查询词典 prokaryotic

Prokaryotic organisms sometimes considered a class or phylum or subkingdom; coextensive with the Cyanophyceae: cyanobacteria (blue-green algae).

有时被认为是一个纲或门的原核生物体；和蓝藻纲同域；蓝藻。

ABSTRACT］ObjectiveTo construct the prokaryotic expression vector of tegument protein pp85 of human herpesvirus 7 and to express it.

摘要］ 目的构建人疱疹病毒7型(HHV7)开放读码框（ORF U14的原核表达载体，并且进行原核表达。

KEY WORDS］human herpesvirus 7; tegument protein pp85; prokaryotic expression

关键词］疱疹病毒7型，人；被膜蛋白pp85；原核表达

Methods:The whole mature protein coding sequence of three truncated dystrophin gene was amplified by RT-PCR method applied to human muscle cDNA. The fragment was inserted into prokaryotic expression vector pET28a plasmid.

以人肌肉组织cDNA为模板，采用RT-PCR扩增三个截短的肌营养不良蛋白cDNA，分别克隆入原核表达载体pET28a中，经限制性内切酶双酶切及DNA序列分析鉴定目的基因。

In order to avoid prolin residue inhibiting enterokinase cleavage, 9bp of MGF cDNA 5′ end sequence was truncated by primer, then the obtained truncated MGF des(1-3MGF cDNA (321 bp) was subcloned in pET32a vector to construct a prokaryotic recombination expression plasmid.

通过RT-PCR从拉伸刺激的人成骨细胞中克隆MGF cDNA序列，并去除5'端9 bp的序列，使N端缺少对肠激酶(Enterokinase， EK)具有抑制作用的脯氨酸，将截短型MGF des(1-3 MGF cDNA序列克隆入pET32a质粒，构建重组表达质粒。

We adopted the codon usage of vaccinia virus to modified the codon usage of nef and expression them in two prokaryotic expression vector.

我们采用痘苗病毒总体密码子使用频率改造HIV的nef基因后，提高基因的AT含量，其对痘苗总体的CAI指数提高到0.9以上。

The purpose of the experiment is to realize the prokaryotic expression of β-glucosidase gene from Acetobacter xylinum ATCC 23769, which will be used to produce enzyme preparation so as to develop the efficiency of extracting resveratrol from plants. Aspergillus niger β-glucosidase gene is cloned both at DNA lever and cDNA lever, which will lay a foundation for further research about its expression in Saccharomyces cerevisiae in a secretory form with the aim of increasing resveratrol contents in wines.

本实验实现了木醋杆菌β-葡萄糖苷酶基因的原核表达，目的是为了生产粗酶制剂，用于提高从植物中提取白藜芦醇的得率；分别在DNA和cDNA水平从黑曲霉中克隆β-葡萄糖苷酶基因，为下一步在酿酒酵母中实现分泌表达以提高葡萄酒的白藜芦醇含量的研究奠定基础。

Vitreoscilla hemoglobin is the only well characterized prokaryotic oxygen-binding protein that allows bacterium to grow aerobically even under poor oxygen conditions.

透明颤菌血红蛋白（Vitreoscilla hemoglobin， VHb）是唯一一种研究得较为透彻的原核生物氧结合蛋白血红蛋白。

Neuroglobin, a recently identified monomer globin in nerve system, is highly affinitive with oxygen similar to myoglobin. After the coding region of rat NGB gene was sub cloned into the prokaryotic expression vector pGEX 4T 2, the recombinant vector was introduced into E. coli BL21 (DE3) cells for efficient expression.

氧是人类及许多生物赖以生存的重要条件，严重缺氧和低氧可危及人体生命。2 0 0 0年 9月，德国Burmester等人首次报道了人和小鼠脑内存在特异的功能上类似于肌红蛋白的携氧蛋白———脑红蛋白(neuroglobin ，NGB) [1 ] 。

Transgenic plants expressing GNA showed significant insecticidal activity towards \\omoptera insects such as aphids and brown planthopper in bioassay and feeding tests.In this paper a lectin gene was firstly isolated from leaves of Zephyranthes grandiflora, belonging to Amaryllidaceae, by the use of rapid amplification of cDNA ends technique. Characterization, functional prediction and construction of plant and prokaryotic expression vector of this novel gene were studied.

本实验采用cDNA末端快速扩增(Rapid amplification of eDNA ends，RACE)技术，首次从中国特有的野生植物韭莲(又名风雨花，Zephyranthes grandiflora)的叶片中分离克隆出了一个新的凝集素基因，对基因进行了序列分析和功能预测，并成功地构建了韭莲凝集素基因的植物表达载体和原核表达载体。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新