查询词典 protein crystal

In this paper, we studied M. meretrix ferritin, cathepsin B and caspase genes, which are involved in clam larval shell formation, nutrition, metabolism and apoptosis, respectively. We have cloned the three genes, investigated the temporal and spatial expression profile both at gene and protein level in trochophore (L1), D-veliger (L2), pediveliger (L3) and postlarvae (L4). The potential roles of these proteins were analyzed with specific inhibitors during larval development. Firstly, embryos were found developed into trochophore-like larvae with no shell if cultured at gastrula stage in artificial seawater without iron. Shell-like structures were formed only in the presence of iron. The larvae which had been transferred at L1 stage into ASW developed normal shell. This indicated that iron and iron associated protein are important for larval shell formation. The EST sequence which is homologous with ferritin, which is a principal iron metabolic protein, was selected from the M. meretrix cDNA library. The full-length of ferritin subunit cDNA was cloned by RACE. The results of real-time PCR revealed that the MmeFer mRNA expression changed before and after the larval shell formation.

本论文以文蛤幼虫为研究对象，分别对文蛤幼虫发育过程中贝壳形成相关的铁蛋白、营养及变态相关的组织蛋白酶B及变态过程中细胞凋亡相关的caspase三个基因进行了克隆，分析了基因及编码蛋白在担轮幼虫期(L1)、D形幼虫期(L2)、壳顶幼虫期(L3)和稚贝期(L4)的时空表达特征，解析了其可能的功能，并研究了相应酶类的特异性抑制剂作用对幼虫发育过程的影响，进行了目标蛋白的功能验证，详述如下：研究结果显示，在文蛤胚胎发育到原肠胚时放入不含铁离子的人工海水中培养，发育成无壳的畸形，随着人工海水中铁离子添加浓度的升高，幼虫长出壳状组织接近正常状态；而发育到L1期幼虫放入不含铁离子的人工海水中培养却可以发育出正常的壳，推测铁和铁代谢相关蛋白在幼虫贝壳初始形成有重要的作用。

Quantitative analysis of protein adsorption: The PS suspension containing lysozyme was subjected to ultracentrifuge (15 000 r/min) to collect clear aqueous solution. The lysozyme concentration in the clear solution was measured by spectrophotometry at 280 nm. The amount of protein adsorbed on PS surface was calculated based on the decrease in the protein concentration in the supernatant solution.

蛋白吸附定量分析：采用高速离心(15 000 r/min)法从含溶菌酶蛋白的聚苯乙烯悬浮液中分离出上清液，用紫外(280 nm)分光光度法测定经聚苯乙烯微球表面吸附后，液相蛋白质量浓度的下降值，由此推算蛋白在聚苯乙烯表面的吸附量。

Results the intakes of meats milk and fruits had increased 71.2g、9.0g and 22.7g respectively compared with 1992. high quality protein was increased and accounted for 46.9% of dietary protein.energy and protein intake was 2207.2kcals and 64.4gper reference man per day, accounted for 91.8% and 85.4% of rni respectively. fat provided 31.3% of energy.retinol, riboflavine wear lower,only accounted for 30.4% and 59.1% of rni respectively. the prevalence of undernourishment was 12.9%,overweight was 4.4% and obesity was 9.1%.

结果 与1992年结果比较，广西居民畜禽肉、奶类、水果等食品摄入量分别增加了71.2g、9.0g和22.7g，优质蛋白质来源达到46.9%；每标准人口摄入的能量、蛋白质分别为9226.2kj和64.1g，分别占推荐摄入量的91.8%和85.4%；而脂肪供给的能量占31.3%，视黄醇当量和核黄素摄入偏低，仅分别达到推荐标准的30.4%、59.1%，钙摄入仅达到适应标准的39.4%。

The RhoA oncogene , have been revealed in recent years, are closely related to various malignant tumours. RhoA, belonging to Rho family, is one member of the ras superfamily, whose encoding production, namely RhoA protein, pertaining to micromolecular mass GTP conjugated protein family, in GTP conjunctive active form and GDP conjunctive unreactive form. It exist in the latter form in normal cells and the switch of the two forms enables them to educe functions, paralleling"molecular switch", the diminutive G protein in activated state being able to activate the wink dromo-iter to work functions, which is in the resemblance of process during which the Ras genic mutation induces its continous activations.

RhoA是近年来新发现的与多种恶性肿瘤密切相关的癌基因，RhoA属于Rho亚家族，是ras基因超家族的一员，其编码产物RhoA蛋白属于小分子量GTP结合蛋白族，分为GTP结合的活性型和GDP结合的非活性型两类，在正常细胞内以GDP结合的非活性型存在，两种活性形式之间的转换使他们能够发挥一种类似"分子开关"的作用，活性态的小G蛋白能激活下游的信号传导通路，行使其功能。

Because the full length DFF45 recombinant protein was unstable in the solution, it means that either the DFF45 protein has uncompacted region in the structure or the protein has several domains.

由于细菌表达的全长DFF45重组蛋白表现出不稳定易降解的特点，说明该蛋白可能具有无固定结构的区域，或者含有多个结构域。

With the optimization of the cultivated conditions, the recombinant fusion protein staphylokinase-hirudin was over-expressed in E.coli BL21(DE3), and the final cell density reach 115g wet cell weight per liter, the target protein is about 1.1~1.2g/L accounting for more than 30% of the total protein. The culture process in 5L fermentor was performed on 40L fermentor successfully, and indicated that the process was easy scalable.

通过对培养条件的不断优化，重组葡激酶-水蛭素融合蛋白在大肠杆菌BL21(DE3)里得到了高效表达，菌体密度最终达到115g/L以上，可溶性重组融合蛋白占菌体总蛋白的30%以上，含量约为1.1~1.2g/L.5L发酵罐的发酵工艺参数在40L发酵罐中进行了放大培养，结果表明该工艺能有效的放大，可适用于工业生产。

In this research, the spider silk from the major ampullate gland of native Nephila pilipes was collected via the forced-silking apparatus at the reeling speed of 3 m/min. The obtained silk was dissolved in hexafluoro-isopropanol. The influence of pH and temperature over the secondary structure of the silk protein in solution state was examined via Circular Dichroism. The effect of silk concentration and electrical field strength on the structure conformation of silk protein during film casting process was investigated via FT-IR. During the drying process, the increase of silk concentration and the presence of electric field tend to form β-sheet and β-turn. Finally, the dried film of silk protein was treated with different temperatures and cation solutions to study the post-treatment effects.

本研究所采用之蜘蛛丝蛋白质系取自本土品种人面蜘蛛之大囊壶腺体，以3 m/min之卷丝速度强迫取丝(forced-silking)收集蜘蛛丝，再将纤维溶於六氟异丙醇溶剂中配制成溶液，以圆二色光谱仪分析在不同 pH 值与温度条件下丝蛋白质在溶液中之二级结构变化，再将蜘蛛丝溶液以不同浓度及电场强度下乾燥成薄膜后，以傅立叶红外线光谱仪检测分析丝蛋白质在固体状态下之二级结构，最后以不同温度加温、离子溶液浸泡等条件对乾燥所得之薄膜进行后处理，并分析其二级结构之变化。

Patients who are sensitive to protein,whose own body sensitive,due to one-time edibling a great deal of food that contains protein,such as egg,which is easy to suffer protein sensitive urticaria,diarrhea,bellyache,skin anthema and so on.

对蛋白质过敏一些本身体质过敏的病人，由于一次性摄人大量的含蛋白质食品，如蛋类等，易发生蛋白过敏性荨麻疹，出现腹泻、腹痛、皮肤出疹等现象。

AVI may be protein matrix and it possesses neither a membrane boundary nor an internal structure,its formation is the result of the anthocyanins transported into the vacuole bind with a protein matrix. In vacuole, AVI is irregular and jelly-like in shape. In AVIs, the attachment of anthocyanins to the matrix protein is likely to be via H-bonds to a sterically restricted site. AVI is suggested to act as vacuolar anthocyanin "trap", preferentially for anthocyanidin 3, 5-diglycosides or acylted anthocyanins. The emergence of AVI can enhance color intensity and results in the "blueness" of color in the vacuole.

花色苷液泡包涵体可能具备蛋白质基质，既无膜包裹又无内部结构，其形成是转运进液泡的花色苷与蛋白质基质结合的结果；液泡里的花色苷液泡包涵体形状不规则，象果冻；在花色苷液泡包涵体中，花色苷可能通过氢键连接于蛋白质基质的一个有限空间位点；花色苷液泡包涵体被认为是液泡中花色苷的&陷阱&，优先摄取花色素3，5-二糖苷或酰化的花色苷；花色苷液泡包涵体的存在可增加液泡色彩的强度并导致&蓝化&。

In the process of root cutting, the root accumulated a lot of soluble sugar and soluble protein, which provided material and energy for the cell differentiation. As root differentiating, soluable protein was continuously used up. During 10 to 20 days, the content of soluble sugar and soluble protein in the basipetal top and end of rootkept comparatively stable, then declined as adventitious buds and roots growing. 40 days later, they kept invariable, because the newly formed bud can photosynthesize and the newly formed root can absorb nutrient from outside. The dynamic balances of endogenous hormone regulate adventitious buds and roots of the root. During callus growing and adventitious bud differentiating.

上部根段在细胞分化前期积累了大量的可溶性糖，为后期的分化提供能源物质，同时积累了大量的可溶性蛋白质，为愈伤组织的产生提供能源，而后随着分化，可溶性蛋白质不断被消耗。10～20d根段上部及下部的可溶性糖、可溶性蛋白质含量保持在相对稳定的水平变化不大，后期随着不定芽及不定根的生长下降较快。40d后，新形成的芽已经能够进行光合作用，新形成的根也可从外界吸收营养物质，可溶性糖、可溶性蛋白质含量基本保持不变。

Nowadays, most of research are to build a transmutative Petri Nets through adding controlling place sets, controlling arc sets and controlling policy to the basic Petri Nets, while the Controlled Petri Nets could be used to argue many controlling theory problems conveniently and to induce many logically and physically supervisory and solve the Event Feedback Controlling Problems and State Feedback Controlling Problem in DEDS supervisory theory.

目前大多数的研究表现为在变形后的受控Petri网基础上，利用各种方法求得各种逻辑型、结构型控制器，解决DEDS监控理论中的事件反馈控制问题与状态反馈控制问题。

On one hand, there are discussions with the works council and union about extension of short time working up to the end of September.

一方面，有讨论，工程理事会和联盟关于延长工作时间短至9月底。