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Based on the previous literatures, the following major innovation works were carried out in this dissertation:(1) The derivatization reaction conditions of ephedrine and pseudoephedrine with sensitive derivation reagent in aqueous system were studied and two new methods were developed for the assay of them by microemulsion electrokinetic chromatography and micellar electrokinetic chromatography with LIF detection. The sensitivity and analysis times were greatly improved compared with the previous reports;(2) A new method of CZE with indirect LIF detection was developed for the simultaneous determination of six coumarin compounds (esculin, esculetin, isofraxidin, genistein, naringin and sophoricoside) with fluorescein as the probe. The proposed method enlarges the application range of LIF detector, and provides new approach for the analysis of certain compounds difficult to derivatize;(3) Based on the above research, an MEKC with indirect LIF detection method for the simultaneous determination of adenine and guanine in DNA extracts from fungus, maize and soybean was established;(4) A new method for the investigation of the complexes formed between human serum albumin and ampicillin sodium under the simulated physiology conditions using laser light scattering technique was developed.

该论文在综述前人工作的基础上,开展了如下未见文献报道的创新性的研究工作:(1)使用灵敏的衍生试剂对麻黄碱和伪麻黄碱在水体系中的衍生反应条件进行了系统研究,建立了微乳电动色谱-激光诱导荧光检测法和胶束电动色谱—激光诱导荧光检测法测定麻黄碱和伪麻黄碱的灵敏分析新方法,与以前的报道相比,灵敏度和分析时间均有很大改善;(2)使用荧光素钠作为背景荧光试剂,建立了同时分析测定六种黄酮类化合物(秦皮甲素、秦皮乙素、异秦皮定、染料木素、柚皮苷和槐角苷)的毛细管区带电泳—间接激光诱导荧光检测新方法,扩大了激光诱导荧光检测的应用范围,对难衍生化合物的分离分析提供了一种新思路和新途径;(3)以荧光素钠作为背景荧光试剂,建立了一种用于同时测定食品提取DNA中的腺嘌呤和鸟嘌呤含量的胶束电动色谱—间接激光诱导荧光检测新方法;(4)用动态和静态激光散射法研究了模拟生理条件下人血清白蛋白与氨比西林钠盐相互作用所形成的复合物,为药物分子与蛋白质的相互作用、药代动力学等的研究提供了一种新思路。

A set of ICP etching system has been designed and manufactured through the analysis of the probe diagnosed results.

这些结论为设计ICP刻蚀系统的反应室、选择合适的刻蚀工艺提供了科学的根据。

Given banks are huge players in the foreign-exchange market, any evenhanded probe would have to involve them, too.

鉴于银行是外汇市场上的重量级参与者,任何公正无私的调查应该也针对它们才对。

OBJECTIVE: To observe the effects of 2-CAdo adenosine-receptor excitant on genetic expression of bcl-2, Bax of hippocampal cells in epileptic rats and further probe into the mechanism of adenosine on anti-convulsion and brain protection.

目的:观察腺苷受体激动剂2-CAdo对癫痫大鼠海马神经细胞bcl-2,Bax基因表达的影响,进一步探讨腺苷抗惊厥及脑保护的作用机制。

From pump-probe experiments results, we can identifythat the excited state absorption can be neglected in this kind of compounds.

泵浦-探测实验结果表明,这些化合物的激发态吸收可以忽略。

With this preparation, this dissertation tries to probe the process of how the expostulation tradition of Chinese poetry came into being.

这个传统并不是从一开始的《诗经》、楚辞时代就已经定型,而是经过了汉代人的理论改造,最终得。。。

Everybody is whooping, time fast passing, Huang Qu senior official yellow Jinlong is wanting anxiously measured this kind of steam corrosiveness actually how, actually can'tfind the material in the bare planetoid surface which any may test, the returns control center, collects each material to come back to be able to waste the massive precious time, therefore then without hesitation stretches out own left hand,"thick outer space clothing glove which wears with the left hand, may probe the outer space exposure suit whether can resist this kind of unknown corrosion, if does not have the question, we might attack boldly!"

正在大家议论纷纷的时候,时间正在快速的流逝,黄区长官黄金龙急切地想测测这种蒸汽的腐蚀性究竟如何,在光秃秃的小行星表面却找不到任何可以测试的材料,返回指挥中心,收集各种材料回来就会浪费大量宝贵的时间,于是便毫不犹豫地伸出自己的左手,"用左手戴着的厚实太空服手套,就可以试探一下太空防护服是否能够抵御这种未知的腐蚀,如果没有问题,我们就可以大胆进攻了!"

Should not a magistrate be not merely the best administrator of the law , but the most crafty expounder of the chicanery of his profession , a steel probe to search hearts , a touchstone to try the gold which in each soul is mingled with more or less of alloy ?

一个法官除了无尽职守地按法律行事以外,除了极技巧地解释他工作上耍的诡计之外,难道不该做一枚可以探测心脏的钢针,一块可以测验出灵魂中含有多少杂质的试金石吗?

On the other hand, the longer he stayed in the US, the more he is inclined to Chinese traditional culture. He drew upon Chinese calligraphy, a language that integrates both abstraction and expressivity, attempting to probe the melting of western and Chinese art in the contemporary context.

另一方面,他在美国生活的时间越长,他愈发怀想中国传统文化的价值,其中特别思考和借鉴了中国书法这种融抽象性和表现性为一体的艺术语言,从而旨在探索中西艺术在当代层面上的融合。

In order to probe into the anti-friction mechanism and effectiveness of turbulent zone of flexible pipe ,extensometer with high accuracy and high-frequency response characteristics is used to study the frequency variation feature of flexible pipe s outer diameter with Reynolds number.

为探讨柔性管的紊流区域减阻效果的产生机理,采用高精度高频率响应特性的应变仪对柔性管外径随雷诺数的频率变化特性进行了研究。

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