查询词典 protein-free

The preliminary structure analyzing indicated that this protein was a hydrophilic protein; comparised of Batrachuperus pachunii β-microseminoprotein precursor-like protein sequence to other speices, 10 cystenies and other 15 aminooacids were conserved and located at the same positions as those in amniote β-microseminoprotein.

初级结构分析表明，该蛋白属于亲水性蛋白；多重序列比较显示，其氨基酸序列中的10个半胱氨酸位点及15个其他氨基酸位点与高等脊椎动物β-microseminoprotein中同种氨基酸有相同的位点。

The yolk proteins were collected through the anatomization of the maiden eggs and the eggs in different embryo development stages. The yolk proteins were separated by two-dimensional electrophoresis and followed the image analysis. Over 203 protein spots were found to exist in the yolk protein of silkworm eggs. Some different patterns of yolk protein spots were found in silkworm of various varieties. While, little variation of yolk proteins was detected during different development stages of any silkworm variety.

采用直接解剖蚕卵获取卵黄蛋白，结合蛋白质双向电泳和图像分析，对家蚕处女蛾卵及不同胚胎发育阶段蚕卵的卵黄蛋白质进行了研究，发现家蚕卵黄蛋白有 203 个蛋白质斑点；不同家蚕品种之间卵黄蛋白的组成存在一些差异；同一品种不同发育时期胚胎卵黄蛋白的组成基本保持不变。

Finally, the fusion protein was expressed in E coli DH5α under induction by arabinose. And the fusion protein was 65 kD and account for 30% of the total protein of the bacteria.

SDS-PAGE电泳分析显示，融合蛋白分子量约65 kD，与预期蛋白分子量一致，并且目的蛋白占菌体蛋白的30%。

To better understand the roles of LsrA protein in nodulation, a series of analyses of nodules using scanning electron microscopy, genetic and biochemical approaches have been carried out. Our analyses suggest that the lsrA1 nodules contain bacteroids in the invasion and establishing zone only. The lsrA gene expression is active early in the invasion zone and activated later than bacA genes. The lost of LsrA protein dramatically reduced the expression of nifA and fixK, and completely blocked the expression of the nifH gene for nitrogenase. LsrA protein functions early in the bacteroid development and it is essential for the development nitrogen fixing bacteroids.

前期的工作中，苜蓿中华根瘤菌Rm1021中90个候选LysR基因已经被定向插入突变，并筛选在自生生长时期、共生生长时期的表型，以期寻找更多在自生状态或共生固氮中有功能的LysR转录因子。1 针对前期鉴定出的共生固氮必需的lsrA基因，我们应用了一系列扫描电子显微镜技术、生物化学、分子遗传学等方法，发现lsrA基因主要在根瘤侵染区开始表达，表达时序也在侵染阶段左右，但晚于bacA基因表达；LsrA蛋白缺失后根瘤固氮区中缺乏具有固氮能力的类菌体，nifA和fixK基因的转录水平降低，nifH基因的转录被完全阻断，因此LsrA蛋白为根瘤发育所必需，是新的根瘤发育信号传导途径成员。2 通过表型筛选我们鉴定了苜蓿中华根瘤菌的oxyR基因，并研究了它的调节特性。oxyR突变后，苜蓿中华根瘤菌对过氧化氢敏感性提高，适应性降低。

On the establishment of shielding immune cells avoid HIV viral antigens identified protein adsorption and the establishment of "biological trap" induce HIV models Summary : HIV is the human immunodeficiency virus caused AIDS virus, AIDS is not currently be fully human control, AIDS vaccine is being developed, but because the virus genetic diversity potential to change the topic of the complexity of this ban from the incidence of HIV spread T4 cells in the mechanisms by, proposed changes T4 lymphocyte surface protein CD4 receptors structure as to avoid HIV adsorption, and the establishment of "biological trap" booby HIV idea to try to turn on AIDS treatment to the conversion of protein structure and function, as well as synthetic direction.

提要：HIV即人类免疫缺陷病毒是引起AIDS的病毒，目前爱滋病还不能被人类完全控制，AIDS的疫苗也正在研制中，但是由于病毒多样性的遗传潜能，使这一课题变的很复杂，本文试从HIV侵染T4细胞导致发病的机理入手，提出改变T4淋巴细胞表面CD4受体蛋白构象以避免HIV吸附，并设置"生物陷阱"诱杀HIV的构想，试图把对爱滋病的治疗引向对蛋白质结构与功能转化以及合成的方向。

In recent years, much work on the characteristics of gene, protein structure and expression has been done. Up to now, people have gained considerable knowledge about the AMBN gene series of mouse, rat, pig, human and caiman, and the expression pattern of AMBN protein and mRNA. However, many problems about this protein remain unsolved yet.

近年来，不少学者在AMBN的基因特征、蛋白结构和功能、组织定位等方面展开了深入的研究，先后克隆了小鼠、大鼠、猪、人和鳄鱼等的基因序列，用免疫组化或原位杂交方法对部分种属的动物和人的AMBN或AMBN mRNA的表达进行了时空定位，并在此基础上对蛋白的功能作了初步研究。

Quite simply the most calorie-packed and best tasting weight gainer on the market today. With Muscle Juice 2544 you will grow Muscle with 55 grams of Ultimate Nutrition's special protein blend (Whey Protein Isolate, Whey Protein Concentrate, Calcium Caseinate, Egg White Albumen).

快速简单的使更多卡路里装载以及最佳风味的增肌产品在当今市场，用肌肉果汁增肌粉你将增高肌肉于55克最终营养特定蛋白质配方(乳清蛋白、隔离乳清蛋白、浓缩乳清蛋白、酪酸钙、鸡蛋蛋白)。

Results Eleven proteins, including chymotrypsinogen B1 precursor, carboxypeptidase A1, trypsinogen 2, chymotryptic peptide C, trypsin 1, carboxypeptidase B1, kinesin superfamily proteins 3B, trypsin 2, mitochondria protein gene, elastase 3A and colipase were found to be able to bind to HCV core protein. Conclusions Proteins related with metabolism of glucose and lipid may bind to HCV core protein.

因此我们应用酵母双杂交技术筛选人胰腺细胞 cDNA 文库中的 HCV 核心蛋白结合蛋白基因，为研究 HCV 慢性感染与胰岛素抵抗（ insulin resistance ， IR ）、代谢性疾病之间的相关性及其作用机制，以及进一步研究 HCV 影响糖、脂类代谢的分子生物学机制提供一定的思路和方向。

Results In the course of interaction between organism protein and crocidolite, the protein symmetry decreased and structure loosened. The silicon of crocidolite was bonded with the alkyl, amido- of protein. New absorption bands of Si-O-C, Si-R clearly appeared.

结果 机体蛋白质在与青石棉的相互作用过程中，组织结构对称性降低；青石棉的矽已与蛋白质中的烷基、胺基交联成键，存在明显的Si-O-C、Si-R新的吸收谱带。

PartⅡTransform the yeast, screen the excellent expression yeast strain, induce the yeast to express the fusion protein and depurate the target protein. The recombination plasmid was transformed into the yeast GS115 successfully by means of electrotransformation. The excellent yeast strain was got by G418 screening. We achived the yeast supernatant including target protein induced by methanol.

第二部分：酵母的转化、蛋白高表达菌株的筛选、重组蛋白的诱导表达及纯化实验利用电转化的方法，成功地将重组质粒pPIC9k-PTD-hA20导入毕赤酵母GS115，并通过G418浓度梯度筛选得到了蛋白高表达菌株，并对蛋白高表达菌株进行甲醇诱导表达，得到了含有目的蛋白的酵母上清液。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。