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Fourthly, the expression system containing P〓 promoter and phoA signal peptide was used to express superactive glucagon,-glucagon (SA-glucagon).

四 此外，采用上述体系表达了胰高血糖素的高效激动剂—超活性胰高血糖素。

By PCR amplification and southern blot analysis, the extrogenous gene with specific promoter was confirmed to integrate into the supersweet maize genome DNA with single-copy, and transformation frequency could reach as high as 9.09%.

经PCR和Southernblot分析，证明抗虫基因已经整合在超甜玉米基因组中，并且为单拷贝。T1代植株PCR检测结果表明，Bt基因能够在转基因后代中稳定遗传，基因分离符合1∶1的孟德尔遗传分离规律。

The aim of this study was to evaluate the possibility of dietary supplementation of probiotics, prebiotics and symbiotics in broiler to replace the antibiotic as growth promoter, by compare the growth performance and immune response.

中文摘要本研究之目的为评估肉鸡饲粮中添加益生菌、益生剂与合生剂对肉鸡生长性状与免疫反应之影响，并探讨其取代抗生素作为生长促进剂的可行性。

ACBF is a transcription factor that combined with the AC-rich region of the promoter region. It has been cloned at Arabidopsis thaliana, Nicotiana tabacum and so on.

ACBF编码了结合在苯丙氨酸解氨酶启动子AC富集区上的一个转录因子，ACBF基因已经在拟南芥、烟草等植物中得到了克隆。

Tabacum with a human lactoferrin cDNA under the control of the 35S promoter from cauliflower mosaic virus, using A.

人类的乳铁蛋白之 cDNA，在花椰菜镶嵌病毒的35S启动子控制下，转型於菸草上之后，利用 A。

Methods The hTERT promoter as E1A and E1Bp gene were amplified by PCR; the GFP gene and GM-CSF were also amplified by PCR, and the products were subcloned into Teasy plasmid in certain order to generate pSh-GFP-SV55K plasmid.

采用PCR的方法克隆腺病毒E1区基因(E1A， E1B55K)、EIB的启动子(E1Bp)以及人端粒酶逆转录酶亚基启动子，以及2个目的基因：人GM-CSF和GFP(Green Fluorescence Protein， GFP)。

Three expression vectors containing mutant gfp which were truncated from 3′ termine were constructed in the present paper.The mutation gfp with different length under the control by T 7 promoter were expressed in E. coli BL21 cells.

本文将突变型 gfp3′末端不同程度地截短，构建成表达载体，在大肠杆菌 BL 2 1中表达了不同长度的绿色荧光蛋白，当其生色基因中 65位 Ser点突变成 Thr，则 GFP的激发波由 396nm增至 4 70 nm，不用 UV激发，仅在自然光源下就可看到强烈的绿色荧光，发光强度增加 6倍，若从 3′端截短 12个氨基酸密码，对发光无影响，但截短 75个氨基酸密码，则激发波长降到 360 nm，发光强度减少 12倍。

In this study,According to known sequence of Arabidopsis thalian cold-induced promoter rd29A,we cloned a 953bp distinctive fragement.

由于本研究中采用的是反义基因技术，个别碱基的突变并不影响该基因在反义RNA中的功能。

To elucidate the function of OsBIRHl in disease resistance response, we cloned OsBIRHl into a plant transformation binary vectoe pCAMBIA 99-1 under control of the CaMV 35S promoter and transformed Arabidopsis thalian via flora dipping.

为了研究水稻OsBIRH1在抗病反应中的可能作用，我们构建了OsBIRH1的转基因双元载体，并用浸花法转化拟南芥。

Then the vectors were transformed to Torenia fournieri to validate the function of the promoter.

该启动子的克隆对于研究蓝猪耳胚珠TfPLC1基因的表达调控及功能具有重要意义。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。