查询词典 proliferation

Because different cell lines may show various levels of susceptibility to adenovirus infection and virus production, non-selectively replicating wtAd5 was used to be a comparison with the tested conditionally replicating adenovirus CNHK500.ONYX-015, the first replication-competent adenovirus entered into clinical trials was also used to be a comparison. We used virus proliferation assay, cell viability assay and western blot to evaluate the proliferation and cytolysis selectivity of CNHK500. Results demonstrated that the CNHK500 virus proliferation multiples in breast cancer cell lines after 48 hours is similar to that of wtAd5, ONYX-015 virus proliferation ability is less than that of CNHK500 in cancer cells.

然后，通过与ONYX-015（E1B 55 KD a蛋白缺失的2型和5型嵌和型腺病毒，美国ONYX生化制药公司研制）、wtAd5进行对比，利用病毒增殖实验和细胞生长抑制实验，验证了CNHK500选择性增殖能力和肿瘤特异性杀伤作用；利用Western Blot检测CNHK500腺病毒E1A和E1B在乳腺癌细胞和正常成纤维细胞中的表达差异，揭示了腺病毒肿瘤靶向性的机制；利用携带绿色荧光蛋白的CNHK500-EGFP感染乳腺癌细胞株和正常成纤维细胞株，直观地观察其感染乳腺癌细胞的能力和在乳腺癌细胞中的增殖过程。

The study showed the recombinant wt/mCREG protein depressed the VSMC proliferation depending on dose and the optimal concentration was 400nM;2biologic function of CREG protein and the membrance receptor mechanism:①effect on VSMC migration: the wound healing experiment showed the OB2 cells migration was slower significantly after added wt/mCREG(400Nm) in supernatant. The HITASY cells migration were very slowly and no remarkable change. The gelatinase digestion and Western blot analysis showed the matrix metalloproteinase was decreased and TIMPs was increased;②effect on differentiation: after added wt/mCREG(400nM), the expression of myocardin, SMα-actin, MHC and caldesmin were increased and that of LM-1 and FN were decreased in OB2 cells. These effects were more significant when adding wtCREG.;③effect on VSMC proliferation: Cell cycle assay and BrDU stain showed: after added the wtCREG and mCREG protein, the ratio of cell in G0/G1 phase increased to 0.5773 and 0.5572 from 0.5308 respectively in OB2 group, which increased to 0.7369 and 0.7034 respectively from 0.6297 in HITASY group;3Role of M6P/IGF2R in CREG biologic function:①ELISA and co-immunoprecipitation showed the wt/mCREG binding to M6P/IGF2R directly.②antibody blocking test: when the anti-IGF2R was added to medium at the same time with wt/mCREG at different concentration(2μg/mL、4μg/mL、8μg/mL),the effects of CREG protein which depressing proliferation, migration, secretion and promoting differentiation were blocked, which had the positive correlation to the concentration of added anti body. The studies showed two combinant CREG promoted VSMC switch to differentiation phaenotype, at the same time, depress VSMC proliferation, migration and secreting extracellular matrix.

上述实验结果证实：两种重组CREG蛋白对VSMC增殖均有剂量依赖性的抑制作用，并且相同浓度的糖基化的CREG蛋白对细胞增殖的抑制效应更为显著，最佳效应浓度为400nM；2两种重组CREG蛋白添加后对HITASY和OB2细胞生物学行为的影响：①CREG蛋白对VSMC迁移的影响：刮伤实验发现，加入最佳效应浓度的wtCREG和mCREG蛋白24h后，OB2组迁移能力下降，HITASY组无明显变化；细胞外基质金属蛋白酶-2，9(Matrix metallo-proteinase 2，9，MMP2 ，9)明胶酶电泳检测和Western blot检测结果证实，两种CREG蛋白均可以使OB2细胞合成细胞外基质MMP2，9减少，而组织金属蛋白酶抑制物(Tissue Inhibitors of Metalloproteinases，TIMPs)增加；②CREG蛋白对VSMC分化的影响：加入400nM的wtCREG和mCREG蛋白12h后，OB2细胞myocardin、SMα-actin、MHC、caldesmin表达增加，LM-1、FN表达减少；③流式细胞仪分析细胞周期和BrDU染色分析证实，加入400nM的wtCREG和mCREG蛋白后，OB2组G0/G1期细胞由0.5308分别增加至0.5773和0.5572，HITASY组G0/G1期细胞由0.6297分别增加至0.7369和0.7034；3M6P/IGF2R在重组CREG蛋白的生物学功能中的调控作用：①免疫共沉淀和免疫荧光双染色分析结果显示，CREG蛋白与M6P/IGF2R存在直接结合；②应用抗体阻断实验：将不同浓度的anti- M6P/IGF2R(2、4、8μg /mL)与两种CREG蛋白同时加入培养液中，CREG蛋白抑制VSMC增值、迁移和合成细胞外基质、促进分化的效应减弱，而且与加入anti- M6P/IGF2R浓度正相关。

The company has the domestic and foreign advanced equipment, the craft and the stronger technical strength, and has many item of national level scientifie research achievement, the patent technology and three entir e automatic production 1 ines, At present goes on the market the principal variety has, in view of the diabetes paticent'' the new generation of Jinyu capsule, the Yuquan granular, the sealwort capsule, the Job's-tears capsule, the Chinese yam capsule''the preparation,''the multi-dimensional vein extends in view of the diabetes illness complicati on sickness patient ,the sugar full rice polishings''the high blood fats hypertension the multi-dimens ional capsule'' the mammmary gland proliferation''health care pastes in view of the mammary gland-proliferation disease, the rheumatism ossein proliferation disease''the ossein proliferation externally applies disperses''the chronic pharyngitis dis ease''XiaXingli the granular qranulew,for exprot adj ustment blood sugar product''Tarhucgranular and so on pure Chinese native medicine preparation.

公司拥有国内外先进的设备、工艺和较强的技术实力，并拥有多项国家级科研成果，专利技术和三条全自动生产线。目前上市的主要品种有，针对糖尿病患者的&新一代金玉胶囊、玉泉颗粒、黄精胶囊、薏苡仁胶囊、山药胶囊&制剂，针对糖尿病并发症患者的&多维脉络舒、尿足糠&高血脂高血压的&多维胶囊&针对乳腺增生疾病的&乳腺增生保健贴&，风湿病骨质增生疾病的&骨质增生外敷贴&慢性咽炎疾病的&夏星利咽颗粒&，供出口的调节血糖产品的&肽活颗粒&等纯中药制剂。

Effects of sinusoidally varying magnetic fields with one frequency of 2HZ and static magnetic fields with different strength of 10GS、200GS and 560GS on proliferation of osteoblasts had been observed. It had been found that PEMFs promoted proliferation of osteoblasts, static magnetic fields with intensities of 10GS and 200GS did not affect its proliferation whereas that of 560GS inhibited the proliferation.

通过观察频率为2HZ的正弦波电磁场及强度分别为10GS、200GS、560GS的静磁场对成骨细胞增殖的影响，结果显示：脉冲电磁场能显著促进成骨细胞的增殖；强度为10GS、200GS的静磁场对成骨细胞的增殖无明显影响，而强度为560GS的静磁场抑制成骨细胞的增殖。

The notable proliferation was not observed by eyes in the local of injection. The infiltration of inflammation cells and mild proliferation of fibrocyte around dura mater was observed by HE stained in 4 and 8 weeks after injection. Infiltration and exudation of inflammation cells was observed by HE stained in epidural nerve root. Compared with group A, no changes of group B, C and D were observed under specific stained. Proliferation of type Ⅱ collagen fibers around dura mater was seen under immunohistochemical stained in 4 and 8 weeks after injection. There is no significant demyelination changes under LFB stained. The thickness and shape of the myelin sheath in epidural nerve root was not regular under transmission electronic microscopy in 4 and 8 weeks after injection. Fibroblast was also seen there. In nerve endometrium, macrophage could be seen under TEM, myelinated nerve fiber changed significantly, but nonmyelinated nerve fiber changed mildly. When 8 weeks, the changes of group D is smaller than the group B and C.

给药局部肉眼观察未见明显的纤维组织增生；HE染色可见B、C、D三组给药后四周及八周时硬膜内外均有炎细胞浸润，纤维细胞轻度增生，硬膜外神经根内有炎细胞浸润及炎性渗出；特殊染色B、C、D三组同A组相比未见有脊髓及神经根的改变；免疫组化染色，给药后四周及八周时，硬膜内外均有Ⅱ型胶原纤维增生；固兰染色B、C、D三组未见有明显脱髓鞘改变，与A组相比无明显异常改变；电镜观察B、C、D三组在给药后的四周及八周时，表现为硬膜外神经根内髓鞘厚薄不一，形状不规则，可见成纤维细胞，神经内膜中可见有巨噬细胞；粗大的有髓神经纤维变化明显，无髓神经纤维受累较轻；八周时电镜下D组改变较B、C两组为轻。

The results indicated that HDPCs'proliferation was inhibited significantly when they were cultured in DMEM with 50、100μg/mlLPS. Low-dose of Chinese nutgall water extracts( 1.25、2.5、5μg/ml) resisted the inhibitory effect of LPS to HDPCs'proliferation significantly .From the third day , the proliferation activity of HDPCs incubated with 2.5μg/ml Chinese nutgall water extract and LPS increased signicificantly in a time-dependent manner.

结果显示，培养液中加入浓度为50μg/ml、100μg/ml的LPS时，HDPCs的增殖活性受到明显抑制，加入低浓度(1.25μg/ml、2.5μg/ml、5μg/ml)五倍子水提取物能明显拮抗100μg/ml的LPS对HDPCs增殖的抑制作用，2.5μg/ml五倍子水提取物拮抗100μg/ml的LPS对HDPCs增殖抑制作用第3天开始明显增加，并呈时间依赖性。

E ., carrgeenin gum 7 g/L, Ph 5.8-6.0 and light intensities 2000-3000 lx, were proper to the proliferation and development of axillary buds. The brown could he restrained effectively when the Vc(0.05 g/L.) was supplemented in the proliferation medium. The GA3 was not suitable for the proliferation of axillary buds of slash pine.

当卡拉胶为7 g/L、pH为5.8~6.0、光照度为2000~3000 lx时有利于湿地松丛生芽的增殖和生长；培养基中添加抗坏血酸0.05 g/L能有效抑制湿地松丛生芽增殖过程中褐化物质的产生，而赤霉素的添加则不利于湿地松丛生芽的增殖和生长。

30U、P30W、P30S1 and P30S2 were isolated from PIP30, and P60U、P60W、P60S1 and P60S2 were isolated from PIP60, and P1W、P1S1 and P1S2 were isolated from PIWE1-10, and P10W、P10S1 and P10S2 were isolated from PIWE10-100, and P100U、P100W、P100S1 and P100S2 were isolated from PIWE100 using DEAE Sepharose F. F. The proliferation rates of lymphocyte in vitro of each polysaccharide fractions obtained during the isolation and purification were detected in order to observe their immune activities. The results showed that crude polysaccharides both obtained from ethanol precipitation and ultrafiltrate had the promoting effects on lymphocyte proliferation in various degrees. P30U、P30W、P30S1、P30S2、P60U、P60W、P60S2、P1S1、P1S2、P10S1、P10S2、P100U、P100W、P100S1 and P100S2 had the promoting effects on lymphocyte proliferation.

IP30经阴离子交换层析(DEAE-Sepharose Fast Flow)得到分级多糖P30U、P30W、P30S1和P30S2，PIP60经阴离子交换层析得到分级多糖P60U、P60W、P60S1和P60S2，PIWE1-10经阴离子交换层析得到分级多糖P1W、P1S1和P1S2，PIWE10-100经阴离子交换层析得到分级多糖P10W、P10S1和P10S2，PIWE100经阴离子交换层析得到分级多糖P100U、P100W、P100S1和P100S2；采用体外刺激淋巴细胞增殖实验对离子柱分级分离的多糖进行活性实验表明P30U、P30W、P30S1、P30S2、P60U、P60W、P60S2、P1S1、P1S2、P10S1、P10S2、P100U、P100W、P100S1和P100S2是有活性的。P60W、P60S1、P1S1、P10S1和P100S1经凝胶柱(Sephacryl S系列材料)层析获得五个纯多糖组分P60w1、P60s1、P1SP1、P10SP1和P100SP1。

The purpose of our study is to investigate the mechanism of Cinnamyl aldehyde on NIH3T3 proliferation and collagen synthesis at an angle of cell biology. 1.Effects Of CA On NIH3T3 Proliferation And collagen synthesis (1) Cell proliferation was measured by MTT assay.

本研究以NIH3T3为细胞模型，从细胞生物学角度探讨肉桂主要有效成分桂皮醛的作用机制，为&回阳生肌法&提供理论依据，并为肉桂在中医外科治疗慢性皮肤溃疡过程中的推广应用提供药理学基础。

Methods: The model of evaluating T cell proliferation stimulated with polyclonal activators, ConA was established by vital dye carboxyl fluorescin diacetate succinmidyl ester labeling technique. The effect of a specific inhibitor for p38 MAPK, SB253580, on T cell proliferation on different doses and in different time was estimated by flow cytometry. At the same time, the percentage of proliferating T cells and proliferation index were measured by CellQuest and SPSS10.0 for Windows softwares.

以活体染料羧基荧光素乙酰乙酸琥珀酰亚胺酯染色，建立了在多克隆刺激剂刀豆蛋白A刺激下评价小鼠T细胞增殖的模型，通过流式细胞术分析p38丝裂原活化蛋白激酶的特异性抑制剂SB203580在不同剂量、不同时间对T细胞增殖的作用，并应用CellQuest和SPSS10.0 for Windows软件分析增殖细胞各代所占比例和增殖指数。

Although translator has turned from being a crystal ball by which the original culture can unrestrainedly penetrate to another crystal ball by which the target culture can freely traverse, the translator's personal embodiment, in the process of cognitive act, are still absent in translation studies. Translators are still subjects without body or simply disembodied subjects.

译者虽然由原语文化可以自由穿透的玻璃球变成了译语文化可以自由穿越的玻璃球，但译者认知过程中的个体体验在翻译研究中依然缺席，译者依然仅仅是一个没有躯体体验的主体。

Chillingly, he claimed our technology is 'not nearly as sophisticated' as theirs and "had they been hostile", he warned 'we would be been gone by now'.

令人毛骨悚然的，他声称我们的技术是'并不那么复杂，像他们一样，和"如果他们敌意"，他警告说，'我们将现在已经过去了。