查询词典 protein shock

SDS-PAGE results showed that as to Mut+ recombinant highest yield was obtained after 4 days inducing and with the culturetime prolonged it reduced. Pokeweed antiviral protein gene expressed well when methanol concentration reached 10g/L. Pokeweed antiviral protein obtained high yield in thin acidic culture medium (pH6.0-6.4) and its quantity in total mass of secrete protein exceeded 30%.

SDS-PAGE分析结果表明，Mut~+组菌株在甲醇诱导第四天后PAP在培养液中积累量达到最高水平，延长培养时间会导致产量下降；在10g/L的甲醇浓度诱导下，PAP的表达量达到最高；培养基pH值在偏酸性条件下(6.0-6.4)PAP的表达量都维持在较高的水平。

However, none of current molecular mechanical force fields can exactly treat the polarization effects in protein-protein and protein-ligand binding, even for polarizable force fields.

然而，目前还没有任何一种分子力学力场能够处理蛋白质和蛋白质或者蛋白质和配体之间的极化效应。

IGF-1 concentration in serum was measured with RIA. Protein concentration in urine was measured with ELASE. Urinary protein excreted in one day was measured with ponceau S. Total protein and albumin level in serum were measured with automatic biochemistry appliance.

以放射免疫法测血清IGF-1水平，用酶联免疫吸附试验测尿微量白蛋白浓度，S-丽春红测24h尿蛋白定量，全自动生化仪测量血清总蛋白和白蛋白。

HCV NS3C/NS4 protein fused with E. coli thioredoxin was expressed insolubly although thioredoxin is a highly soluble fusion partner. NS5B-dc21 protein was soluble, which is a truncated version of NS5B, while NS5AB protein was insoluble, which needed resolubilization and refold procedures.

HCV NS3C/NS4蛋白是以硫氧还蛋白融合表达的方式获得的，尽管硫氧还蛋白有很强的助溶作用，该蛋白依然不可溶形式存在；NS5B—dc21蛋白通过C末端疏水序列的删除，获得了可溶表达；NS5AB蛋白以不可溶形式表达，需要复性。

We extracted membrane protein of Mycoplasma hyopneumoniae MY-99 strain from its different passages strains MY-99l,MY-993 and rejuvenescence strain MY-992, then analyzed them by SDS-PAGE, the results show that the membrane protein of Mycoplasma hyopneumoniae MY-99 strain mutated during artificial culturing . According to the electrophoretic profiles, the membrane proteins of MY-991 include 7 protein bands: 94.4KD,57.5KD,46.0KD,36.0KD,32.4KD,20.4KD and 16.6KD.

采用0.4％TritonX-100结合KI提取猪肿炎支原体MY-99株不同代次的传代株MY-991、MY-993和复壮株MY-992的膜蛋白，并通过SDS-PAGE技术对其膜蛋白进行比较分析发现，在人工培养条件下猪肺炎支原体MY-99株的膜蛋白发生了一定的变异，其中MY-991株的膜蛋白有七条蛋白条带：94.4KD、57.5KD、46.0KD、36.0KD、32.4KD、20.4KD和16.6KD，MY-993株的膜蛋白有七条蛋白条带：94.4KD、57.5KD、46.0KD、41.0KD、36.0KD、32.4KD和19.5KD，MY-992株的膜蛋白只有两条蛋白条带：46.0KD和32.4KD。

With the advent of post-genomic era, identification of protein-protein interaction has become another hot spot of protein research and promoted the invention, development and complement of relative techniques.

随着后基因组时代的到来，阐明蛋白质间相互作用关系成为蛋白质研究的又一热点，促进了相关技术的不断产生、发展和完善。

The synthesized cDNA is a continuous band less than 6. 0kb shown on x-film through α〓PdCTP which was inserted in the first strain of cDNA. The titer of the Ha-cDNA library was about 1. 5×10〓cfu/ml which could represent all protein-encoding sequences from Hz-AM1 cells; Namely, the cDNA library has representativity. After the successful construction of the Ha-cDNA library, the open-reading frame of vp39 gene of Heliothis armigera was then fused in frame with the Ga14-DNA-binding domain in the pGBT9 plasmid and the fusion protein Ga14-VP39 was expressed in yeast to be used as bait protein.

在成功构建棉铃虫细胞Hz-AM1cDNA文库的基础上，本文接着将棉铃虫核型多角体病毒衣壳蛋白VP39（HaNPV-VP39）基因克隆到酵母双杂交系统(yeasttwo-hybrid system)中的质粒pGBT9上。pGBT9上含有酵母菌转录激活因子GAL4 DNA结合域的编码区（GAL4-DBD），使克隆到质粒pGBT9上的HaNPV-vp39基因与GAL4-DBD基因融合，在酵母菌中表达DBD-vp39融合蛋白，该融合蛋白即为酵母双杂交系统中的诱饵蛋白。

Its current projects include: quantitative prediction of protein-protein interactions and membership in protein complexes; improved identification of peptide sequence; development of a Bayesian statistical framework for assessing and representing uncertainty in gene function annotation etc.

其最近的研究项目包括：蛋白质复合体中蛋白质-蛋白质相互作用及其成员的定量预测；经改良的肽序列鉴定方法；贝叶斯统计方法在基因功能评价和不确定性分析等方面应用的发展等。

Containing many T cell epi-positions and neutralizing antibody epi-positions, the core protein region and envelope protein 2 of Hepatitis C virus structural protein may induce protective immunity responsion in body.

丙型肝炎病毒(hepatitis C virus，HCV)结构蛋白的核心蛋白和包膜蛋白2(E2)含有多个T细胞表位和中和抗体表位，可诱导机体产生保护性免疫应答。

This is because the alfalfa products can be a variety of amino acid balance of ruminant livestock, and provide high-quality vitamins and fiber, so that rumen protein to total protein content of 58% to 60%, thus significantly increasing the efficiency of alfalfa protein utilization results.

这是因为苜蓿草产品可平衡反刍家畜的各种氨基酸，并提供维生素和高品质纤维素，使瘤胃蛋白含量达到总蛋白质的58％～60％，从而大大提高苜蓿蛋白质利用效率的结果。

You can do some assertiveness training.

你可以进行一些自信训练。

We were well on the path to making a rear-wheel-drive global platform," says Mays."

我们正致力于建立一个后轮驱动的平台，"Mays这样说道。"