查询词典 protein-free

In this study, we utilized the protein technology to compare the varieties of the sarcoplasmic protein, myofibrillar protein and Urea, SDS extraction of total protein profile in the 5 species of fresh filefish, including Cantherhines dumerilii, Aluterus scriptus, Aluterus monoceros, Monacanthus chinensis and Stephanolepis cirrhifer. Then, these protein profiles were employed to identify the fish species.

本篇论文乃利用SDS-PAGE蛋白质电泳技术分析台湾产主要五种新鲜单棘魨鱼种包括杜氏刺鼻单棘魨、长尾革单棘魨、单角革单棘魨、单棘魨及冠鳞单棘魨之水溶性、盐溶性蛋白质及SDS、Urea萃取之总蛋白质图谱，比较其应用於鱼种鉴别之适切性。

P28 紫色 Formation of a peptide bond 肽键形成–4侧链具有的反应–形成二硫键-其它反应 Tyr,His,Arg Review Isoelectric Point Titration滴定 of an amino acid 滴定 Gly幻灯片 18 His Lys Glu Reaction of amino acids Section 4 Protein isolation and purification 蛋白质分离及提纯 Working With Proteins Experimental techniques for protein analysis and characterization Purification steps A cell contains many types of proteins In the lab we want to isolate a single protein for experiments Purification steps We first grow cells or isolate tissues that contain the protein of interest We break open the cells to produce a crude extract Use centrifugation离心 to separate soluble from insoluble material We fractionate 分离 the protein mixture based on properties of such as size, charge affinity or solubility.

丹磺酰氯与氨基酸反应生成荧光性质强和稳定的磺胺衍生物，用于多肽链NH 用于多肽链 3末端氨基酸的标记烃基化反应(1) 2，4-二硝基氟苯2，4-dinitrofluorobenzene，二硝基氟苯(二硝基氟苯 DNFB也叫做试剂。DNFB在弱碱性溶液也叫做Sanger试剂试剂中与氨基酸发生取代反应，生成黄色化合物二硝基二硝基苯基氨基酸(dinitro phenyl amino acid， DNP氨基酸氨基酸)苯基氨基酸氨基酸(2)苯异硫氰酸酯(phenylisothiocyanate， PITC)在弱碱性条件下，与氨基酸反应在弱碱性条件下，生成苯乙内酰硫脲 PTH衍生物，(phenylthiohydantoin， PTH)衍生物，即PTH-氨基酸，此反应又称之Edman反应，该反应是蛋白质或多肽氨基酸序列测定常用的反应。

Protein in breast milk has 27%α-lactalbumin, and milk α-lactalbumin protein only full 4%, a-whey protein to provide amino acids closest to breast milk composition, protein enhance biological utilization, lower total protein, so as to effectively reduce the burden on the kidneys.

母乳中的蛋白质有27%是α-乳清蛋白，而牛奶中的α-乳清蛋白仅占全部蛋白质的4%，a-乳清蛋白能提供最接近母乳的氨基酸组合，提高蛋白质的生物利用度，降低蛋白质总量，从而有效减轻肾脏负担。

The result showed that : soybean protein and corn protein which were primarily improving the capability of mulsification were selected as the appropriate protein material for protein bar, 10% of saccharose was appended and 20% of moisture was adjusted, perfect protein bar was available by extrusion .

结果表明采用乳化性大豆蛋白和乳化性玉米蛋白为蛋白原料，添加10%的蔗糖和调整含水量为20%，利用挤压膨化可得到较为理想的蛋白棒。

The persent of each component protein can be measured :total protein 52.72 per, albumin 14.36 per, globin 9.30per, titanium 12.87 per , alcoholy protein 2.29per, alkalize protein 2.62per, hawless protein 6.08per.

结果发现：脱脂蝇蛆粉的总蛋白质含量为52.72%，清蛋白14.36%，球蛋白9.30%，小分子蛋白12.87%，醇溶蛋白2.29%，碱溶蛋白2.62%，难溶蛋白6.08%。

The p53 gene structural abnormality and mdm2 protein overexpression might be two factors causing p53 protein overexpresion in NPC. The function of p53 proteins produced by various structural anormality of p53 gene might be different in activating MDM2. The overexpression of p53 or mdm2 protein, particularly both cooperation, might play an important role during NPC genesis.Key words: Nasopharyngeal neoplasms; p53 gene; p53 protein; mdm2 protein

p53基因结构改变和mdm2蛋白过度表达是影响NPCp53蛋白过度表达的两个因素；不同类型p53基因结构改变所产生的p53蛋白激活MDM2的功能并不相同；p53和mdm2蛋白过度表达，尤其两蛋白协同作用，可能在NPC发生中起重要作用。

With the development of molecular biology, based on yeast two-hybrid, many techniques such as yeast one-hybrid, reverse two-hybrid, three-hybrid, and extranuclear two-hybrid were set up to be applied in the research on the interaction between protein-protein, protein-DNA, protein-RNA, and protein-ligand.

随着分子生物学的发展，在酵母双杂交的基础上又建立起了酵母单杂、反向双杂交、三杂交及核外双杂交等多项技术，并已经成功地运用于蛋白质之间、蛋白质与DNA、RNA和配体之间相互作用的研究。

Because of no signal from N-terminal protein sequencing for 43kDa protein directly, a resulting 18kDa peptide from the partial cleavage of 43kDa protein by BrCN had its N-terminal sequences of AFTFKK determined by N-terminal protein sequencing assay. Then, the 43kDa protein was convinced to the enzyme of 3phosphate glycerate kinase (PGK1) by searching YPD.

由于43kDa蛋白直接进行蛋白质氨基端序列测定时没有信号，因而用溴化氰部分化学裂解43kDa蛋白，得到的18 kDa多肽经蛋白质氨基端序列测定，得到其氨基端前6个氨基酸残基序列为AFTFKK，通过计算机检索YPD，确定43 kDa蛋白是3—磷酸甘油酸激酶（PGK1）。

In the second trial, five groups of diets with isonitrigenous (40% protein) and isoenergetic 20 MIJg^(-1 gross energy and different relativity of essential amino acid balance with soybean protein replacing 0.0%, 13.5%, 27.0%, 40.5% and 54% of fish meal protein, were formulated to determine optimal replacement level of soybean protein to fish meal protein.

实验Ⅰ以褐鱼粉为蛋白源，配制5个蛋白水平(31.04%、35.51%、40.89%、46.62%、50.33%)的等能、等必需氨基酸平衡关联度的半精制饲料，探讨翘嘴红鲌对饲料蛋白的需求；经过8周饲养，实验Ⅰ的结果表明，饲料蛋白含量对翘嘴红鲌的增重率、饲料效率和蛋白效率具有显著影响。

Egr-1 mRNA and Egr-1 protein hadn't been found in the normal vein. The expressions of Egr-1 mRNA and Egr-1 protein had biphasic changes. By reverse transcription-PCR and in situ hybridization, we found that the level of Egr-1 mRNA rose at 1 hour after graft, the expression of Egr-1 mRNA was (35±7)%. Decline at hour 6, 24 and day 3, the positive rates of Egr-1 mRNA were (8±2)%,(8±6)% and (8±4)% respectively. Reincrease at day 7, a peak at day 28, the positive rate of Egr-1 mRNA was (45±6)%(compared with other phase, P＜0.01). At day 42, the expression of Egr-1 mRNA declined again. Immunohistochemical staining and Western blot revealed Egr-1 protein had expressed at hour 2 early phase, the expression of Egr-1 protein was (30±5)%, and until to hour 6. The level of Egr-1 protein was decrease at hour 24 and day 3, the positive rates were (7±3)% and (7±8)% respectively.

结果 自体静脉移植后，Egr-1 mRNA和Egr-1蛋白的表达呈双相变化，即移植后1 h， Egr-1 mRNA表达迅速升高，阳性率为（35±7）%，6 h、24 h及3 d时下降到较低水平，阳性率分别为（8±2）%、（8±6）%和（8±4）％，7 d时又再升高，28 d时达高峰，阳性率为（45±6）%，此与其余各时点比较差异均有统计学意义（P＜0.01），42 d时，Egr-1 mRNA的表达再次下降；移植早期（2 h）即有Egr-1蛋白的表达，阳性率为（30±5）%，并持续至6 h，24 h～3 d表达下降到较低水平，阳性率分别为（7±3）%和（7±8）%，7 d时又再升高，至移植后28 d，Egr-1蛋白的表达阳性率达到高峰，为（40±9）%，此与其余各时点比较差异有统计学意义（P＜0.01）。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。