查询词典 protein crystal

As a result, Core gene was expressed in E.coli with yields of 20% of total proteins and it was a 31KD fusion protein on SDS-PAGE and Western-Blot, it shows that Core protein was highly expressed in E.coli;One fragment of 369bp could be seen on the gel of the Core gene PCR product. A band of 29KD could be seen on the Western-blot after induction of yeast containing pPICZaA-Core for 72 hours. This indicate that Core protein was secretively expressed in the Pichia pastoris with weak yields.

结果显示pBVIL1-Core的表达产物经SDS-PAGE分析出现一条约31KD的带，与预期融合蛋白的分子量相符，表达蛋白存在于包涵体中且表达量占菌体总蛋白的20%，Western-blot显示诱导后菌体在相应位置出现特异性杂交带，表明

The expressed protein accounted for more than 30 % of the total protein mass. Western blot confirmed that the fusion protein reacted to positive serum against sheep pox.

在IPTG 浓度为0.1 mM、温度为37 ℃、诱导6 h时表达量最大，约占菌体蛋白的30% Western blot 试验表明，融合蛋白可被羊痘阳性血清识别，这为研究其在新型疫苗和诊断的研究奠定了基础。

The gels were stained with silver, and the differential protein expressions were analyzed with Melanine 3 software. Results The protein spots of the two cell lines were 1285±51 and 1184±47 (SW480) respectively, containing 96±7 differential protein spots with 50±6 expressed or obviously increased only in LST cell line and 47±5 in SW480 cell line.

结果 LST和SW480细胞株双向电泳图谱蛋白质点数分别为1285±51和1184±47，共获得96±7的蛋白质差异点，其中50±6个点仅在LST细胞株中表达或表达明显增强，47±5个点仅在SW80细胞中表达或表达明显增强。

Purified protein samples were separated by 2-DE with 17 cm IPG strips, and the protein spots were detected by fast silver staining. Six protein spots were randomly excised from the silver stained gel for primary analysis by MALDI-TOF MS. Four proteins were identified, and they were 1-aminocyclopropane-1-carboxylate synthase 2, ribulose-1, 5-bisphosphate carboxylase/oxygenase large subunit, F11O4.2 and Rubisco small subunit.

根据预实验结果，选用17 cm IPG预制干胶条对纯化的蛋白样品进行2-DE实验，采用快速银染法进行染色，随机挖取银染胶上6个蛋白点进行MALDI-TOF MS分析和数据库检索，鉴定了3个蛋白，它们是：1-氨基环丙烷-1羧酸合酶2，核酮糖-1，5-二磷酸羧化酶/加氧酶大亚基和F11O4.2。

Different water bath and bath-time on the extraction plants in leaf protein Abstract :In the water bath for 70,80,90 ℃ for not using hot water bath time extracting heat No.2 Stylo leaf protein, and in which the crude protein content .

不同水浴温度和水浴时间对提取柱花草叶蛋白的影响摘要：在水浴温度为70、80、90、100℃条件下用不水浴热时间提取热研2号柱花草叶蛋白，并测定其中粗蛋白质的含量。

The results show :In the water bath temperature of 100 ° C, Bath time 5 minutes from the thermal No.2 Stylo leaf protein and LPC crude protein content is highest 柱花草:Stylo 叶蛋白:Leaf protein 水浴温度:Bath temperature 水浴时间:Bath time

结果表明：在水浴温度100℃、水浴时间5min时提取的热研2号柱花草叶蛋白和LPC粗蛋白质的含量最高。关键词：柱花草；叶蛋白；水浴温度；水浴时间

The thiolation of protein and the coupling reaction of thiol protein with liposomes have been investigated, it was found that the degree of substitution was different in spite of the same molar ratio of SPDP to protein.

研究了蛋白质的巯基化和巯基化的蛋白质与脂质体的偶联。

Shaw's research agenda that combines major advances in computational algorithms and architecture to provide the requisite thousand-fold increase in processing speed to simulate protein folding and protein-protein interactions in a biologically meaningful timeframe.

这是生物学上崭新的一页。萧研究的课题是综合计算机算法和信息技术学的主要成果，以提供处理蛋白质折叠，加速模拟生物学"蛋白质折叠和蛋白质-蛋白质交互作用"。

Results Same as hyaline cartilage, the calcified cartilage was stained light flavo-green and weak double refraction by the Picrosirius-polarization method. In the immunohistochemistry analysis of collagen Ⅱ protein, the calcified cartilage was stained strong green fluorescence and the collagen protein parallelled in joint surface near the tidemark. The content of collagen Ⅱ protein was (20.16%±0.96%) of tissue dry weight by amino acid analysis, and that of the hydroxyapatite was (65.09%±2.31%) of the inorganic composition in calcified cartilage.

结果 天狼猩红染色见钙化软骨层与透明软骨层一样黄绿色淡染、弱双折光性；Ⅱ型胶原蛋白免疫组织化学分析，钙化软骨层呈强绿色荧光，潮线附近的胶原蛋白呈板层平行分布；氨基酸定量分析钙化软骨层Ⅱ型胶原蛋白含量占组织干质量的（20.16%〖KG-*4〗±0.96%）；X-射线衍射定量分析钙化软骨层中的羟基磷灰石含量占无机成分的（65.09%±2.31%）。

For the development of protein drugs and the research of biochemistry, it is significant to probe the interaction between chitosan and protein, and to observe changes of adsorption when both of them contact at interface. Total internal reflection fluorescence spectroscopy is an effective technique to investigate protein adsorption at interface.

壳聚糖对蛋白质有良好的亲和能力，研究蛋白质与壳聚糖在界面上的相互作用、理解蛋白质与壳聚糖分子在界面接触时的吸附过程变化对蛋白药物的开发和生物化学研究具有非常重要的意义。

Nowadays, most of research are to build a transmutative Petri Nets through adding controlling place sets, controlling arc sets and controlling policy to the basic Petri Nets, while the Controlled Petri Nets could be used to argue many controlling theory problems conveniently and to induce many logically and physically supervisory and solve the Event Feedback Controlling Problems and State Feedback Controlling Problem in DEDS supervisory theory.

目前大多数的研究表现为在变形后的受控Petri网基础上，利用各种方法求得各种逻辑型、结构型控制器，解决DEDS监控理论中的事件反馈控制问题与状态反馈控制问题。

On one hand, there are discussions with the works council and union about extension of short time working up to the end of September.

一方面，有讨论，工程理事会和联盟关于延长工作时间短至9月底。