查询词典 proliferation

Keywords otocyst cells; stem cells; cell proliferation; differentiation; fetal rats

听囊细胞；干细胞；细胞增殖；细胞分化；胎鼠

The present study is to culture otocyst cells in fetal, cochlear sensory epithelial cells of neonatal and mature respectively in vitro with refinement of culture media and techniques. Immunocytochemistry and Bromodeoxyuridine labeling are used to detect properties and mitotic status of cultured cells. With the observations about proliferation and differentiation in cultured cells, we want to find the origin of regenerative cells and to understand how these progenitors proliferate and differentiate.

本研究分别对胎鼠听囊、新生鼠听觉上皮和成年鼠听觉上皮用自然培养方法进行体外培养，并应用免疫细胞化学方法，对培养细胞进行染色标记和观察，系统了解哺乳动物听觉细胞发生、发育过程，以及这些细胞在体外的增殖和分化过程，旨在了解哺乳动物听觉细胞听觉上皮感觉细胞的来源，发现细胞生长的前体细胞。

METHODS: Otocyst epithelia surgically isolated from fetal rats were cultured and passaged. Proliferation and differentiation of cultured cells were observed using BrdU labeling to find the progenitors. Immunocytochemistry (Factin, cytokeratin and calretinin) was used to detect cell properties of cultured cells.

取孕鼠胚胎听囊细胞进行体外培养，用免疫细胞化学方法，分别对培养的细胞进行细胞角蛋白、F肌动蛋白和calretinin染色观察细胞特征；BrdU染色观察细胞增殖。

METHODS摘要: Otocyst epithelia surgically isolated from fetal rats were cultured and passaged. Proliferation and differentiation of cultured cells were observed using BrdU labeling to find the progenitors. Immunocytochemistry (Factin, cytokeratin and calretinin) was used to detect cell properties of cultured cells.

方法摘要：取孕鼠胚胎听囊细胞进行体外培养，用免疫细胞化学方法，分别对培养的细胞进行细胞角蛋白、F肌动蛋白和calretinin染色观察细胞特征；BrdU染色观察细胞增殖。

In different brain regions over-excited, and follow a complex neural network approach to the proliferation and spread, leading to a wide range of clinical epilepsy and epilepsy syndrome, contains a variety of causes, clinical symptoms, EEG characteristics of as well as treatment and prognosis of reactions.

不同大脑区域的过度异常兴奋以及循着复杂的神经网络途径进行扩散和传播，导致了临床上多种多样的癫癎发作，而癫癎综合征则包含了不同的病因、临床症状、脑电特征以及治疗和预后反应等。

Overhigh concentration of insulin (10 nmol/L、100 nmol/L) has none or only minor promotive effect in EPC proliferation and NO secretion.

过高浓度的胰岛素(10 nmol/L、100 nmol/L)对EPC增殖、衰老、黏附和NO分泌的促进作用减弱。

Objective To investigate the effects of oxidative stress on the proliferation of rat hepatic stellate cells.

目的 探讨H2S对大鼠肝星形细胞增殖和氧应激的影响。

The adenovirus plasmid was identified by PacI digestion and transfected into 293A cells to package a recombinant adenovirus which expressed the Fhit protein. Furthermore, the adenovirus rAd-Fhit was infected into colon cancer cells,and the expression of the ectogenic protein was detected by Western blotting. Finally, the proliferation of colon cancer cells was observed in adenovirus-infected cells by the MTT assay.

经PacI酶切鉴定正确后，将重组腺病毒质粒转染293A细胞获得表达Fhit蛋白的重组腺病毒rAd-Fhit，将获得的重组腺病毒感染结肠癌细胞，采用蛋白印迹法检测外源Fhit蛋白的表达，并进一步观察其对细胞增殖能力的影响。

Objective: To study the effect of retinoic acid and basic fibroblast growth factor on neural stem cells＇ proliferation and differentiation from new born rats＇ pallium.

目的：研究视黄酸、硷性成纤维细胞生长因子对体外培养的神经干细胞分裂增殖和分化的作用。

NSCs were isolated from the brains of new born Sprague-Dawley rats＇ pallium, and the features of cells were characterized by immunofluorescence staining. The effects of different culture medium on survival and proliferation of cells were determined by MTT assay. The effects of bFGF and RA on differentiation of NSCs were observed.

分离培养新生SD大鼠大脑皮层NSCs，采用不同组合配方的培养液培养细胞，利用MTF法检测其对NSCs存活和增殖的影响；利用免疫荧光鉴定和分化细胞的分类计数法，判定bFGF、RA等对NSCs分化的影响。

Listen,point and check your answers.

听，指出并且检查你的答案。

Warming needle is one of effective treatment methods for knee arthralgia aggravated by cold,and it is simple,safety,so it should be developed in clinical acupuncture and moxibustion extensively.

但以本院科针灸门诊在2005年1月—2006年6月期间共收治膝痛患者100余例，经过临床的诊断后，其中施以温针治疗的48例，疗效显著，报道如下。1临床资料本组病例48