查询词典 projecting microscope

Results:Whith the time and doses of ART incubation extended, ART significantly inhibited the proliferation of SGC-7901 cells and the inhibited effect shows dose-and -time-dependent. Obvious changes of apoptotic morphology were observed by invert microscope, fluorescence microscope, scanning electron microscope and transmission electron microscope, they showed that cells had marked nuclear condensation or the fragmentation of chromation as well as apoptotic , mitochondrial edema and vesicle , with the time of incubation extended , the proliferation rate become slow and the volume became small and transformed. FCM assay indicated that most of the cells were arrested in Go/Gi and the apoptotic peak appeared. During the prolong of incubation time, the apoptosis rate was increased. At the same time, the number of S and G2/M phase cells were decreased. The result of TUNEL indicant that there are apoptosis and necrosis.

结果：随着药物浓度的增加和作用时间的延长，蒿甲醚对胃癌SGC-7901细胞的抑制作用呈时间和浓度依赖关系：在倒置显微镜、荧光显微镜、扫描电镜和透射电镜下可观察到典型的凋亡细胞的形态改变，表现为：核固缩或染色质边聚或凝聚成大块状，可见凋亡小体，线粒体肿胀增殖，严重的空泡化，随作用时间的延长，细胞增殖速度减慢，细胞体积缩小变形；流式细胞术显示胃癌SGC-7901细胞出现明显的凋亡峰，随着作用时间的延长，其凋亡率逐渐升高，细胞周期阻滞在G_0/G_1期，S及G_2/M期细胞数大量减少；流式细胞术TUNEL检测结果显示细胞凋亡和坏死同时存在。

Results (1) CCD fluorescent microscope was superior to laser scanning confocal microscope in collecting the fluorescent image of DCF. CCD fluorescent microscope was chosen to be the system applied in this research with the consideration of operation, cost and et al. The mercury-arc lamp of fluorescent microscope was fit for the requirement of our study. The wavelength range of the light output was 460-490 nm and the power density was about 100mW/cm in the condition of our study. Organelle-cell fluorescence intensity ratio analysis was more suitable than other methods.

结果：(1)CCD荧光显微镜采集到的DCF荧光图像的质量优于共聚焦显微镜，同时综合考虑包括采图质量、光照射剂量的可控性、实验成本和操作简便性等诸多因素，最终确定了CCD荧光显微镜作为实验研究的工具；荧光军医进修学院硕士学位论文中文摘要显微镜的汞灯输出功率稳定，光斑均匀，经本实验所选取的光路系统后输出波长范围为460一490nm，功率密度约为1 00 mw/cm；细胞器一细胞荧光强度比值法对于荧光分布位置的确定优于其他方法。

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可程式恒温恒湿箱、高低温试验箱、盐水喷雾试验机、干燥箱、热老化试验机、耐寒/耐黄变试验箱、冷热冲击试验机、灼热丝试验机、垂直+水平燃烧试验机、漏电起痕试验机、按键寿命试验机、插头插座负载寿命试验机、弹簧试验机、90度剥离强度试验机、破裂强度试验机、环压强度试验机、纸管/纸箱抗压试验机、耐磨擦试验机、插拔力试验机、转轴寿命耐久试验机、手机翻盖寿命试验机、鼠标滚轴寿命试验机、振动试验机、模拟汽车运输振动台、手机跌落试验机、包装跌落试验机、电线曲挠试验机、插头线突拉试验机、静态拉力试验机、线材弯折摇摆试验机、线材伸长率试验机、万能材料试验机、拉力试验机、落球冲击试验机、耳机疲劳寿命试验机、耳机拉伸寿命试验机、推拉力计、布、洛、维、肖、里、邵氏硬度计、影像测量仪、精密测绘显微镜、数字投影仪、体视显微镜、金相显微镜、读数显微镜、连续变倍显微镜、放大镜、卡尺、千分尺、标准砝码、高度计、厚度计、岩石平板、电子计重称、电子计数称、电子天平、分析天平、标准光源箱等。

Get high purity DCs by Cultured plastic-adherent monocytes isolated from healthy human peripheral blood with GM-CSF and IL-4 for 7 days. To observe the morphology of DCs by inverted phase contrast microscope ,electron microscope and laser confocal microscope. Analyse phenotype of DCs with flow cytometry. Investigate the endocytosis ability of DCs as a group by Horseradish peroxidase endocytosis assay. To appraise allogeneic mixed lymphocytes reaction of DCs by MTT reduction assay. Analyse the levels of IL-12 and TNF in liquids of cultured medium by ELISA and MTT reduction assay respectively. Soluble antigens of HCCs was obtained by 3 freeze-and-thaw cycles. Biological characteristics of HC soluble antigens pulsed DCs were monitored by flow cytometry. According to MTT reduction assay estimated the cell proliferation of self lymphocytes activated by HC antigens pulsed DCs. Get high purity BCG HSP 70 protein by SDS-PAGE electrophoresis and determined its biological activity with ELISA. Analyse phenotype of antigen pulsed DCs primed by BCG HSP70 with flow cytometry. By MTT reduction assay estimated the cell proliferation of self lymphocytes and the MLR of DC based vaccine. Analyse expression of HLA-DR molecule on surface of HCC lines. The IFN-γ mRNA in lymphocytes after actived by DC vaccine and the Fas-L expression on DC and DC vaccine primed lymphocytes were detected by in situ hybridization and flow cytometry respectively. Specific cytotoxity lysis of T lymphocytes and nonspecific inhibition of liquids in culture medium against HCC lines were also tested. Detect expression of hAFP on four HCC lines with Cell-ELISA. Induce apoptosis of HCCs with actinomycin-D. Interaction of DCs and apoptotic cells was observed under transmission electron microscope. Growth inhibition test of DC against HCC lines was also performed. Establish the nude mouse model bearing human HC xenografts and indentify the characteristic of tumour by histochemistry and immunohistochemistry techniques. Prevent and treat transplanted human HC on nude mouse with Freezing and anabiotic HC specific lymphocytes.

用GM-CSF和IL-4从健康人外周血诱导DC；分别用倒置相差显微镜、电子显微镜及激光共聚焦显微镜观察DC形态；流式细胞术检测DC表型；HRP吞噬实验测定DC的群体内吞能力；MTT法检测同种异体混合淋巴细胞反应；ELISA法和MTT法分别测定DC培养上清液中IL-12和TNF水平；冻融法制备肝癌细胞可溶性抗原；流式细胞术检测负载肝癌可溶性抗原后DC的生物学特性；MTT法检测DC负载肝癌抗原后对自身淋巴细胞增殖的影响；SDS-PAGE制备电泳纯化BCG HSP70并鉴定纯度，ELISA测定活性；流式细胞术检测负载抗原DC经BCGHSP 70活化后的表型；MTT法检测肝癌DC疫苗对自身淋巴细胞增殖的影响和混合淋巴细胞反应；流式细胞术检测肝癌细胞表面HLA-DR表达；MTT法检测肝癌DC疫苗对自身淋巴细胞的活化；原位杂交法检测肝癌DC疫苗活化后的淋巴细胞IFN-γmRNA表达；流式细胞术检测DC和肝癌DC疫苗活化后淋巴细胞表面Fas-L；MTT法分别检测肝癌DC疫苗活化的淋巴细胞和其培养上清对肝癌细胞的特异性杀伤和非特异性抑制作用；Cell-ELISA检测人肝癌细胞hAFP表达；MTT法检测负载AFP表位肽和凋亡肝癌细胞DC对自身淋巴细胞增殖的影响；ELISA法和MTT法分别测定活化后淋巴细胞培养上清中TNF和IL-12水平；肝癌细胞凋亡的诱导和检测；DC吞噬凋亡肝癌细胞后的电子显微镜观察；DC对肝癌细胞的生长抑制试验；人肝癌裸鼠皮下移植瘤动物模型的建立及其组织学和免疫组织化学鉴定；DC及肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤；冻存和复苏后的肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤。

Results The according perception of Normocytic, Microcytic and Non-classified RBC between phase contrast microscope and UF-100 urine sediment analytic instrument RBC-info are 91.4%, 94.4%, 83.3% respectively, the according perception between phase contrast microscope and RBC-P70Fsc are 94.9%, 95.7%, 94.7% respectively, and the according perception between phase contrast microscope and RBC Fsc-DW are 84.4%, 86.8%, 90.5% respectively, the specificity of UF-100 and phase contrast microscope in glomerular hematuria and non-glomerular hematuria are 84.3%, 88.1% and 83.3%, 87.9% respectively.

结果 观察相差显微镜与流式细胞分析仪RBC-info尿红细胞均一型、变异型、混合型形态符合率分别为91.4%、94.4%、83.3%，与RBC-70Fsc符合率分别是94.9%、95.7%、94.7%，与RBC-Fsc-DW符合率分别是84.4%、86.8%、90.5%，流式细胞分析仪与相差显微镜的特异性在肾小球性血尿和非肾小球性血尿分别是84.3%、88.1%和83.3%、87.9%。

The morphology and structure of reconstructed tissue was detected by microscope and scanning electron microscope.Results:(1) Compared with the control group, the cellular proportion of laminin group increased in 62 ~M phase, and decreased in Go~Gi phase significantly. As shown by the microscope, the cells of control group were in low density. The cells in mass connected tightly. The microfilament appeared reticular formation. The nucleus were the same in size. The cells of laminin group were in high density and put out so many lamellipodia, filopodia, which connected with the surrounding cells. The microfilament increased, elongated, and changed from reticulodromous to sarciniform, which reached to the pseudopods. The nucleus were different in size .(2) As shown by the inverted microscope and the cell growth curve, comparing with the controlgroup, cells of each test group increased evidently. The cellular proportion of each test group increased in S phase and G2 ~M phase, and decreased in Go~Gi phase significantly, but there was no considerable interations between LN and EGF;(3) As shown by the morphological observations, the cultured cat corneal endothelial cells formed an integrated membrane, and attached to the Descemets membrane closely, which was similar to the natural tissue. The cells connected tightly to each other, and some of them arranged in hexagon approximately.

结果：(1)层粘连蛋白组处于G_2～M期细胞比例较对照组显著提高，Go～G_1期细胞比例显著下降，提示层粘连蛋白促进内皮细胞DNA合成，及细胞分裂增殖；光镜下，对照组细胞分布成团状，细胞密度较低，细胞间连接紧密，细胞内微丝结成网状，细胞核大小一致；与对照组相比，层粘连蛋白组细胞生长旺盛，细胞密度高，向周边伸出大量板状及丝状伪足，细胞内微丝增多、拉长、集结成束，伸入伪足中，细胞核形状大小不一致；(2)倒置显微镜观察及细胞生长曲线显示，各组细胞数目随时间增加而明显增多，各实验组较对照组增生显著，EGF和LN联合应用组各时间点细胞数目最高；实验组处于S期和G_2～M期细胞数目增加，Go～G_1期细胞数目减少；提示EGF、LN单独及联合应用均可促进细胞增殖，但尚不能认为二者有交互作用；(3)倒置显微镜下，组织培养的猫角膜内皮细胞排列成密集的单层，细胞间连接紧密；组织学观察发现，培养的猫角膜内皮细胞形成完整的内皮层，贴附于脱水基质的后弹力膜上，与正常的角膜内皮组织结构相似；扫描电镜下，组织培养的猫角膜内皮细胞间紧密镶嵌排列，可见某些细胞呈近似六边形排列，细胞大小不甚一致，胞核清晰。

Management content: The company product mainly covers the microscope, the tool maker's microscope, the projecting apparatus, the sclerometer, the thickness gauge, the magnifying glass, the balance, the measuring instrument quantity meter, the mapping equipment, the metering equipment, to test the instrument, the electrochemistry instrument, the physical instrument, the analytic instrument, the biochemistry instrument, the non-destructive inspection, the medical instrument, the processing machinery and so on altogether 28 broad headings, 170 minor sort large-scale specialized precision instrument measuring appliance sale enterprises.

经营内容：公司产品主要涵盖显微镜、工具显微镜、投影仪、硬度计、测厚仪、放大镜、天平、量具量仪、测绘仪器、测量仪器、实验仪器、电化学仪器、物理仪器、分析仪器、生化仪器、无损检测、医疗器械、加工机械等共28大类，170余个小类的大型专业精密仪器仪表价格企业。

The Corporation product covering microscope, the tool maker's microscope, the projecting apparatus, the sclerometer, the thickness gauge, the magnifying glass, the balance, the measuring instrument quantity meter, the mapping equipment, the metering equipment, test the instrument, the electrochemistry instrument, the physical instrument, the analytic instrument, the biochemistry instrument, the non-destructive inspection, the medical instrument, to process the machinery altogether 18 broad headings, 170 minor sort.

公司产品涵盖显微镜、工具显微镜、投影仪、硬度计、测厚仪、放大镜、天平、量具量仪、测绘仪器、测量仪器、实验仪器、电化学仪器、物理仪器、分析仪器、生化仪器、无损检测、医疗器械、加工机械共18大类，170余个小类。

This paper investigates the converse theorem of rectangular projection theorem in descriptive geometry,and furthermore supplements and confirms another converse theorem,If the projection of two lines intersecting vertically fo rms a rectangle on a certain projecting plane,at least one line of the two lines is parallel to the projecting plane.

通过对《画法几何学》中直角投影定理的逆定理的研究，得出："若垂直相交的两直线在某一投影面上投影成直角，则该两直线至少有一条直线平行于该投影面"的推论。

In subspace projecting point of view, chapter 8 considered a transient detecting scheme base upon matching projecting on the linear time-frequency subspace of observing space, and gave a fast implementation way of matching filter for two type of signals, together with its performance analysis.

第八章从子空间匹配投影的角度研究了一种基于线性时频变换信号子空间匹配投影的暂态信号检测方案，推导了线性时频域上两种信号的匹配检测器的快速实现形式并进行了性能分析。

Summary In copyright infringement cases,if the alleged infringer uses or even plagiarizes the copyrighted works or parts thereof,which causes no substantially adverse effect on the normal exploitation of the work,nor does it inflict any substantial damages to the right owner,the de minis doctrine should be applied to exempt the liabilities of the defendant and seek the balance of interests between the right owner and the general public embodied by the copyright law.

在侵犯著作权案件中，如果被控侵权行为人虽未经著作权人的同意，使用甚至抄袭了受著作权保护的作品或作品的片段，但若情节轻微，未对该作品的正常使用产生任何实质不利的影响，亦未对权利人的权利造成实质性的损害，则应适用&不计琐细原则&，免除行为人的责任，以寻求在权利人利益与著作权法所体现的社会公共利益之间的平衡。

This new product will become our best seller.

这个新产品会成为我们最畅销的产品。