查询词典 promoter

And then, the pBI101.2 wirelike vector and the promoter fragment of 886bp were acquired. Becase the promoter was modified by the HindⅢand BamH I, the acquired target genes fragment, digested by the restriction endonuclease, was matched to the wirelike vector.

由于在启动子两端有人工设计合成的HindⅢ和BamHⅠ酶切位点，经上述双酶切消化后，回收目的基因片段与线状载体的粘性接头恰好匹配。

There are two elements, a CSF-box and a octamer within G-CSF promoter are essential for its promoter activity. The CSF-box is a potential NF-κB binding site, and the octamer was bound by Oct-2.

当以U0126抑制MEK1/2活性而阻断LPS所活化的MEK/ERK讯息传递路径时，发现LPS所引发的G-CSF表现量显著地下降下来。

Taken together, our data showed that both the CSF-box and the octamer are important for the LPS-induced G-CSF promoter activity, moreover, the activation of MEK/ERK signaling pathway is indispensible for LPS-induced G-CSF expression in RAW264.7 macrophage.. It seems that LPS-induced activation of MEK/ERK pathway does not directly regulates NF-κB transactivity, but is essential for LPS-induced Oct-2 binding to the octmaer within G-CSF promoter and transcription activation of the G-CSF expression.

透过这些结果，我们认为MEK/ERK pathway的确参与LPS活化G-CSF表现的过程，虽然MEK/ERK pathway都影响了CSF-box以及octamer对G-CSF启动子所贡献的活性，但似乎MEK/ERK pathway的下游并非直接影响NF-κB调控基因转录的活性，而主要是影响了Oct-2对G-CSF启动子的调控。

The results showed that OsAgpS1a transcripts and the reporter gene GUS driven by the OsAgpS1a promoter had a similar expression pattern that abundantly expressed in endosperms but very low in leaves, whereas OsAgpS1b transcripts and the reporter gene GUS driven by the OsAgpS1b promoter own other similar expression pattern that expressed at a low level in leaves, roots and early development endosperms of rice.

结果表明，两个启动子与其下游转录本的表达模式完全一致，即OsAgpS1a转录本和OsAgpS1a上游启动子控制的GUS基因主要在胚乳中高水平表达，在叶片中有很低水平的表达；而OsAgpS1b转录本和OsAgpS1b上游启动子控制的GUS基因主要在叶片和胚乳发育早期低水平表达。

The sequence （GenBank accession DQ77520） contained a novel promoter region with TATA box,CAAT box and CA2 box-like elements in the identical positions common to the eucaryote promoter sequence regions in comparison with the sequences in the GenBank.

对该产物测序并提交GenBank数据库（GenBank accession DQ677520），经序列比对分析，发现该序列具有真核启动子序列的基本结构特征，含有TATA盒、CAAT盒、GC盒等元件。

A new expression vector with the promoter of the medaka β-Actin promoter and green-fluorescent protein gene was constructed based on Nde I site of pBluescript SK+.

从载体pBluescript SK+的Nde I位点处插入外源基因片段，构建了含有青鳉β-肌动蛋白启动子和绿色荧光蛋白标记基因的新表达载体。

Among seven different constructions we examined, a DNA fragment, named pCMV-MB-EGFPITR, containing EGFP reporter gene driven by human cytomegalovirus promoter and medaka β-actin promoter, produced the most intense GFP expression.

在我们试验的七个不同构筑质体中，被命名为pCMV-MB-EGFPITR的DNA片段，含有被人类巨细胞病毒启动子和稻田鱼β型肌动蛋白启动子所驱动的增强型绿萤光蛋白报导基因，能产生最强的绿萤光表现。

In order to confirm the influence of HCV persistent infection on IRF and type I interferon, HCV replicon cell (Huh7 cell line harboring subgenomic HCV replicons) was used.

结果发现，在NDV攻击下，Replicon细胞的IRF-7 promoter活性、内源性IRF-7和IFN-α表达水平均较Huh7细胞低(IRF-7 promoter活性上调倍数：2.54 vs 4.20，P＜0.001；内源性IRF-7mRNA表达水平：0.137 vs 1.671，P＜0.001；内源性IFN-αmRNA表达水平：0.246 vs 2.207，P＜0.001)。

In order to elucidate the regulation mechanism of RU5 region to BFV gene expression, BFV3026 provirus DNA was used to construct the plasmids containing different deletion of R region, which were cotransfected with luc report gene locatied behind the IP promoter to BL12 cells, and luciferase activities was assayed, confirming that U5 region could repress the initiation function of LTR as well as IP. The BFV structure genes with different deletion of R region were cloned closely behind to heterogenous CMV promoter, then transfected to 293T cells, RT activity was performed, testifying the R region was required for BFV pol gene expression, and also the function domain was identified within the 100n.t. sequence at the 5′end.

以牛泡沫病毒(Bovine foamy virus， BFV)中国株BFV3026原病毒DNA为材料，构建R区系列缺失质粒，通过对其转染细胞中RT水平及对缺失质粒与luc报告质粒共转染细胞中萤火虫荧光素酶活性的测定，确立U5区对于BFV3026两类启动子LTR和IP均具有负调控作用；同时将带有不同R区的BFV3026结构基因片段克隆于异源启动子CMV之下，通过对其转染细胞293T中RT酶活性的测定，确立R区对于病毒结构基因pol的表达具有一定的调节作用，并将其功能区域初步界定在R区5′端100bp内。

Consequently,in this study we introduced genes that encode the xylose isomerase and xylulokinase precisely under the control of a strong,constitutive glyceraldehydes-3-phosphate promoter by PCR-mediated overlap extension,which is in charge of xylose assimilation;and also we introduced genes that encode the transaldolase and transketolase precisely under the control of enolase promoter,which is in charge of xylose utilization,then transformed plasmid with the two operons into Z.

因此，本文通过代谢工程手段，将负责木糖代谢转化的木糖异构酶基因和木酮糖激酶基因置于强组成型启动子3-P-甘油醛下；同时将负责木糖转化利用的转醛醇酶和转酮醇酶基因置于强组成型启动子烯醇酶下，通过电转的方式将上述四个基因转入到Zymomonasmobilis CP4中，从而在运动发酵单胞菌中构建起一套完整的木糖代谢途径。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。