查询词典 pronuclear

Methods Regulation region of bovine β-lactoglobulin geneand full-length genomic DNA encoding hEPO were used to construct eukaryotic expression vector,and the transgenic goats were produced by pronuclear microinjection.

方法采用牛β-乳球蛋白基因调控元件和hEPO全长编码序列基因组DNA构建真核表达载体，应用受精卵原核注射的方法制备hEPO转基因山羊。

So far, the mammalian pronuclear transplantation is mainly studied on mice.

迄今为止，哺乳动物的原核置换研究主要在小鼠上进行。

A total of 38 males and 24 femals irradiated at the pronuclear stage survived and were available for testing.

在原核期受照中共有38只雄性和24只雌性存活下来，并可供试验。

So, firstly we studied pronuclear embryo of rabbit by sequence culture that is a more developed in vitro culture system.

因此，我们首先对兔原核期胚胎采用一种较先进的体外培养系统——序贯培养进行了研究。

The technique of pronuclear microinjcetion is the most reliable and popular method for transgenic livestock production to date.

到目前为止，原核注射是最可靠，也是使用最广泛的动物转基因方法。

The relationship between cytoplasm and nucleus is the basic problem of biology, and nuclear transfer and pronuclear transplantation are the important methods to research their relations.

细胞核与细胞质的关系是生物学的基本问题，核移植和原核置换是研究核质关系的重要方法。

Development ability of sheep embryos after pronuclear injected withDNA was studied.

研究了显微注射对绵羊体外受精胚胎发育的影响。

The main objective of the dissertation was to improve and modify the experimental procedures of mouse nuclear transfer and pronuclear transplantation, respectively, and try to establish new possible methods for mouse cloning and pronuclear transplantation.

本论文的主要目的是改进和修改小鼠核移植和原核置换的实验程序，试图建立新的小鼠核移植和原核置换的可行方法。

Penetration of sperm, pronuclear and blastocyst formation were observed.

结果 经改造的培养液HTFplus和IVF-30，体外受精率分别从13%、15%提升至70%、67%。

In conclusions,(1) The pronuclear formation was asynchronous after ICSI in buffalo, the female pronuclear formed 3 hours earlier than male pronuclei;(2) After ICSI and activation, Culture of oocytes in 1.9mmol/L 6-DMAP for 3 hours can improve their subsequent embryonic development;(3) Treatment of sperm with 5mmol/L DTT can promote sperm decondensation;(4) Dead sperms can be used for ICSI in buffaloes;(5) Treatment of sperm with GSH can improve the efficiency of ICSI in buffaloes.

以上结果表明：(1)水牛精子胞质内显微受精的雌、雄原核发育不同步，雌原核比雄原核早3h形成；(2)水牛卵母细胞ICSI和激活后用1.9mmol／L 6-DMAP培养处理3h能提高其胚胎发育率；(3)DTT预处理水牛精子能提高其ICSI后的精子解聚率；(4)死精子能用于水牛卵母细胞的ICSI；(5)GSH预处理水牛精子有助于提高其ICSI后的囊胚发育率。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失