查询词典 proliferation

Get high purity DCs by Cultured plastic-adherent monocytes isolated from healthy human peripheral blood with GM-CSF and IL-4 for 7 days. To observe the morphology of DCs by inverted phase contrast microscope ,electron microscope and laser confocal microscope. Analyse phenotype of DCs with flow cytometry. Investigate the endocytosis ability of DCs as a group by Horseradish peroxidase endocytosis assay. To appraise allogeneic mixed lymphocytes reaction of DCs by MTT reduction assay. Analyse the levels of IL-12 and TNF in liquids of cultured medium by ELISA and MTT reduction assay respectively. Soluble antigens of HCCs was obtained by 3 freeze-and-thaw cycles. Biological characteristics of HC soluble antigens pulsed DCs were monitored by flow cytometry. According to MTT reduction assay estimated the cell proliferation of self lymphocytes activated by HC antigens pulsed DCs. Get high purity BCG HSP 70 protein by SDS-PAGE electrophoresis and determined its biological activity with ELISA. Analyse phenotype of antigen pulsed DCs primed by BCG HSP70 with flow cytometry. By MTT reduction assay estimated the cell proliferation of self lymphocytes and the MLR of DC based vaccine. Analyse expression of HLA-DR molecule on surface of HCC lines. The IFN-γ mRNA in lymphocytes after actived by DC vaccine and the Fas-L expression on DC and DC vaccine primed lymphocytes were detected by in situ hybridization and flow cytometry respectively. Specific cytotoxity lysis of T lymphocytes and nonspecific inhibition of liquids in culture medium against HCC lines were also tested. Detect expression of hAFP on four HCC lines with Cell-ELISA. Induce apoptosis of HCCs with actinomycin-D. Interaction of DCs and apoptotic cells was observed under transmission electron microscope. Growth inhibition test of DC against HCC lines was also performed. Establish the nude mouse model bearing human HC xenografts and indentify the characteristic of tumour by histochemistry and immunohistochemistry techniques. Prevent and treat transplanted human HC on nude mouse with Freezing and anabiotic HC specific lymphocytes.

用GM-CSF和IL-4从健康人外周血诱导DC；分别用倒置相差显微镜、电子显微镜及激光共聚焦显微镜观察DC形态；流式细胞术检测DC表型；HRP吞噬实验测定DC的群体内吞能力；MTT法检测同种异体混合淋巴细胞反应；ELISA法和MTT法分别测定DC培养上清液中IL-12和TNF水平；冻融法制备肝癌细胞可溶性抗原；流式细胞术检测负载肝癌可溶性抗原后DC的生物学特性；MTT法检测DC负载肝癌抗原后对自身淋巴细胞增殖的影响；SDS-PAGE制备电泳纯化BCG HSP70并鉴定纯度，ELISA测定活性；流式细胞术检测负载抗原DC经BCGHSP 70活化后的表型；MTT法检测肝癌DC疫苗对自身淋巴细胞增殖的影响和混合淋巴细胞反应；流式细胞术检测肝癌细胞表面HLA-DR表达；MTT法检测肝癌DC疫苗对自身淋巴细胞的活化；原位杂交法检测肝癌DC疫苗活化后的淋巴细胞IFN-γmRNA表达；流式细胞术检测DC和肝癌DC疫苗活化后淋巴细胞表面Fas-L；MTT法分别检测肝癌DC疫苗活化的淋巴细胞和其培养上清对肝癌细胞的特异性杀伤和非特异性抑制作用；Cell-ELISA检测人肝癌细胞hAFP表达；MTT法检测负载AFP表位肽和凋亡肝癌细胞DC对自身淋巴细胞增殖的影响；ELISA法和MTT法分别测定活化后淋巴细胞培养上清中TNF和IL-12水平；肝癌细胞凋亡的诱导和检测；DC吞噬凋亡肝癌细胞后的电子显微镜观察；DC对肝癌细胞的生长抑制试验；人肝癌裸鼠皮下移植瘤动物模型的建立及其组织学和免疫组织化学鉴定；DC及肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤；冻存和复苏后的肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤。

EGF has the auxoaction on proliferation of the cultured fibroblasts in vitro. Collagen scaffold has some regulatory effects on proliferation rate of the cultured fibroblasts in vitro.

EGF对FB的增殖具有促进作用，而胶原网架对FB的增殖速率具有一定的调节作用。

AIM: To study the effect of volatile oils from Boswellia carterii Birdwood on the proliferation and apoptosis in human liver cancer SMMC-7721 cells; METHODS: Effects of proliferation were detected on SMMC-7721 by volatile oils from B.

目的：探讨乳香挥发油体外抑制人肝癌SMMC-7721细胞株增殖及诱导凋亡的作用。

Methods:The proliferation inhibition of cantharides injections on LoVo cells were detected by tetrazolium.Annexin V-FITC were applied to study cell apoptosis of LoVo cells.FCM were applied to detect the expressions of the Fas and Bcl-2 protein.Results:Cantharides iniections could inhibit the proliferation of LoVo cells and could couse cell apoptosis.

四甲基偶氮唑盐测定药物对LoVo细胞抑制作用与时间和剂量的关系，Annexin V-FITC方法对细胞凋亡进行检测，流式细胞术检测细胞Fas、Bcl-2蛋白表达水平结果：MTT实验表明复方斑蝥注射液可抑制LoVo细胞的生长并诱导发生凋亡。

The chondroblast cultured with compound porous materials PDLLA and PDLLA/chitosan. The aim are: 1 To examine the proliferation ability and potential chondroblast differentiation; 2 To find an ideal condition stimulated BMSC differentiate into chondroblast; 3 To examine the chondroblast proliferation in porous scaffolds and to explore the interaction between cells and materials; 4 To examine the release of cytokine in vitro.

目的在于：（1）通过骨髓基质干细胞的原代分离、培养及成软骨诱导，观察原代细胞扩增能力及其向成软骨方向分化的潜能；（2）寻求骨髓基质干细胞向成软骨方向分化的理想诱导条件；（3）观察经成软骨诱导的MSC在PDLLA、PDLLA／壳聚糖多孔载体上三维的增殖状况，探求细胞与材料间的相互作用；（4）观察经成软骨诱导的MSC在体外三维培养系统中细胞因子的释放状况，了解维持诱导细胞软骨表型的合适条件及作用机制。

The aim are:lTo examine the proliferation ability and potential chondroblast differentiation ;2To find an ideal condition stimulated BMSC differentiate into chondroblast;3To examine the chondroblast proliferation in porous scaffolds and to explore the interaction between cells and materials;4To examine the release of cytokine in vitro.

取健康成人略后上棘处骨髓 5ml，经 percoll液分离后密度梯度离心，10'砌l密度接种培养，观察原代细胞的贴壁、增殖状况，细胞长满瓶底后进行传代培养；传至第三代细胞，重新消化后以500转／分钟轻度离心 5分钟，10V加 l接种，改用化学限定培养基代替完全培养基培养，倒置显微镜观察细胞生长情况及形态变化，甲苯胺蓝染色观察诱导细胞合成细胞外基质QCM）中的蛋白多糖，免疫细胞化学染色检测ECM中＊胶原的蛋白合成，RT干CR鉴定诱导细胞＊胶原mRNA的表达。

In T cells,TGF-beta1 can inhibit their proliferation,inhibit the differentiation of Th1 and Th2,inhibit the differentiation of CTL,induce the generation of regulatory T cellsTregin B cells,TGF-beta1 can inhibit their proliferation,induce IgA class switch,it also can induce the apoptosis of immature and resting B cells;in NK cells,TGF-beta1 can inhibit the secretion of IFN-γand the cytolytic function of NK cells.

对T细胞，TGF-beta1可以抑制其增殖，抑制Th1和Th2细胞的分化，抑制CTL的分化，诱导调节性T细胞的产生；对B细胞，TGF-beta1可以抑制B细胞的增殖，诱导IgA的类别转换，还可以诱导不成熟和静息B细胞的凋亡。

Adopting normal rat or mouse, chronic bacteria and nonbacteria prostatitis model, prostate proliferation model, and other corresponding model, and through seting up different control group, we performed laboratoty investigation to turn out weather qianlieqingshu capsule can cure chronic prostatitis, depress prostate proliferation, activate blood circulation and remove stasis, antixidant, defervesce and ease pain.

实验方法：采用正常大鼠或小鼠、慢性细菌性及非细菌性前列腺炎模型、前列腺增生模型以及其他相应模型的大鼠或小鼠，并通过设立不同的对照组以进行前列清疏胶囊对大鼠慢性前列腺炎的治疗作用、抑制前列腺增生、活血化瘀作用、抗炎作用、解热作用以及镇痛作用的实验研究。

The level of intense of proliferation of fetal lymphocyte was significantlyincreased in ICP group than those of normal control group in dermic soluble antigen induced lymphocyte proliferation reaction.

采用单向混合淋巴细胞反应法对照研究20例ICP患者及正常孕妇的胎儿淋巴细胞对母体外周血的单个核细胞，皮肤组织可溶性抗原，蜕膜组织可溶性抗原的增殖情况。

METHODS: A model of T cell activation and proliferation was established by stimulated the cells with Con A.T cells were treated with different concentrations of CPT. The expression of CD69, the early marker of CD3(superscript +) T cell activation, was measured by FACS. The proliferation index was determined by carboxyl fluorescin diacetate succinmidyl ester by flow cytometry. The cell-cycle distribution was analyzed by propidium iodide staining.

以ConA刺激小鼠T淋巴细胞，建立小鼠T淋巴细胞活化、增殖的模型，以不同浓度的CPT作用于该模型，流式细胞术检测T细胞早期活化标志CD69分子的表达；以活体染料羧基荧光素乙酰乙酸染色流式细胞术分析CPT在ConA刺激下小鼠淋巴细胞的增殖相关指数；以碘化丙啶染色流式细胞术分析细胞周期的分布情况。

Although translator has turned from being a crystal ball by which the original culture can unrestrainedly penetrate to another crystal ball by which the target culture can freely traverse, the translator's personal embodiment, in the process of cognitive act, are still absent in translation studies. Translators are still subjects without body or simply disembodied subjects.

译者虽然由原语文化可以自由穿透的玻璃球变成了译语文化可以自由穿越的玻璃球，但译者认知过程中的个体体验在翻译研究中依然缺席，译者依然仅仅是一个没有躯体体验的主体。

Chillingly, he claimed our technology is 'not nearly as sophisticated' as theirs and "had they been hostile", he warned 'we would be been gone by now'.

令人毛骨悚然的，他声称我们的技术是'并不那么复杂，像他们一样，和"如果他们敌意"，他警告说，'我们将现在已经过去了。