查询词典 prokaryotic

With regard to the rapidly increasing sequence data of prokaryotic genomes, accurate gene identifications and genome annotations have become more and more important.

近年来原核生物的基因体定序速度越来越快，相对地基因的鉴定以及基因的注解也变的越来越重要。

The prokaryotic expression vector pET22b and host bacterium Roestta-gami are most suitable for CM-VEGF121,which solves the problem of low expression and insolubility.

原核表达载体pET22b和Rosetta-gami宿主菌为CM-VEGF121最合适的条件，解决了其表达量低和不可溶问题。

After prokaryotic expression, fusion protein existed in sediment and accounted for 56% of all bacterial lysate.

结果： 经酶切鉴定、序列测定证实融合基因已插入pET28a载体中，融合基因序列与设计序列完全一致。

So it can be concluded that prokaryotic canine lysozyme obtained in this experiment had potential application value.

表明本试验获得了有活性的、具有一定潜在应用价值的犬溶菌酶。

Objective To construct the prokaryotic coexpression vector for lysis gene E and Staphylococcus nuclease gene and prepare highly purified E.

目的 构建噬菌体裂解基因E和核酸酶基因串联表达载体，制备高质量的大肠杆菌菌影。

Methods The total RNA was extracted from the brain of human embryo. The segment of open read frame of DOC-1 was amplified by RT-PCR and was further cloned into the vector, pMD18-T, and the prokaryotic expression vector, pGEX-4T-1, by turns, to produce the new construct pGEX-4T-1-DOC-1. After restriction enzymes digestion analysis and sequenced, pGEX-4T-1-DOC-1 was transformed into E.coli BL21（DE3）. GST-p12 recombinant protein was expressed under IPTG induction and purified by affinity column.

从人胎脑组织中提取总RNA，经逆转录-聚合酶链式反应扩增DOC-1的CDS序列，再通过基因重组技术将该基因片段依次克隆到pMD18-T和pGEX-4T-1载体中，构建融合表达载体pGEX-4T-1-DOC-1，经酶切、测序鉴定后，用该重组质粒转化E.coliBL21，用IPTG诱导表达，Glutathione Sepharose 4B柱亲和层析纯化重组蛋白。

Producing PHAs,and then were orientationally inserted into a prokaryotic expression vector pBV220. By analyzing the bioactibity and function of their products, the biological function of phaA, phaB and phaC was confirmed. These have been done in our laboratory. To make better use of the three genes by plant bioreactor, two groups of different plant expression vectors that can be used to produce PHAs in different plants were constructed, and the transformation of PHA in Oxalis triangnlaris has been preliminarily reseached.The following is the main results:Three plant expression vectors pBI121-A,pBI121-B and ubquiting-C, which can be used to be transformed into Oxalis triangularis were respectively constructed by using the phaA, phaB and phaC.

菌株的亚克隆基因组片段中，分离出phaA、phaB和phaC三个基因片段，定向克隆至原核表达载体pBV220，通过对其生物活性与功能分析，确证了基因phaA、phaB和phaC的生物学功能；为了通过植物生物反应器对这三个基因进一步研究利用，本实验利用这三个基因构建了两组可用于不同植物生产PHA的真核表达载体，并对紫叶酢浆草进行PHA基因遗传转化的初步研究，获得主要结果如下：利用克隆的phaA、phaB、phaC三个基因分别构建了可用于转化紫叶酢浆草表达载体pBI121-A、pBI121-B、uBquiting-C；可用于玉米转化的种子特异性表达载体p7S-A、pBI121-S-B、uBquiting-C。

OBJECTIVE: To construct a prokaryotic expression plasmid of the main catalytic domain of dipeptidyl peptidase of murine fibroblast activation protein αand obtain the target fusion protein in order to provide basis for production of antibodies and anti-tumor vaccine targeting FAPα.

目的：构建小鼠成纤维细胞激活蛋白α二肽基肽酶催化核心序列的高效原核表达载体，进行该序列的原核表达、纯化和复性，为下一步高效抗体制作和以FAPα为靶标的抗肿瘤疫苗研发提供实验基础。

Objective To construct a prokaryotic expression vector for LY1 protein phosphatase gene, and to express the gene in E.

目的 构建蛋白磷酸酶LY1基因的原核表达载体，并在E。

In the current study, a series of cationic α-helical peptides of 20 a.a. were designed and synthesized based on four structural parameters, including charge, polar angle, hydrophobicity, and hydrophobic moment. Several methods were performed to describe the influence of these parameters on the antimicrobial and hemolytic activity, and on the permeabilizing effect on model membranes. The potential of these parameters to increase the antibacterial activity and to improve the prokaryotic selectivity is assessed.

本实验利用自行设计合成的人工抗菌胜肽当模型，固定长度为 20 个胺基酸，改变其电荷、极性角度、疏水性及疏水性矩等四种结构特性，并利用其对各种不同病原菌之抗菌活性及对兔子红血球细胞的溶血活性来研究其结构特性的改变对其抗菌活性、选择性及微脂体膜的渗透性之影响，以期望改变结构参数能增加抗菌活性及对原核的选择性。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新