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Our data are showed that apoptosis parameters including DNA fragmentation, nuclear morphological change and sub-G1 fraction, appeared after 24 h by 5 μM rottlerin treatment. Furthermore, rottlerin caused a decrease in 72% of ODC protein expression and an increase in threonine, rather than serine and tyrosine dephosphorylation on ODC enzyme compared with the control after incubation for 10 h. To determine whether ODC could down-regulate rottlerin-induced apoptosis, HL60 cells were stably transfected with ODC cDNA. HL60-ODC cells exhibited a 2.5-fold increase in ODC protein compared with vector only (HL60-pcDNA3) or HL60 cells.

在我们筛选天然化合物实验中,发现rottlerin抑制鸟胺酸去羧化活性最具成效,之前的研究人员已知蛋白激Cd调节鸟胺酸去羧化的活性,因此在人类白血病HL60细胞处理rottlerin的实验中发现加入rottlerin二十四小时后,细胞进行计划性死亡,并可侦测到DNA片段、细胞核浓缩及sub-G1提增;而细胞处理药物十小时后与控制组细胞比较,观察到鸟胺酸去羧化的蛋白表现与活性下降50﹪以上;除此以外,测定鸟胺酸去羧化去磷酸化的情况,发现於酥胺酸位置有显著差异,而丝胺酸和酪胺酸位置并无差异。

Rumex K-1,a new rich-protein green-leaf plant,can yiel d 5 times of protein per unit greater than soybean.

鲁梅克斯k-1酸模,是以鲜叶为主的新型高蛋白植物,单位蛋白产量为大豆的5 倍。

Protein degradation in the rumen is the result of microbial activity and affects by physical and chemical characteristics of protein and the characteristics of rumen and ruminal digesta.

蛋白质在瘤胃内的降解由瘤胃微生物来完成,蛋白质的物理和化学特性、瘤胃及其内容物的特性均影响蛋白质降解。

Results The chimeric protein reacted with anti HBs seral specially.The detection positive ratio of anti HBs is 77.5% and 68%respectively by ELISA and Wstern blot with chimeric protein as coating antigen,the control's was 62%.

结果:嵌和蛋白可与抗 HBs特异性结合,其ELISA检测抗 HBs阳性率为77.5%,Western blot的为68%,而对照试剂盒的为62%。

The expression product was analyzed and its antigenicity was further studied by ELISA and Wstern blot method with chimeric protein as coating antigen.we used chimeric protein as coating antigen to test anti HBs with ELISA and Wstern blot in 58 seral samples.

通过ELISA和Western blot检测表达产物的抗原性,并以表达产物为包被抗原,通过ELISA和Western blot检测58份乙肝患者血清抗 HBs。

Results The chimeric protein reacted with antiHBs seral specially.The detection positive ratio of antiHBs is 77.5% and 68%respectively by ELISA and Wstern blot with chimeric protein as coating antigen,the control's was 62%.

结果:嵌和蛋白可与抗HBs特异性结合,其ELISA检测抗HBs阳性率为77.5%,Western blot的为68%,而对照试剂盒的为62%。

Developments of high through-put experimental techniques and in silico prediction methods help to increase direct and indirect protein-protein interactions data.

高通量技术的应用结合计算机预测方法的发展,使得直接和间接来源的蛋白质相互作用数据得到了大规模的增加。

Among those, the phylogenetic profiles method, one of popular in silico proteomics methods, has been widely used to predict protein-protein interactions.

这些方法之中,系统发生谱的方法,是一个比较受欢迎的'in silico'的蛋白质组预测方法,现已广泛用于蛋白质相互作用预测。

Therefore, we characterized the Cyt c P71H/M80V variant protein electrochemically and spectroscopically. All the experimental results showed that the introduced histidine at position 71 did not act as a functional distal-histidine, but most likely coordinated to the heme iron, maybe due to the flexibility of theΩloop segment (resides 70-84) of Cyt c protein.

于是我们对P71H/M80V蛋白性质进行了一系列的研究,结果表明可能是由于Cyt c中Ωloop D肽段(residues 70-84)具有很强的柔韧性,使得His71并没有象HRP中的distal histidine一样起到酸碱催化功能,而是与heme发生了配位作用,无论是在氧化态还是还原态都成了heme的第六轴向配体。

Base on the comparison of different feeding effect of dietary starch source, starch in vivo digestion and absorption as well as it's effect on energy and protein utilization, and fractional protein synthesis rate of splanchnic tissues in weaned pigs were further studied.

在比较不同来源饲料淀粉饲养效果差异的基础上,进一步探讨不同饲料淀粉在断奶仔猪小肠不同部位的消化吸收及对能量、蛋白质利用和内脏组织蛋白质合成率的影响,从消化道和组织利用两个层次解释不同饲料淀粉影响仔猪生长的原因。

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It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发,分析对称连杆曲线上鲍尔点的产生条件,提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品,年生产总值3000万美元,产品价廉物美、选料上乘、质量保证,深受国内外客户的青睐

The eˉtiology of hemospermia is complicate,but almost of hemospermia are benign.

血精的原因很,以良性病变为主。