查询词典 protein crystal

In this study, oral tolerance was induced by tube-feeding transgenic mice with 0.5mg/day OVA protein daily, simultaneously sensitized by OVA protein with aluminum hydroxide. Oral-administered OVA protein (0.5mg/day for 5 or 10 consecutive days) resulted in significant lowered airway hypersensitivity as well as reduced inflammative cells infiltration, titer of serum OVA-specific IgE, IL-5 secretion in bronchoalveolar lavage fluid, and IL-4 secretion in splenocyte.

首先，本实验於小鼠中成功诱发了呼吸道过敏、大量OVA特异性IgE抗体生成、及嗜酸性白血球不正常聚集等现象；致敏前，同以连续管餵小鼠OVA抗原(0.5mg/day) 5天或10天后，结果显示皆能缓和呼吸道阻力、减低肺部发炎细胞浸润现象、降低血清中OVA特异性IgE抗体的生成量、也减少肺部冲洗液及脾脏细胞内第二型辅助T细胞(type II helper T cell， Th2)细胞激素的分泌(IL-4、IL-5)。

The proportions of Ⅰ~Ⅱgrade and Ⅲ grade were 73.8%、 83.1%; 45.7%、 65.2% between 92 samples of Urgur patients and 188 samples of han patients . Lymphy node metastasis in Uygur patients were greater than Han patients in Ⅰ~Ⅱgrade and Ⅲ grade (X~2=13.14, P.001; X~2=5.08, P.05).(3) The proportions of the expression of VEGF-AmRNA and its protein were 31.8%、 57.1%、64.3%, 27.3%、 53.6%、 59.5% in difference pathological grade in 92 samples, With increasing of the pathological grade,the VEGF-AmRNA and its protein expression are highly (X~2=6.26, P.05; X~2=6.21, P.05); The proportions of the positive expression of VEGF-AmRNA and its protein were 68.4

392例浸润性乳腺癌组织中VEGF-AmRNA和其蛋白的表达在不同组织学分级中其阳性率分别是31.8%、57.1%、64.3%，27.3%、53.6%、59.5%；二者在不同组织学分级中表达差异具有统计学意义(X~2=6.26，P.05；X~2=6.21，P.05)；在淋巴结转移组与非转移组中VEGF-AmRNA和其蛋白的表达阳性率的分别是68.4%、31.49，61.4%、31.4%，在淋巴结转移组VEGF-AmRNA和蛋白的明显高于淋巴结未转移组(X~2=11.96，P.001；X~2=7.79，P.05)；VEGF-AmRNA和其蛋白的表达

Results Although overexpression of P53 protein was not detected in normal eyelid cutaneous epithelium, meibomian gland and mild epithelial dysplasia, the positive rate of P53 increased in degree in moderate and severe dysplasia, carcinoma in situ and invasive tumors, and PCNA labeling index progressively increased too. Overexpression of P53 protein was detected in 30/53 cases of basal cell carcinoma, 16/32 cases of squamous cell carcinoma, 10/17 cases of meibomian gland carcinoma, and the intensity of P53 protein immunostaining increased with the lowering in the degree of histological differentiation.

结果 在正常眼睑皮肤、睑板腺及轻度不典型增生眼睑上皮中，未见P53蛋白表达，而中、重度不典型增生，原位癌，浸润癌中，P53蛋白阳性表达率逐渐增高，其 PCNA指数也有增加趋势。30/53例基底细胞癌(basal cell carcinoma，BCC)、16/32例鳞状细胞癌（squamous cell carcinoma，SCC）、10/17例睑板腺癌中，P53蛋白阳性表达；同时，随着肿瘤组织分化程度的降低，P53蛋白阳性表达程度有逐渐增高的趋势。

Results: The expression of CTGF protein and CTGF mRNA in the nomal and sham operation group is low in the intracytoplasm of Mesangial cells, renaltubular epithelial cells and renal interstitial cells. Compare with sham operation group, the expression of CTGF protein and CTGF mRNA as yellow particulate in the mode group was markedly increased, and the fibrosis of nephridial tissue is obvious. Panaxnotoginseng could significantly decrease the expression of CTGF protein and CTGF mRNA(P.05), and there was no significant difference between Panaxnotoginseng treatment group and Benazepril treatment group.

结果：正常组及假手术组肾小球系膜细胞、肾小管上皮细胞以及肾间质细胞胞浆内有少量CTGF蛋白及其mRNA的黄色颗粒状阳性反应物，模型组上述部位的CTGF蛋白及其mRNA呈强阳性表达，三七总皂苷组上述指标表达较模型组显著减少，有统计学差异(P.05)，与阳性药物组比较差异不显著。

Recombinatino polypeptide or chemically synthesized polypeptide antigen is used to immunize the mammal to obtain polyclonal antibody and the nonconservative amion acid sequence of the antiboby to N-terminal with recognition site qBrn-2 of development regulatory protein qBrn-2. The antibody does not recognize other POU structure domain protein, and recognizes specifically qunique protein in Western imprinting experiment. The qBrn-2 expression mode obtained immunohistochemistry process is identical with the resulted height of in-situ molecular hydridization.

以重组多肽或化学合成多肽为抗原免疫哺乳动物获得的多克隆抗体，这一抗体对发育调控蛋白qBrn－2的识别位点为qBrn－2的N－末端的非保守性的氨基酸序列，该抗体不识别其它POU结构域蛋白；在Western印记实验中，该抗体特异地识别单一的蛋白；利用免疫组织化学接示的qBrn－2表达模式与分子原位杂交的结果高度一致。

Using ant colony algorithm, we make an alignment between the orphancy protein sequence and the protein sequence of clustering center to predict function of orphancy protein.

提出了利用蚁群算法双序列比对从蛋白质相互作用网络中预测孤立蛋白质与聚类中心蛋白质的相互作用，进而预测孤立蛋白质的功能。

The number and location of protein bands were different among these species. It was also obvious that the positions of the main protein bands in Blattella germarica were different from those in two Periplaneta species, and the positions of the main protein bands in female insect were different from those in male insect.

通过比较发现3种蜚蠊的蛋白质区带数目和位置有差别；德国小蠊主要蛋白质区带的位置与两种大蠊相差明显；♀♂之间也有差别。

Comparison from the Germany isolate, phocine distemper virus type Ⅱ(PDV-2) and vaccine strain, the major difference is the long signal peptide domain while the mature protein exhibit high identity, and all of the 13 serine residues, four potential asparagine glycosylation sites and two hydrophobic regions supposed to affect the fusion function are completely identical. These data supports the view that F protein is more conservative than H protein in CDV.

三、犬瘟热病毒中国分离株囊膜糖蛋白基因免疫小鼠诱发的抗体应答经一系列步骤将CDV-YZ0101株的两囊膜糖蛋白基因定向导入真核表达载体pcDNA3.1中，DNA测序和酶切分析筛选阳性重组质粒克隆pcDNA-H和pcDNA-F，以重组质粒DNA和脂质体共转染COS-7细胞，用间接免疫荧光试验证实转染的COS-7细胞的胞浆中分别表达了CDV的H和F蛋白。

MTT assay FAK signaling pathway inhibitor genistein on human corneal epithelial cell cytotoxicity;RT-PCR detection of human corneal epithelial cells adhesion to fungus at different times,extracellular matrix protein including laminin,fibronectin,FN glass,Ⅳcollagen,transmembrane protein integrinαⅤ,integrinβ1(ITGβ1),as well as the FAK signaling pathway FAK1,FAK2 and Paxillin gene expression;Western blot detection of the signal transduction pathway adhesion-associated protein ITGβ1,FAK and PAX expression and the inhibition of genistein. Immunocytochemical method was used to observe the LN,FN and FAK expression in human corneal epithelial cells during interaction with the fungues;Laser scanning confocal microscope had a cell positioning on FN,FAK and PAX,observed the changing of the human corneal epithelial cytoskeleton after stimulated by fungues;Quantitatived by flow cytometry to detect of human corneal epithelial cells with PAX at ITGβ1 fungal expression after adhesion;Optical microscopy quantitied the fungues and human corneal epithelial cell adhesion and recorded to determination the integral optical density afrer adhesion;Scanning and transmitted electron microscope observed the changing of cell ultrastructure after fungues and human corneal epithelial cell adhesion.

第一部分真菌激活人角膜上皮细胞FAK信号转导通路的体外实验研究将三种常见致病真菌(白色念珠菌、烟曲霉菌和茄病镰刀菌)分别与人角膜上皮细胞共孵育，MTT法检测FAK信号通路抑制剂染料木黄酮的对人角膜上皮细胞的细胞毒性作用；RT-PCR检测真菌黏附人角膜上皮细胞后不同时间细胞外基质层连蛋白、纤连蛋白、玻连蛋白、Ⅳ型胶原、跨膜蛋白整合素αV、整合素β1(ITGβ1)，以及FAK信号通路中FAK1、FAK2和桩蛋白基因的表达情况；Western blot的方法检测黏附信号转导途径相关蛋白ITGβ1、FAK和PAX的表达，以及染料木黄酮对真菌刺激人角膜上皮细胞FAK信息通路活化的抑制作用；免疫细胞化学方法观察人角膜上皮细胞与真菌相互作用过程中LN、FN和FAK的表达；激光共聚焦显微镜对FN、FAK和PAX进行了细胞定位，并观察真菌刺激后人角膜上皮细胞骨架的变化；流式细胞仪定量检测人角膜上皮细胞ITGβ1与PAX在真菌黏附后表达的改变；光学显微镜观察真菌与人角膜上皮细胞黏附数量，记录并测定了黏附后积分光密度值OD扫描及投射电镜观察了真菌与人角膜上皮细胞黏附后，细胞超微结构的改变。

Inhibition of PKCdelta by rottlerin or knockdown of TG2 protein by a TG2-specific siRNA resulted in a marked increase in autophagy shown by presence of autophagic vacuoles in the cytoplasm, formation of the acidic vesicular organelles, membrane association of microtubule-associated protein 1 light chain 3 (LC3) with autophagosomes, and a marked induction of LC3-II protein, important hallmarks of autophagy, and by electron microscopy.

抑制PKCdelta的rottlerin或击倒的TG2蛋白质的TG2特定的siRNA导致显着增加，表明自噬存在自泡在细胞质，形成酸性囊泡细胞，膜协会微管相关蛋白1轻链3（ LC3 ）与autophagosomes ，并显着诱导LC3 -Ⅱ蛋白的重要标志吞噬，并通过电子显微镜。

Nowadays, most of research are to build a transmutative Petri Nets through adding controlling place sets, controlling arc sets and controlling policy to the basic Petri Nets, while the Controlled Petri Nets could be used to argue many controlling theory problems conveniently and to induce many logically and physically supervisory and solve the Event Feedback Controlling Problems and State Feedback Controlling Problem in DEDS supervisory theory.

目前大多数的研究表现为在变形后的受控Petri网基础上，利用各种方法求得各种逻辑型、结构型控制器，解决DEDS监控理论中的事件反馈控制问题与状态反馈控制问题。

On one hand, there are discussions with the works council and union about extension of short time working up to the end of September.

一方面，有讨论，工程理事会和联盟关于延长工作时间短至9月底。