蛋白钙调素(calmodulin，CaM)进行了比较。

Pombe Protein kinase cascade regulates the response of extracellular stimulation in cytoplast and cell nuclei. The MAPK signal cascade is an ancestral and conservative protein kinase in eukaryote.

MAPK(mitogen-activated protein kinase，丝裂原激活的蛋白激酶)级联是在单细胞和多细胞真核生物中发现的古老而保守的蛋白激酶级联。

Lavoie JN, Hickey E, Weber LA, et al. Modulation of actin microfilament dynamics and fluid phase pinocytosis by phosphorylation of heat shock protein 27.

热休克蛋白（heat shock protein， HSP）又称应激蛋白（stress protein， SP），是机体细胞在应激原诱导下，高效表达的一组蛋白质。

The green fluorescent protein is a bioluminescent protein derived from the Jellyfish Aequorea.

绿色荧光蛋白（green fluorescent protein， GFP是一种来源于水母的生物发光蛋白。

There are 11 gene bindings disappear in the normal group, but appear in the model group, and then disappear in the treat group, that indicates the 11 gene bindings are correlated with the epilepsy and Caohuozhimutang. After searching in the Gene Bank, 7 bindings in the 11 gene bindings are homogenic with the 8q31 ribosomal protein gene, 15p12 paraneoplastic neuronal antigen gene, 4q22 diacylglycerol kinase iota gene, xq37 FMR2 protein and 11p11 roudabout homolog 1, and 4 bindings of which are novel gene.

经NCBI美国国家基因库检索，这11条基因中的7条已知基因分别与位于鼠染色体8q31位置的核糖体蛋白、15p12位置的周围肿瘤神经元的抗原(paraneoplastic neuronal antigen)、4p22位置的甘油二酯酶、xq37位置的FMR2蛋白（FMR2 protein）的基因以及以及位于果蝇染色体11p11位置的交叉同族体（roundabout homolog 1）有极高的同源性；有4条基因片段同源性较低，为功能未知的新基因，已经在GeneBank进行了注册，注册号分别CK325391、CK325392、CK325393、CK325394。

Abstract］ Objective: Study and explore the express of gene and protein of protein kinase B about cerebral ischemia effects of phenolic alkaloids from Menispermum dauricumon cerebral ischemia in rats.

目的：探讨蝙蝠葛酚性碱（phenolic alkaloids from Menispermum dauricum，PAMD）预处理对脑缺血后大脑皮质细胞凋亡蛋白激酶B(protein kinase B，PKB)表达的影响。

OBJECTIVE: To construct a novel adenoviral eukaryotic expression vector that can co-express mutant hypoxia-inducible factor-1 alpha (HIF-1α) target protein and humanized Renilla reniformis green fluorescent protein reporter molecule under normoxic conditions.

目的：构建能够同时表达突变型低氧诱导因子1α(hypoxia inducible factor 1 alpha，HIF-1α)目的蛋白和人源化绿色荧光蛋白(human renilla reniformis green fluorescent protein，hrGFP)报告分子的新型腺病毒真核细胞表达载体。

Find information about the protein's secondary, tertiary, and quaternary structure using database sites such as the Protein Data Bank or SCOP.

用数据库站点如Protein Data Bank和SCOP，找出此蛋白质的二、三、四级结构。

OLH or 8-bromo-cAMP were added to luteinized granulosa cells. Results indicated that, protein expression of HIF-1a, HIF-1b and VEGF had a better performance in hypoxia than in normoxic condition, but phospho-PKA, StAR and P450scc protein expression had weaker expression in the former condition than in the later one. As a result of decreasing secretion of progesterone the decreasing of phospho-PKA was suspected to have a role in the reduction of StAR and P450scc in the condition of hypoxia.

结果发现，黄体化粒性细胞於缺氧条件下其HIF-1a、HIF-1b与VEGF蛋白质表现量皆较正常氧高；同时，StAR及P450scc蛋白质表现量则降低，但3b-HSD不受缺氧影响；此外，藉由特异性抗体检测PKA讯息传递路径发现，PKA讯息传递路经於缺氧条件下磷酸化PKA（phospho-protein kinase A）蛋白质表现量下降，可能经此路径的调控造成StAR及P450scc蛋白质表现量下降，导致黄体化粒性细胞孕酮分泌量减少。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐