查询词典 pronucleus

It is concluded that it is MAPK, not p90rsk,that is related with porcine oocyte MII block and pronucleus formation.

在MAPK维持猪卵母细胞MII阻滞当中是否存在其它底物有待今后进一步研究确证。

After lOmin delay only 81.2% pronucleus developed to blastula, significantly lower than control (93.2%).

滞留20min不引起原核胚、2-cell胚胎和桑椹胚以及囊胚的死亡。

Methods HBV DNA was extracted from the serum of patient with hepatitis B. The X gene was amplified by PCR using the primers with EcoRI and HindⅢ digestion sites, and then cloned into pronucleus expression vector pMAL-C2X, which was detected by EcoRI and HindⅢ digestion and sequence.

从乙肝病人血清中提取HBV DNA；以带有EcoRI和HindⅢ酶切位点的引物，用PCR方法扩增X基因；而后定向克隆到原核表达载体pMAL-C2X上，经酶切鉴定和序列分析；以IPTG诱导X融合蛋白的表达。

Theproduction efficieny of transgenic animal by the traditional method of pronucleus microinjection isvery low (The effciency of transgene is about 3～5％.), which seriously restricted the developmentof transgenic animal research.

以传统的原核显微注射法生产转基因动物的效率非常低下(成功率仅为3～5％)，严重地制约了转基因动物研究进展。

Oocytes injected with frozen-thawed sperm were activated by various methods, and the effect on the pronucleus formation and blastocyst formation rate were examined.

方法］冷冻精子解冻洗涤后直接应用于ICSI，ICSI后采用不同激活方法激活卵母细胞，检测其原核形成情况。

In fact, 9 years Qian Xinhua introduced exterior 4 tall canals, altogether of company of eye pronucleus center 6 tall canals, among them two familial personages that are Chen Fashu.

事实上，9年前新华都就引入了外部四个高管，现在核心子公司一共六个高管，其中两个是陈发树的家族人士。

We also successfully amplified Sry from pronucleus stage embryos, which prove that embryo can be sexed by Sry PCR method using one cell instead of the minimal detectable cell amount of 4 cells proved by others.

同时由于从原核时期扩增到了Sry，本文认为胚胎的性别鉴定取单个细胞即可。

Mitochondrion was the largest Ca〓 store. There was no or less Ca〓 in germinal vesicle, spindle and pronucleus.

生发泡破裂时其周围膜结合Ca〓分布丰富，可能是由于线粒体于该阶段在该处集中分布所致；（11）。

Then the linear vector was microinjected into the pronucleus of the KM×B6D2F1 zygotes to produce the transgenic mice. Thirty-four adult mice were produced from 286 of injected embryos.

用显微注射方法，将线性化的载体DNA注入KM ×B6D2F1小鼠受精卵的的原核内部，然后将胚胎移植到输卵管内，13只受体怀孕产仔。

Methods: the hbv transgenic mice were generated by microinjection of 1.3 copies hbv genome into the pronucleus of fvb/n zygotes. the pcr assay,elisa,rt-pcr and immunohistochemical methods were used to detect the hbv integration,replication and expression in the transgenic mice.

采用受精卵显微注射法，制备hbv转基因小鼠，用pcr，elisa，荧光定量pcr和免疫组化的方法研究hbv基因在转基因小鼠体内的整合、复制和表达情况。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。