查询词典 prokaryotic

Accordingly, eukaryotic microorganism cells would die. In the experiment, Staphylococcus aureus was employed as the representative of prokaryotic microorganisms, and Candida albicans and Trichophyton mentagrophytes as the representative of eukaryotic microorganisms, respectively.

在实验中，作为原核微生物的代表使用金黄色葡萄球菌，作为真核微生物的代表使用了白色念珠菌和须癣毛癣菌，并对各种类进行了检测对比。

Chicken IL-15(ChIL-15), Which was discovered in 2001, plays a main role in activating NK cells and CD8+ memory T cells, and may therefore have potential as a vaccine adjuvant.According to published ChIL-15 gene sequence, a pair of primers were designed and synthesized to clone ChIL-15 cDNA from ConA-activated chicken splenocytes by RT-PCR. Recombinant plasmid pUC19ChIL-15 carrying the ChIL-15 gene was constructed. The gene encoding mature ChIL-15 (mChIL-15) was amplified from pUC19ChIL-15 by PCR and cloned into pMD 18-T vector. After digested with Kpn I /Pst I , the mChIL-15 gene was subcloned into prokaryotic expression vector pPRoEX橦Ta and transformed into E.coli DH5a .The transformant was induced by IPTG, and the recombinant ChIL-15(rChIL-15) was expressed as a fusion protein with a histidine hexamer tag at the N-terminal end of the protein. Following expression, the protein was purified by ProBond?Nickel-Chelating Resin. The uninduced and induced protein lysates and the purified protein were separated by SDS-PAGE and transferred onto nitrocellulose membrane to identify rChIL-15 by Western blot. The rChIL-15 was used to immune the guinea pig to obtain rChIL-15 polyclonal antibody.

参考已发表的ChIL-15基因序列，设计合成引物，应用RT-PCR技术从刀豆球蛋白A活化的白来航鸡脾淋巴细胞中克隆ChIL-15 cDNA，将其与SmalⅠ酶切处理的pUC19载体连接，构建重组质粒pUC19ChIL-15；用PCR技术从pUC19ChIL-15中扩增出去信号肽的成熟ChIL-15(mChIL-15)基因，与pMD 18-T载体相连构建重组质粒pMDChIL-15；然后用KpnⅠ／PstⅠ双酶切下mChIL-15基因片段，定向亚克隆到经同样酶切处理的带有6个组氨酸标签的表达载体pP_RoEX~HTa中，构建重组质粒pP_RoChIL-15，对其测序确认读框正确后，转入大肠杆菌DH5α中诱导表达并进行纯化，用重组ChIL-15(rChIL-15)免疫豚鼠，制备多克隆抗体；再用HindⅢ／PstⅠ从质粒pP_RoChIL-15上切下mChIL-15基因，定向亚克隆到经同样酶切处理的杆状病毒转移载体pMelBacB中，经酶切、PCR和序列测定鉴定后，与杆状病毒线形化DNABac-N-Blue~(TM DNA共转染Sf9昆虫细胞，经三轮蚀斑纯化，构建重组杆状病毒rBac-ChIL-15，用该重组病毒感染处于对数生长期的Sf9细胞，按不同的时间收取样品，离心后对其上清和沉淀进行SDS-PAGE和Western blot分析。

There was still some hope for anyone who wished to uphold the simple, prokaryotic picture of unsplit genes.

对于那些希望坚持基因都象原核生物那样不可分裂的简单理论的人来说，仍然存在着一线希望。

Objective To perform prokaryotic expression, purification and identification of Xenopus laevis and mouse vascular endothelial growth factors.

目的 构建非洲爪蟾和小鼠血管内皮细胞生长因子重组表达载体，进行蛋白质原核表达，并对表达产物进行分离、纯化和鉴定。

Antiserums against the coat proteins were prepared by prokaryotic expression and 3 by virus purification.

原核表达制备了27种线状植物病毒的外壳蛋白抗血清，提纯病毒制备了3种线状植物病毒的外壳蛋白抗血清。

Antiserums against the non-structural proteins of those viruses were also prepared by prokaryotic expression.

原核表达制备了24个功能蛋白的抗血清。

Using Perl as programming language and integrating databases technology, microsatellites, longer than 12 nucleotides in length, in 29 eukaryotic and 1180 prokaryotic genomes were computed and censused in great scale.

本研究采用Perl为编程语言结合数据库技术等方法，对29个真核生物基因组和1180个原核生物基因组的DNA序列上长度超过12的微卫星进行了大规模的计算和统计分析。

In this study, we cloned the BFV gag gene into prokaryotic expression vector pET28a and purified the denaturalized Gag protein. The protein was used to immunize BALB/c mouse to produce antiserum, which could specifically recognize the BFV Gag protein in BFV-infected cells through western blot assay.

本文将BFV gag基因克隆入原核表达载体pET28a中，纯化得到了变性的Gag蛋白，用于免疫BALb/c小鼠获得抗血清，进行了Western blot杂交和免疫荧光实验。

The gut of lower termites harbor a complex microbial community,including eukaryotic flagellates and prokaryotic bacteria and archaea,which play important roles in the wood-cellulose digestion of these termites.

低等白蚁肠道里存在着复杂的微生物区系，包括真核微生物鞭毛虫和原核生物，细菌及古细菌。

Swine hemagglutinating encephalomyelitis virus ; Hemagglutinin esterase protein ; Cloning ; Prokaryotic expression

猪血凝性脑脊髓炎病毒；血凝素酯酶蛋白；克隆；原核表达

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新