查询词典 prokaryotic

Objective To construct an expression vector for fusion gene of heat-liable enterotoxin B subunit and major outer membrane protein of Chlamydia psittaci and express the fusion gene in prokaryotic cells.

目的构建含大肠杆菌热不稳定肠毒素B亚单位和鹦鹉热衣原体主要外膜蛋白融合基因的表达载体，并在原核细胞中表达融合蛋白。

Chlamydia psittaci ; Escherichia coli heat-liable enterotoxin B subunit ; Major outer membrane protein ; Prokaryotic expression

鹦鹉热衣原体；大肠杆菌热不稳定肠毒素B亚单位；主要外膜蛋白；原核表达

Objective: To construct the prokaryotic expression vector of human cancer-testis gene NY-ESO-1 by the method of LP Recco PCR Cloning.

目的：利用PCR产物靶向克隆法构建人癌－睾丸抗原NY-ESO-1的原核表达载体。

LP Recco PCR Cloning can efficiently and conveniently construct high quality prokaryotic expression vector of human NY-ESO-1 gene, which provide the expression vector to produce pET-15b-NY-ESO-1 fusion protein.

靶向克隆法可快速、高效地构建人NY-ESO-1基因的原核表达载体，为制备pET-15b-NY-ESO-1融合蛋白奠定了基础。

But 5-helix was apt to form inclusion body when expressed directly in prokaryotic cell and was difficult to renature, which causes inconvenience to future study.

但5-helix基因在原核细胞中直接表达时易形成包涵体，复性困难，给研究带来不便。

Two relevant sites of enzymatic digestion were added to the mTNF-α by PCR. The mTNF-α was linked to the 3'end of m/〓 in pGEX4T-1 vector. The prokaryotic expression vector pGEX4T-1m/〓-mTNF-α was constructed successfully. After induction and expression by IPTG, the expression of two kinds of fusion protein is 15% and 12% of total bacteria proteins respectively. The anti-HCC bifunctional antibodies m/〓-mTNF-α were identified by electrophoresis after the inclusion bodies were purified, denature, renature, re-purified, digested by thrombin and further purified.

采用PCR的方法在mTNF-α的两端加上所需要的酶切位点，将之连接在m／〓的3'端，构建原核表达载体pGEX4T-1 m／〓-mTNF-α，通过IPTG的诱导表达之后，两种融合蛋白的表达量分别占细菌总蛋白的15％、12％，表达产物经包涵体的纯化→变性→复性→纯化→凝血酶酶切→进一步纯化后，可以得到纯度为电泳纯的m／〓-mTNF-α抗肝癌双功能抗体。

Prokaryotic expression vector pET28b㈩/ TprK has been constructed successfully, and pET28b㈩/ TprK protein expressed to have set foundation for preparing prophylactic vaccine and developing serologically diagnostic kit for TP infection.

成功构建人pET28b㈩/TprK原核表达载体，并能高效表达TprK蛋白，为梅毒的预防性疫苗的制备以及进一步研究其血清学诊断试剂奠定了基础。

Another pair of primers including the start codern and the terminer coden were designed to amplify the PAL gene. Two restriction enzyme XbaI and XmaI were added to the two primers respectively, which enable to insert this fragment to the prokaryotic expression vetor pCAMBIA2301-WCS.

在PAL 基因的编码框以启始密码子ATG 和终止密码子TAG 为两端设计一对特异引物，分别在引物5' 端引人XbaI 和XmaI 切位点，用来扩增PAL 基因并与pCAMBIA2301-WCS 构建表达载体pCAMBIA2301-PAL。

Another pair of primers including the start codern and the terminer coden were designed to amplify the PAL gene. Two restriction enzyme XbaI and XmaI were added to the two primers respectively, which enable to insert this fragment to the prokaryotic expression vetor pCAMBIA2301-WCS.

在PAL 基因的编码框以启始密码子ATG 和终止密码子TAG 为两端设计一对特异引物，分别在引物5' 端引人XbaI 和XmaI 酶切位点，用来扩增PAL 基因并与pCAMBIA2301-WCS 构建表达载体pCAMBIA2301-PAL。

To clone the site A of S gene of transmissible gastroenteritis virus and to construct the prokaryotic expression vectors of site A.

对猪传染性胃肠炎病毒S基因A抗原位点进行克隆和原核表达载体的构建。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新