查询词典 pronuclei

To obtain the fertilized eggs with clear male pronuclei is the key of success .

显微注射法是制备转基因动物的首选方法，而原核清晰受精卵的获得是影响显微注射成败的关键。

These results indicate that,(1) appropriate concentration of CHX can enhance the activation efficiency of oocytes;(2) CB can increase the two pronuclei formation rate of oocytes by inhibiting the extrusion of second polar body.

这些结果说明：(1)适当浓度的CHX能提高卵母细胞激活效果；(2)CB能抑制卵母细胞激活后第二极体排出，从而提高原核的形成率。

These results indicate that CHX at an appropriate concentration can improve the parthenogenetic development of buffalo oocytes, CB at an appropriate concentration may inhibit the extrusion of the first polar body and increase the proportion of activated oocytes with two pronuclei.

结果表明，适当浓度的CHX能提高卵母细胞激活效果，适当浓度的CB可抑制卵母细胞第二极体的排出，提高孤雌激活后双原核的比例。

Addition of 3μg/ml CB to CM containing 0.625μg/ml CHX increased the proportion of oocytes with two pronuclei (38. 9% vs 15. 1%, P＜0.05) and developed into morulae/blastocysts (40.7% vs 26. 8%, P＜0.05), but did not affect the percentage of oocytes cleaved and total pronucleus rate.

如果在含有0.625μg/ml CHX中添加3μg/ml CB，可明显提高双原核的形成率（38.9％vs 15.1％，P＜0.05）和桑椹胚/囊胚发育率（40.7％vs 26.8％，P＜0.05），但对其激活分裂率和总原核形成率无明显影响（P＞0.05）。

When the porcine-porcine and porcine-mouse nuclear transfer embryos were activated,inthe former only one pronucleus was observed in most cases,but in the later two pronuclei werefound mostly.

猪-猪同种核移植胚胎和猪-小鼠异种移核胚胎激活后，异种重组胚多形成二原核(2PN)，而同种重组胚则主要形成一原核。

Icrofilaments were mainly distributed in the cortex area which chromosomes located at mataphase of second meiosisand concentrated around pronuclei after pronuclear formation.

II 期卵母细胞微丝主要集中在染色体所在的皮层区域，原核形成后微丝绕核重新聚集。

No embryos developed to term after transfer of the pronuclei stage embryos to oviducts of pseudopregnant mice.

将体外受精得到的重组卵来源的双原核胚胎进行输卵管移植，没有胚胎发育到期。

DNA was microinjected into male pronuclei of fertilized mouse eggs,the injected mouse eggs were then transplanted into the oviduct of pseudopregnant female mice.

摘 要：目的建立人纤溶酶原激活物抑制物1(PAI-1)转基因小鼠。方法利用构建的高效真核表达载体pCAGGS-PAI-1，制备注射用DNA。

These zygotes were divided into four groups according to the different zygote pronuclei orientation and the developmental potential.

根据早期卵裂时间及原核构型不同进行筛选而移植的129个体外受精、卵胞浆内单精子注射周期，共1 032个正常受精卵为资料。

We spliced the pronuclei vector and microinject the remain DNA sequence to pronuclei of the mouse zygote.We got 29 mice of G0 generation ,and 8 of the 29 mice are positive result which checked by method of PCR.

构建的乳腺特异性表达载体去除原核部分(αS1-casein基因调控序列+人胰岛素原基因)，显微注射小鼠受精卵雄原核，获得29只G_0代小鼠，PCR检测出其中8只为转基因刚性小鼠。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。