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The composite electroforming process of preparing nanometer alumina particle reinforced copper base composite material has phosphor copper plate as anode material, stainless steel sheet as cathode deposition body, nanometer level alumina particle as reinforcing particle, and fluorocarbide surfactant or formamide as co-deposition promoter.

一种纳米氧化铝颗粒增强铜基复合材料的复合电铸制备方法，用金属磷铜板作阳极材料，不锈钢片作为阴极沉积体，增强颗粒为纳米级氧化铝颗粒，采用氟碳阳离子表面活性剂或甲酰胺为共沉积促进剂。

By a two-promoter vector, we successfully coexpressed DsbA-DsbC fusion protein with hNGF in procaryote cytoplasm and obtained the soluble hNGF protein, but the DsbA or DsbC alone do not show this function.

通过本课题的研究，我们根据DsbA和DsbA~蛋白的性质特点获得了一种原核通用融合表达载体，该载体在表达真核来源的活性蛋白或细胞因子具有促可溶和保持目的蛋白天然活性的功能。

These results suggested that the function of each function domain in HIV 1 LTR exhibited distinct characteristics on gag gene expression in vaccinia virus:(1) The function of intact LTR on gag gene expression varied with the upstream poxvirus promoter;(2) NR sequence neither decreased nor increased the Gag protein exprssion level;(3) Enhancer sequence might not be recognized by recombinant vaccinia virus expression system;(4) TAR sequence enhanced Gag protein expression effectively;(5) U5 region and its downstream non translated sequence had little effect on

免疫印迹和免疫酶试验检测均表明，6株重组病毒的Gag蛋白表达量因LTR不同而有明显差异，表明HIV1的LTR及其下游基因置于痘病毒启动子控制下，在痘苗病毒中表达时有下述特点：（1）不同的痘苗病毒启动子与全长LTR相互作用，对gag基因表达有显著不同的调控效果；（2）NR序列对Gag蛋白表达没有明显影响；（3）EN序列不能被重组痘苗病毒表达系统识别；（4）TAR序列可提高Gag蛋白的表达量；（5）U5区及下游非翻译序列不影响Gag蛋白的表达。

After analyzing the Sp1 DNA sequence within 5'-UTR and promoter region, we found one typical IRES-conserved sequence localizing within 5'-UTR of Sp1 genomic sequence. The reporter assay and polysome distribution assay revealed that Sp1 could be accumulated strongly and rapidly under hypoxia/ischemia neuron cells.

在Polysome distribution assay的结果显示，Sp1在缺糖缺氧之后转译效率有提高的情形，接著分析Sp1 5'-UTR，发现可能存在一个典型的IRES结构，利用bicistronic assay也证实了Sp1的堆积是透过IRES所导致。

Three plasmids named pBSGt pBSGd and pBSGk were constructed respectively with two plasmids named pEGH and pBS305. The plasmid pEGH contained gfp gene and pBS305 is an E. coli -Rhodococcus shuttle vector. These constructed plasmids can be used to detect the efficiency of promoter.

以带有报告基因gfp的质粒pEGH和大肠杆菌-红球菌穿梭载体pBS305为出发质粒，构建了三个带有不同启动子的表达质粒pBSGt、pBSGd和pBSGk，电击转化红平红球菌LY822。

Methods Human EGFR cDNA fragment was subcloned in the oppos ite orientation to the cytomegaloviral promoter and inserted into a E1/E3-delet e d type 5 adenoviral vector to obtain AdE5 construct which expresses EGFR antisen se RNA.

人EGFR的cDNA片段被反向亚克隆入E1/E3缺失的 5型腺病毒载体中而得到AdE5 ，EGFR反义RNA的表达由CMV启动子控制。

Different NodD operons are highly conserved in their promoter DNA sequences.

不同的NodD操纵元具有高度保守的启动子序列。

Transcribed sequence just downstream of the trp operon promoter that is central to the attenuation mechanism.

紧接在trp操纵子的启动子之后转录出来的序列，对衰减作用机理很重要。

Attenuator Transcribed sequence just downstream of the trp operon promoter that is central to the attenuation mechanism.

衰减子 紧接在 trp 操纵子的启动子之后转录出来的序列，对衰减作用机理很重要。

Oncolysis and suppression of tumor growth by a GFPexpressing oncolytic adenovirus controlled by an hTERT and CMV hybrid promoter［J］.

人端粒酶催化亚单位启动子驱动EGFP真核表达载体的构建及在肺癌细胞的表达［J］。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。