查询词典 protein-free

Grain quality affected by N application and plant density could directly influence malting quality. Grain protein content was negatively correlated to steeping degree and steeping rate, and positively related to saccharogenic power. The relationship of grain protein content to a -Amino-nitrogen content and kolbach index could be expressed with a quadratic curve equation, respectively. The greatest decomposition rate of protein might be achieved when grain protein content was 11-12%.

施氮量、氮肥运筹比例和密度对籽粒品质的影响直接影响着麦芽品质，籽粒蛋白质含量下降，浸麦度和浸出率上升，糖化力下降，籽粒蛋白质含量和库值、氨基氮呈二次曲线关系，籽粒蛋白质含量在11%一12%时，其蛋白质分解速率最快，麦芽总氮与籽粒总氮呈极显著的正相关关系，在麦芽的生产过程中氮的消耗率与籽粒总氮为极显著的负相关关系。

The results indicated that immobilized E2 protein remarkably raises the level of phosphorate-tyrosine in Raji cells , but the soluble E2 protein hasn't obvious impact to phosphorylation of tyrosine of Raji cell. It was presumed that immobilized E2 protein was resembled to natural viron enveloped E2 protein and may more efficiently interact with CD81 molecule.

这可能是因为固相化的E2蛋白类似天然的病毒颗粒，在构象上要更接近于天然的病毒颗粒表面E2蛋白，而且固相化后的E2蛋白在单位体积的密度要高于可溶性的E2蛋白单位体积的密度，使其能够更充分的与细胞表面的CD81分子相互接触作用。

Complete genome sequences of 20 viruses in Bunyaviridae loaded from NCBI were analyzed by two biological softwares of DNAMAN and DNASTAR. The results indicated great variability in both nucleic acid sequence and protein structure between plant viruses and animal viruses:(1) only plant viruses could encored NSm in M genome;(2) the length of nucleic acid sequence and protein sequence was different;(3) GC content of nucleic acid of animal viruses was higher than plant viruses of that;(4) protein topology analysis by online software SMART discovered that there was significant difference for the structure of glycoproteins G(subscript nG between animal-infecting and plant-infecting viruses;(5) usually, protein of plant-infecting virus had more complicated structure, more low compositional complexity and had N-Signal peptides except INSV.

本文从NCBI数据库下载具有完整基因组序列的布尼亚科病毒的5个属20种病毒的序列，用生物学软件DNAman，DNAstar进行比对分析，发现布尼亚科病毒属中的植物病毒在核酸序列及蛋白结构上与动物病毒有很大差异：(1)只有植物病毒的M基因组能编码NSm运动蛋白；(2)植物病毒和动物病毒在核酸序列和蛋白质序列长度均有差异，表明该科病毒是进化速度较快的病毒；(3)植物病毒核酸序列的GC含量低于动物病毒；(4)通过SMART网络软件进行蛋白质拓扑结构分析发现植物病毒和动物病毒在糖蛋白GG的结构上存在显著差异；(5)植物病毒糖蛋白结构较为复杂，有较多的紊乱区域，除INSV外，其他病毒都具有N端信号肽。

MS detection showed the six protein spots were inorganic pyrophosphatase, peptidyl-prolyl cis-trans isomerase A, elongation factor Tu, unnamed protein, dioxygenase and moxR protein. Conclusion Protein expression differences were discovered between multi-drug-resistant Mycobacterium tuberculosis isolate and drug-sensitive isolate.

质谱分析发现，6个蛋白质点分别代表的蛋白质为无机焦磷酸酶、多肽脯氨酰基顺反同分异构酶A、延伸因子Tu、420个氨基酸的未知功能蛋白质、双加氧酶和转录调节蛋白。

Specific protein bands were observed in cellular and extracellular protein electrophoresis for both strains 593 and 18.There were also different protein expression in certain protein bands.It is indicated that a significant difference existed between the proteome of strains 593 and 18.This difference may contribute to the different cariogenic potential of these two strains.

两菌株的菌体和菌外可溶性蛋白电泳图谱中均可见各自特异表达的蛋白条带，且部分相同位置的蛋白条带在蛋白表达量上也存在差异，表明两菌株的蛋白组存在明显差异，该差异可能是导致其致龋力差异的原因之一。

At concentration of 2% PEG8000, 30% microsomal protein containing 10% of total P450 was precipitated. At concentration of 5% PEG8000, 55% microsomal protein containing 20% of total P450 was precipitated. At concentration of 8% PEG8000, 78% microsomal protein containing 30% of total P450 was precipitated. At concentration of 11% PEG8000, 85% microsomal protein containing 70% of total P450 was precipitated.

当PEG的浓度为2％时，约30％的微粒体蛋白和10％的P450被沉淀；PEG为5％时，约55％的微粒体蛋白和20％的P450被沉淀；PEG为8％时，约78％的微粒体蛋白和30％的P450被沉淀；PEG为11％时，约85％的微粒体蛋白和70％的P450被沉淀。

The deleted mutant PAP gene was also cloned into yeast secreted expression pPIC9K vector to form pPIC9K~3, then the vector was transferred into Pachia pastoris GS115 strain. The specific expression protein was secreted into the medium after inducing with methanol and the protein amount reached about 50-60 U g per millilitre measured by UV-absorbed methods in the supernatant of the medium via high density fermentation. SDS-PAGE results showed that there was one protein band in the gel which molecular weight was about 34Ku . The protein could specific react with France PAP antiserum in Western-blotting. Activity tests revealed t

将缺失型PAP基因克隆于酵母分泌型表达载体PPICgK构成重组载体，然后导入毕赤酵母（P8chia nastoris）菌株GSlls细胞中，在甲醇的诱导下，经过酵母高密度发酵进行PAP的表达，经SDS－PAGE分析，结果表明，在培养基上清液中含有一明显的特异性蛋臼条带，大小为34Ku，经Western－blotting分析，该蛋白与法国PAP抗血清有特异性反应，体外活性检测表明该蛋白对TMV的侵染性具有高度的抑制性，说明该 PAP基因在毕赤酵母 GS中也得到了正确表达。

Woodchucks . The HBV genome has four genes: pol, env, pre-core and X that respectively encode the viral DNA-polymerase, envelope protein, pre-core protein (which is processed to viral capsid) and protein X. The function of protein X is not clear but it may be involved in the activation of host cell genes and the development of cancer.

乙型肝炎病毒基因组有四个基因：波兰，环境，前核心和X ，分别编码病毒DNA聚合酶，包膜蛋白，前核心蛋白和蛋白质十大功能蛋白X是尚不清楚，但它可能参与激活宿主细胞的基因和癌的发展。

And objective parameters (texturized degree, color, hardness, springiness, chewing, water absorption, yield, etc.) were investigated in the high moisture soy protein extrusion process, and the statistical models of system and objective parameters were built, using the step-by-step regression analysis; A comprehensive evaluation of the products, and the process optimization of high moisture extrusion were obtained,using factor analysis; Secondly, residence time distribution and soy isoflavones losses kinetics were studied in the high moisture extrusion; Finally, chemical bonding process, the micro-structure and protein secondary structure changes were investigated in the texturization of soy protein, then the mechanism assumptions of texturization soy protein by moisture extrusion were proposed.

本文对大豆蛋白高水分挤压组织化技术和机理进行了研究，研究内容包括：首先采用系统分析法，研究了大豆蛋白高水分挤压过程中，挤压机操作参数(螺杆转速、物料水分、喂料速度和机筒温度)对系统参数(系统压力、扭矩、单位机械能等)、目标参数(组织化度、色泽、硬度、弹性、咀嚼性、吸水率、产量等)的影响规律，建立了各因变参数的统计模型；采用因子分析法对产品进行了综合评价，并对高水分挤压组织化工艺进行了优化；其次，研究了大豆蛋白高水分挤压中的停留时间分布以及大豆异黄酮的损失动力学；最后，研究了大豆蛋白组织化过程中化学键、微观结构和蛋白质二级结构的变化规律，提出了大豆蛋白高水分挤压组织化的机理假设。

The protein interaction domains was delineated at the N-terminal 50-amino-acid fragment of HCV core protein and the C-terminus of p53. Confocal analysis also revealed that these two proteins colocalize in subnuclear granules and peri-nuclear region. Transfection experiments using a p53-responsive reporter plasmid in HuH-7, Hep3B, HepG2 and H1299 demostrated that full-length HCV core protein could elicit a positive or negative effect on the p53-mediated transcriptional activation depending on the concentration of the HCV core protein.

更且利用p53蛋白C端删除55-95个胺基酸之质体与含p53蛋白结合区之报导基因和表现全长HCV核心蛋白三者之质体於H1299细胞株进行共同转染时，与全长p53蛋白比较，发现HCV核心从增强全长p53蛋白之转活化能力转为抑制p53蛋白缺乏C端55-95个胺基酸之转活化能力，因实验室已有结果证实活体外及活体内缺乏C端55个及75个胺基酸之p53蛋白无法与HCV核心蛋白进行结合(Kao， unpublished data)。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐