查询词典 protein crystal

The objectives of this study are:(1) To amplify Tb wbbL gene encoding rhamnosyl transferase bypolymerase chain reaction from the genomic DNA ofM.tuberculosis H37RV strain;(2) To clone PCR product of Tb wbbLgene into a cloning vector pMD18-T for sequencing;(3) To subcloneTb wbbL gene to an expression vector pET16b to construct pET16b-Tb wbbL; and to overexpress Tb WbbL protein in E.coli BL21(DE3)under different induction conditions;(4) To establish theco-expression system for expressing chaperons of pKJE7 plasmidand soluble Tb WbbL protein in E.coli BL21(DE3) under differentinduction conditions;(5) To test expressed WbbL protein by SDS-PAGEand Western blot methods.

本论文的目的是：(1)利用 PCR 方法从结核分枝杆菌 H37Rv 菌株的基因组DNA 中扩增出 Tb wbbL 基因；(2)将 Tb wbbL 基因克隆到pMD18-T 克隆载体中，经 DNA 序列测定证实为正确的基因；(3)将 Tb wbbL 基因亚克隆到 pET16b 表达载体中并通过改变不同的诱导条件在大肠杆菌 BL21(DE3)中表达 Tb WbbL 蛋白质；(4)建立在 BL21(DE3)大肠杆菌中共表达 Tb WbbL 蛋白质与分子伴侣的体系，优化表达条件以高效表达出可溶性 Tb WbbL蛋白质；(5)用 Western blot 方法鉴定所表达的 Tb WbbL 蛋白质为 Tb wbbL 基因产物。

In this experiment, a new method was used to purify the toxin protein produced by Verticillium dahliae. The supernatant of fungus culture was frozen and dried with Lyophilizer first, and then dialysed by Dialysis Membranes (MWCO 1000) after dissolved in distilled water. This method can eliminate the salt and sucrose in culture medium and reserve the protein component almost completely. VD-toxin were analysed using sodium dodecyl sulfate polyacrylamide gel analysis. The results indicated that the protein components were very complex, and included glycoprotein within 35.8kDa-83.2kDa. Furthermore, treatments on glycoproteins with high temperature, conA and proteinase alleviated, not abolished, their activities.NO and H_2O_2 production were assayed in two cultivars of cotton cells which were treated with VD-toxin.

本实验首先确定了用冷冻浓缩后透析的方法初步提纯棉花黄萎病菌毒素，该方法能有效去除粗提毒素中的蔗糖和盐离子并能最大程度的保留黄萎病菌分泌的蛋白成分；对毒素蛋白成分鉴定结果表明蛋白条带多，每种蛋白的含量少，小分子量蛋白含量多，糖蛋白染色结果显示，毒素蛋白中分子量在35.8kDa-83.2kDa之间的蛋白大多数为糖蛋白，分子量小于35.8kDa的蛋白多为非糖蛋白；性质鉴定结果显示，毒素中有活性的蛋白成分具有部分可耐高温高压，且毒素蛋白中的蛋白成分和糖基成分都具有致萎活性。

In one embodiment of the invention, anion exchange chromatography of the vegetarian Protein A media produces a vegetarian Protein A eluant that contains an at least 95% pure Protein A.

在本发明的一个实施方案中，植物蛋白A培养基的阴离子交换色谱产生含有至少95％纯蛋白A的植物蛋白A洗脱液。

The seed embryoes were penetrated by 〓 ion beams of 75 MeV/u and the seed endosperms were implanted by the proper degraded 〓 ions, and soluble protein of the seedling in M1 generation was divided into 5 sections to analyze their changes at different molecular weight. 1 In comparison with control, the relative content of the second section (molecular weight is heavier in all sections) of all irradiated materials got down, while the relative content of the fifth section (molecular weight is the lightest) of those went up. 2 The penetrating seeds made the relative content of the first section (molecular weight is the heaviest) and the third section (mid-molecular weight) drop, while the fourth section went up. Moreover, the changes of the third section and the fourth section of the penetrated materials were clearly different from those of the implanted materials. 3 The difference between implanted embryo and implanted endosperm existed in deeper dropping of the first section and higher rising of the fifth section on relative content of soluble protein of the latter. 4 On irradiated materials at lower doses, there were exceptional changes of soluble protein, it may be thought that there was excited effect of low dose.

1.75MeV／u 〓O〓离子经过适当降能后注入小麦种子的不同部位和贯穿整个种子，对其M1代幼苗的可溶性蛋白质组分以及电泳中不同区段蛋白质组分相对含量加以分析，结果表明：1）同对照相比，随着辐照剂量的增加，所有辐照材料第二区段蛋白质组分的相对含量下降；而第五区段蛋白质组分的相对含量升高。2）种子的贯穿处理，同时也引起第一区段和第三区段蛋白质组分相对含量的下降以及第四区段的升高；其中第三、四区段的变化明显区别于注入效应。3）注入胚和胚乳的区别在于后者第一区段蛋白质组分相对含量的较大下降和第五区段的较大提高。4）小剂量辐照的材料，可溶性蛋白质组分的变化异常，可能与低剂量辐射兴奋效应有关。

Protein phosphorylation catalysed by protein kinase is the predominant form of signaling in both procaryotes and eucaryotes, Protein Ser/Thr and Tyr kinase play essential roles in signal transduction.

蛋白质激酶催化的蛋白质磷酸化是原核和真核生物中一种主要的信号传导方式，蛋白质Ser／Thr和Tyr激酶在信号传导过程中起着必需的作用。1993年，张承才在Anabaena sp。

The results have shown that for fodders containing different protein levels, the excretion of nitrogen and phosphor was the lowest (34.05%) when the crude protein reached 30%,and the excretion increased gradually otherwise;and for fodders containing different protein qualities,the excretion of nitrogen was the highest,fed with fish meal.

因此测定鱼类氮、磷排泄对研究养殖鱼类对氮、磷的日粮需要和养殖水域环境污染具有重要意义。许多学者从事了这一研究。草鱼作为我国重要经济养殖鱼类，有着较大的养殖面积。草鱼氮、磷排泄情况的研究在国内还未见报道，在国外，C.G。

The results have shown that for fodders containing different protein levels, the excretion of nitrogen and phosphor was the lowest (34.05%) when the crude protein reached 30%,and the excretion increased gradually otherwise;and for fodders containing different protein qualities,the excretion of nitrogen was the highest,fed with fis...

当粗蛋白水平为30%时，氮、磷排泄率最低(34.05%)，粗蛋白高于或低于此值时，氮、磷排泄率呈增加趋势；对不同蛋白质量组：鱼粉组氮排泄率最高，而磷排泄率在各组无显著差异。

It develops than amino acid and protein are easier to digest beneficial within the human body fungus physiology activity and growty such as absorbing,promoting the lactobacillus,pair ofbranch bacilli;Be used for formula milk powder and protein drink being to be able to improve the product protein contents,can increase the product nutrition function factor.

它比氨基酸和蛋白质更易于消化吸收，促进乳酸菌，双岐杆菌等人体内有益菌类生理活性和生长发育；用于配方奶粉和蛋白饮料即能提高产品的蛋白含量，又能增加产品的营养功能因子。

For all of GAS toxicity factors, M protein is the most important toxicity factor. M protein is a membrane protein which have α-helical coiled coil structure on the plasma membrane. It helps GAS to attack the host and have resistance to the immune system.

在A型链球菌所有的毒性因子中，M蛋白是最重要的毒性因子。M蛋白是A型链球菌上的一种膜蛋白，在A型链球菌细胞膜上形成α-helical coiled coil结构。M蛋白帮助A型链球菌侵入宿主并且逃避宿主的免疫系统攻击。

Through optimization of thick mash fermentation conditions, suitable fermentation conditions for improving solubility of corn protein were determined, so as to improve the solubility of corn protein favorable for digestion of protein by animals, so that the utilization rate of the corn gluten meal as a feed could be increased.

通过甘浓醪发酵条件的优化研究，确定适宜的改善玉米蛋白溶解性的发酵条件，提高玉米蛋白的溶解性，有利于动物对蛋白的消化吸收，从而提高玉米蛋白作为饲料的利用率。

Nowadays, most of research are to build a transmutative Petri Nets through adding controlling place sets, controlling arc sets and controlling policy to the basic Petri Nets, while the Controlled Petri Nets could be used to argue many controlling theory problems conveniently and to induce many logically and physically supervisory and solve the Event Feedback Controlling Problems and State Feedback Controlling Problem in DEDS supervisory theory.

目前大多数的研究表现为在变形后的受控Petri网基础上，利用各种方法求得各种逻辑型、结构型控制器，解决DEDS监控理论中的事件反馈控制问题与状态反馈控制问题。

On one hand, there are discussions with the works council and union about extension of short time working up to the end of September.

一方面，有讨论，工程理事会和联盟关于延长工作时间短至9月底。