查询词典 protein shock

There have been massive researches on improving the properties of protein in recent years, this article emphatically begin with the interaction between tea polyphenol and protein (egg albumen protein, soybean protein isolated, whey protein concentrated, bovine serum albumen), study their functional rules and that under the changed food processing environment (the pH value, salt ion concentration, sucrose density), and then apply the interaction rules into two different kinds of cakes for improving the technology of their production, aiming for provide the new mentality and method on improving the properties of protein as well as speed up the application of tea polyphenol.

本文着重从茶多酚与蛋白质的相互作用着手，研究了茶多酚及其主要单体组分对蛋清蛋白、大豆分离蛋白、乳清浓缩蛋白及牛血清蛋白的功能特性的影响规律，同时也探讨了环境因素(pH值、盐离子浓度、蔗糖浓度)与茶多酚协同作用对蛋白质这些功能特性的影响规律，并且初步应用了这种相互作用规律到两种不同工艺蛋糕的生产中来，目的是为蛋白质功能特性的改善提供新的思路和方法以及加快茶多酚在食品中的应用。

objective to investigate staining methods for two-dimensional gel(2-de)electrophoresis in multidrug resistance of gastric cancer.methods cultured vincristine-resistant human gastric cancer cell line sgc7901/vcr and its parental cell line sgc7901.variant amount protein of those cells were separated by 2-de.gels were stained with silver nitrate or colloidal coomassie brilliant blue,and scanned by image scanner.results well-resolved,reproducible 2-de patterns of sgc7901/vcr and sgc7901 were established.silver staining was better when protein sample amount was low,overloaded protein will interfere resolution of the maps.gels stained with colloidal coomassie brilliant blue had more protein spots numbers and abundance without apparent trails when increased loading protein sample.conclusion two staining methods were influenced largely by the sum of protein samples,properly selection may be helpful for further study with proteomics in multidrug resistance of gastric cancer.

低甲氧基果胶的胶凝机理及防止预凝胶。。。他扎罗汀凝胶与克林霉素凝胶治疗痤疮。。。注射隆乳后经乳晕切口取出聚丙烯酰胺。。。目的分析利用蛋白质组学方法研究胃癌耐药相关蛋白质中双向电泳凝胶的染色显示。方法培养胃癌细胞sgc7901和长春新碱诱导的耐药胃癌细胞sgc7901/vcr，用双向凝胶电泳技术分离总蛋白，银染及胶体考马斯亮蓝染色，image scanner扫描仪扫描凝胶。结果获得了背景清晰、重复性好的双向凝胶电泳图谱，两种染色凝胶相比，硝酸银染色在样品少时显示更佳，过量则影响图像质量，而胶体考马斯亮蓝染色在上样量增加时的凝胶蛋白质点数目及丰度均增加，并无明显拖尾。结论两种显色方法受样品量影响较大，恰当选用有利于通过蛋白质组学研究胃癌耐药机制工作的进一步开展。

Heat shock protein 70 (HSP70) is an important member of heat shock protein family and play an important role in animal cell growth, development, and prolificacy, gene expression and protein synthesis, folding and transportation.

热休克蛋白(Heat shock protein， HSP)是指细胞在反应刺激原的诱导下所生成的一组蛋白质，特别是环境高温的诱导。

Methods Th multi-epitope peptide was made up of 5 Th epitopes(heat shock protein 65 of Mycobacterium tuberculosis p1-20,E2 protein of Rubella virus p54-65,engineering epitope PADRE,heat shock protein 60 of Chlamydia trachomatis p35-48,and tetanus toxin p830-843),and its gene sequence including of 219bp.

方法复合T辅助细胞表位由5个T辅助细胞表位组成(结核杆菌热休克蛋白65p120、风疹蛋白E2-4p5465、人工合成T辅助细胞表位PADRE、沙眼衣原体热休克蛋白60p3548、破伤风类毒素p830843)，其基因序列由219个核苷酸组成。

Compared with the initial value problems of scalar conservation laws with smooth flux function, the global weak entropy solutions for the initial-boundary value problems of scalar conservation laws with weak discontinuous flux function include the following new interaction types: a rarefaction wave collides with the boundary and is absorbed compltetely or partially by the boundary; a rarefaction wave collides with the boundary and the boundary will reflect a contact or non-contact shock wave; a contact or non-contact shock wave collides with the boundary and is absorbed by the boundary; a contact or non-contact shock wave collides with the boundary and a new non-contact shock will rebound from the boundary simultaneously or later.

与具有光滑流函数的单个守恒律的初始值问题相比，具有弱间断流函数的单个守恒律初边值问题的整体弱熵解中包括下列新的相互作用类型：稀疏波碰到边界并被边界部分或全部吸收；稀疏波与边界相撞，边界反射出一个接触或非接触激波；接触或非接触激波碰到边界并被边界吸收；接触或非接触激波与边界相撞，边界同时或稍后反射出一个新的非接触激波。

The usual way to calculate the residual shock response spectrum of the base shock response is as follows: the time domain shock response of the base is calculated firstly, then the residual shock response spectrum of the base response is calculated.

冲击作用下设备安装基座响应的冲击余谱的一般计算方法是先计算冲击作用下设备安装基座的时域响应，再计算安装基座响应的冲击余谱。

To obtain a complete picture about shock formation in non-viscous flow, in the succeeding chapter we investigate the isothermal shocks in adiabatic flows. We find that in this case the shock solution still exists, but the structure and the properties of the solution are greatly different from above even qualitatively. In some region of the parameter space there only exists unstable shock solution and in some region of the parameter space there may exist two stable shock solutions.

为得到完整的无粘流体中激波形成的图象，在接下来的第四章，我们研究绝热流体中等温作为激波条件时的情况，发现此时激波解仍存在，但解的结构和性质甚至在定性上都与前两章有了显著的不同：某些参数空间只存在非稳定的激波解，某些参数空间则会同时出现两个稳定的激波解。

The calculated results indicate that a bifurcated A. wave can be formed when the shock passes through the flame, and pre-induced shock transmission in the flame leads to the formation of Mach stem, followed by spheral shock wave. The Rayleigh-Taylor and Kelvin-Helmholtz instabilities occurred in the flame induced by shock wave lead to the flame distortion and breakup.

计算结果表明，激波掠过火团时，分叉形成λ波；前引激波在火团内透射并在轴线处碰撞形成Mach杆，进而逐渐发展为球形激波；火焰在激波作用下先后出现Richtmyer-Meshkov不稳定和Helmholtz不稳定，火焰逐渐变形以致破碎；燃烧速率因此剧增。

However,it is the dissipa-tive mechanism,i.e.the mechanism of energy localization that attracts one′sattention for the problem of shock initiation.Since the test result of shock initia-tion about fine-grain explosive charge does not support hot spot ignition model,the author of this dissertation advances that the mechanism of energy localiza-tion is coincident with phonon-fracton crossover of fractal network for ultra-fine-grained charge and,moreover,analyzes the relation between shock sensi-tivity and porosity,which is consistent with shock Hugoniot curve for porousmaterials.

由于超细颗粒压装炸药的冲击起爆实验不支持热点点火模型，为此、本文提出对于单晶颗粒半径为微米以下量级的药床，冲击起爆的能量局域化机制与分形网络中弱局域化声子向强局域化分形子的渡越对应，并且根据这一机制分析了超细颗粒药床的冲击感度和孔隙度的关系，结果与多孔物质冲击压缩的P-V图吻合。

RESULTS: A well-resolved and reproducible 2-DE pattern of PBMC was obtained from patients with HBV associated HCC or LC. For HCC, the mean number of protein spots was 1186±43, with an average matching rate of 91.6%, and for LC, the mean number of protein spots was 1013±41, with an average matching rate of 90.2%. Twenty-seven differential proteins were tested by MALDI-TOFMS, and 16 were identified. Of the 16 proteins, 13 were up-regulated in patients with HCC, including heat-shock protein 27, member RAS oncogene family (RAB14), actin, alpha 1 antitrypsin, RNAbinding protein regulatory subunit and so on. By contrast, the levels of proteins such as PDX6, HSPA8 and MnSOD were significantly reduced in HCC patients.

结果：得到了分辨率较高、重复性较好的HBV相关性HCC及LC患者PBMC双向凝胶电泳图谱，HBV相关性HCC及LC患者PBMC凝胶的平均蛋白质点数分别为1186±43及1013±41；组内的平均匹配率分别为91.6%，90.20%对27个差异蛋白质点进行了质谱分析，鉴定出16个蛋白质，在HBV相关性HCC患者PBMC中表达明显增强的有热休克蛋白质27、ras癌基因家族(RAB14)、肌动蛋白、α1-抗胰蛋白及RNA结合蛋白调节亚基等13个蛋白点；而PDX6、HSPA8及锰超氧化物歧化酶在HCC患者PBMC中低表达。

You can do some assertiveness training.

你可以进行一些自信训练。

We were well on the path to making a rear-wheel-drive global platform," says Mays."

我们正致力于建立一个后轮驱动的平台，"Mays这样说道。"