查询词典 protein-free

We studied the effects of protein content, pH and ionic strength on viscosity of silver carp myofibril protein by orthogonal rotational regression design, and established the regression equation of silver carp myofibrillar protein solution viscosity with protein content, pH and ionic strengt

利用二次回归正交旋转试验设计，研究了蛋白质浓度、pH、离子强度对鲢鱼肌原纤维蛋白粘度的影响，建立相应的回归方程，分析比较了它们间的相互作用关系，确定了取得最优粘度时，各因素的组

The sulphur-rich protein encoding genes are transferred into crowtoe to improve the content of the sulphur-containing amino acids of forage protein through genetic engineering. Zeins, maize prolamin, the major protein reserves of maize kernels, are accumulated in protein bodies arising from the rough endoplasmic reticulum.

通过分子克隆及遗传转化等方法，将外源高含硫氨基酸蛋白基因转入百脉根，提高牧草蛋白中含硫氨基酸含量具有十分重要的意义。

According to the protein suppplementary action, the soy protein isolate and Whey Protein Concentrate was mixed in an optimal proportionment, in order to make sure the contents of essential amino acid were enough for the human demand, and that the mixed protein powder has a good quality, and can be absorbed easily.

本研究依据动植物蛋白互补原理，将大豆分离蛋白和乳清蛋白按一定比例混合，使其9种必需氨基酸的含量更接近人体所需的氨基酸模式，极易为人体吸收和利用，且具有良好的稳定性和感官品质。

Yam steroid saponins and sapogenin are resulting in MCF-7 cells apoptosis by arrested the G0/G1 phase, increased the p53 protein with promoting apoptosis and decreased the AKT protein with anti-apoptosis and the BRCA-1 protein with repairing DNA. And, SPG has the excellent anti-cancer effect on MCF-7 cells may be due to induced much of the p53 protein in the early treated stage.

进一步的研究发现，山药固醇类皂及皂元抑制MCF-7乳癌细胞的功效，主要是诱发G0/G1期停滞，造成细胞凋亡；其透过的机制是经由p53促凋亡蛋白的增加，及AKT与BRCA-1等抗凋亡蛋白被抑制，达到双重细胞凋亡之抗癌效果；其中SPG处理之MCF-7肺癌细胞具早期大量诱发p53蛋白的特性，或许是SPG优异抗癌效果之原因。

By means of essencial amino acid index and animal growth experiments, using the amino acid composition of the muscle in black sea bream juvenile as standard EAA requirement model for fish feed, The effects of nine different formulae of feed protein sources were compared. The amino acid balance and the nutritional value of the protein sources in feed for the juvenile of black sea bream were studied. Two formulae of protein sources were selected to be most effective, It suggests EAAI be an effective and simple assessment standard in studies on valuation of amino acid balance in protein sources of fish feed.

以黑鲷劫鱼肌肉的氨基酸组成作为幼鱼饲料中必需氨基酸需要量的标准模式，通过必须氨基酸指数和生物饲养试验结果相结合，对黑鲷幼鱼的9种不同蛋白源配比的饲料进行了比较研究，对黑鲷幼鱼配合饲料中氨基酸平衡和蛋白质营养价值进行了评估，筛选出2个高效饲科蛋白源配方；表明EAAI是鱼用饲料蛋白质营养价值评估和氨基酸平衡研究的一种简便有效的评价指标。

objective to study gastrodia elata blume to effect tau protein,sod,mda expression in gyrus hippocampi and cerebral cortex of experimental mice.methods to inject okadaic acid to mice ventriculus lateralis and to measure tau protein level,sod activity and lipid superoxide mda content of sod control group,oa injection group,oa and gastiodia elata injection solution group.results tau protein of experimental group(p＜0.05/p＞0.05),sod was lower than model group(p＜0.001) and was higher than control group(p＜0.05).conclusion gastrodia elata blume can increase sod activity and reduce tau protein expression and superoxide lipid forming in brain tissue of experimental dementia mice caused by oa.it can prevent and treat ad.

目的 观察天麻对痴呆模型大鼠海马、皮质神经元微管相关蛋白、超氧化物歧化酶和脂质过氧化物丙二醛表达的影响，探讨其治疗阿尔茨海默病(alzheimer disease，ad)的作用机制。方法用冈田酸(okadaic acid，oa)注射大鼠侧脑室造模，测定模型组、实验组、对照组海马和皮质tau蛋白、sod、mda的含量。结果实验组tau蛋白低于模型组(p＜0.001)，高于对照组(p＜0.05)；sod高于模型组(p＜0.001)和对照组(p＜0.05)；mda低于模型组(p＜0.001)，高于对照组(p＜0.05)。结论天麻可增强oa致实验性痴呆大鼠脑组织sod活性，降低mda蓄积和tau蛋白生成，具有防治阿尔茨海默病的作用。

RESULTS: A well-resolved and reproducible 2-DE pattern of PBMC was obtained from patients with HBV associated HCC or LC. For HCC, the mean number of protein spots was 1186±43, with an average matching rate of 91.6%, and for LC, the mean number of protein spots was 1013±41, with an average matching rate of 90.2%. Twenty-seven differential proteins were tested by MALDI-TOFMS, and 16 were identified. Of the 16 proteins, 13 were up-regulated in patients with HCC, including heat-shock protein 27, member RAS oncogene family (RAB14), actin, alpha 1 antitrypsin, RNAbinding protein regulatory subunit and so on. By contrast, the levels of proteins such as PDX6, HSPA8 and MnSOD were significantly reduced in HCC patients.

结果：得到了分辨率较高、重复性较好的HBV相关性HCC及LC患者PBMC双向凝胶电泳图谱，HBV相关性HCC及LC患者PBMC凝胶的平均蛋白质点数分别为1186±43及1013±41；组内的平均匹配率分别为91.6%，90.20%对27个差异蛋白质点进行了质谱分析，鉴定出16个蛋白质，在HBV相关性HCC患者PBMC中表达明显增强的有热休克蛋白质27、ras癌基因家族(RAB14)、肌动蛋白、α1-抗胰蛋白及RNA结合蛋白调节亚基等13个蛋白点；而PDX6、HSPA8及锰超氧化物歧化酶在HCC患者PBMC中低表达。

These responses are controlled by a defense signaling network, which is activated by the recognition of a plant resistance gene encoded protein, R protein, and a pathogen avirulence gene encoded protein, Avr protein, in a model of the receptor ligand recognition.

这个信号系统由抗病蛋白和病原菌非毒性蛋白在一种配体受体的互作模式下激发，并由信号分子H2 O2 ，NO和系统信号分子SA ，JA和乙烯和通过关键调控基因传递和放大，最终诱导一系列防卫反应基因的表达和代谢的变化而产生抗性。

The caracter of modified soybean protein plastics by propylol lignin is: it is produced by propylol lignin, soy segregation protein and glycerin. The mass percent of propylol lignin is 1-25, so as to 75-99 of soy segregation protein. The mass of added glycerin is 20-50% of the total mass of propylol lignin, soy segregation protein and glycerin.

羟丙基木质素改性大豆蛋白塑料，其特征在于它主要由羟丙基木质素、大豆分离蛋白和甘油原料制得，羟丙基木质素、大豆分离蛋白各原料所占重量百分比为：羟丙基木质素1-25、大豆分离蛋白75-99，甘油的加入量为羟丙基木质素、大豆分离蛋白以及甘油总重量的20-50％。

The study showed the recombinant wt/mCREG protein depressed the VSMC proliferation depending on dose and the optimal concentration was 400nM;2biologic function of CREG protein and the membrance receptor mechanism:①effect on VSMC migration: the wound healing experiment showed the OB2 cells migration was slower significantly after added wt/mCREG(400Nm) in supernatant. The HITASY cells migration were very slowly and no remarkable change. The gelatinase digestion and Western blot analysis showed the matrix metalloproteinase was decreased and TIMPs was increased;②effect on differentiation: after added wt/mCREG(400nM), the expression of myocardin, SMα-actin, MHC and caldesmin were increased and that of LM-1 and FN were decreased in OB2 cells. These effects were more significant when adding wtCREG.;③effect on VSMC proliferation: Cell cycle assay and BrDU stain showed: after added the wtCREG and mCREG protein, the ratio of cell in G0/G1 phase increased to 0.5773 and 0.5572 from 0.5308 respectively in OB2 group, which increased to 0.7369 and 0.7034 respectively from 0.6297 in HITASY group;3Role of M6P/IGF2R in CREG biologic function:①ELISA and co-immunoprecipitation showed the wt/mCREG binding to M6P/IGF2R directly.②antibody blocking test: when the anti-IGF2R was added to medium at the same time with wt/mCREG at different concentration(2μg/mL、4μg/mL、8μg/mL),the effects of CREG protein which depressing proliferation, migration, secretion and promoting differentiation were blocked, which had the positive correlation to the concentration of added anti body. The studies showed two combinant CREG promoted VSMC switch to differentiation phaenotype, at the same time, depress VSMC proliferation, migration and secreting extracellular matrix.

上述实验结果证实：两种重组CREG蛋白对VSMC增殖均有剂量依赖性的抑制作用，并且相同浓度的糖基化的CREG蛋白对细胞增殖的抑制效应更为显著，最佳效应浓度为400nM；2两种重组CREG蛋白添加后对HITASY和OB2细胞生物学行为的影响：①CREG蛋白对VSMC迁移的影响：刮伤实验发现，加入最佳效应浓度的wtCREG和mCREG蛋白24h后，OB2组迁移能力下降，HITASY组无明显变化；细胞外基质金属蛋白酶-2，9(Matrix metallo-proteinase 2，9，MMP2 ，9)明胶酶电泳检测和Western blot检测结果证实，两种CREG蛋白均可以使OB2细胞合成细胞外基质MMP2，9减少，而组织金属蛋白酶抑制物(Tissue Inhibitors of Metalloproteinases，TIMPs)增加；②CREG蛋白对VSMC分化的影响：加入400nM的wtCREG和mCREG蛋白12h后，OB2细胞myocardin、SMα-actin、MHC、caldesmin表达增加，LM-1、FN表达减少；③流式细胞仪分析细胞周期和BrDU染色分析证实，加入400nM的wtCREG和mCREG蛋白后，OB2组G0/G1期细胞由0.5308分别增加至0.5773和0.5572，HITASY组G0/G1期细胞由0.6297分别增加至0.7369和0.7034；3M6P/IGF2R在重组CREG蛋白的生物学功能中的调控作用：①免疫共沉淀和免疫荧光双染色分析结果显示，CREG蛋白与M6P/IGF2R存在直接结合；②应用抗体阻断实验：将不同浓度的anti- M6P/IGF2R(2、4、8μg /mL)与两种CREG蛋白同时加入培养液中，CREG蛋白抑制VSMC增值、迁移和合成细胞外基质、促进分化的效应减弱，而且与加入anti- M6P/IGF2R浓度正相关。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐