查询词典 primer

Glass-epoxy system used for steel parts, casting a variety of mechanical parts or electronic components as a sealing material, the preparation of polyamide anti-corrosion paintings, as well as a zinc-rich epoxy primer, epoxy primer Iron Cloud, epoxy stone painting, epoxy paint, bituminous coal, chemical corrosion, paint, etc. have also been used widely

用于制环氧玻璃钢制件，浇铸各种机械零件或作为电子元件的密封材料，配制聚酰胺防腐涂料，以及用作环氧富锌底漆、环氧云铁底漆、环氧石漆、环氧煤沥青漆等，在化工防腐，涂料等方面也有广泛用途。

The second reaction mixture consisted of 5uL 10×PCR buffer,0.5uL 20mmol/L dNTP,1uL of primer R2(25umol/L),0.5uL Taq(2.5U),1.5uL product of the first PeR reaction, 43μL of double-distilled water,the whole system was 50μL;The reaction was run under the following conditions:DNA pre-denaturation at 94℃for 5 min;DNA denaturation at 94℃for 30 s,primer annealing at 55.6℃for 1min,extension at 72℃for 30s,for 25 cycles, and extension at 72℃for 5min.

循环的条件为：94℃变性5min，从94℃变性30s、58.8℃退火1 min到72℃延伸30s共25个循环，最后72℃延伸5 min。

The first reaction mixture consisted of 5uL 10×PCR buffer,0.5uL 20mmol/L dNTP,1uL of primer F1、R2(25umol/L),0.5uL Taq (2.5U),FTA filtering,42μL of double-distilled water,the whole system was 50μL;The reaction was run under the following conditions:DNA pre-denaturation at 94℃for 5 min;DNA denaturation at 94℃for 30 s,primer annealing at 58.8℃for 1min, extension at 72℃for 30s,for 25 cycles,and extension at 72℃for 5min.

第一轮适宜反应体系分别为：5uL的10×PCR buffer，0.5uL的20mmol/LdNTP，引物F1、R2各1uL(25umol/L)，0.5uL的Taq酶(2.5U)，含有基因组DNA模板的滤膜，用去离子水补足到50 uL。

Through comparing exon sequences at both ends of introns of CHD genes of jungle fowls and cygnets, exons on two ends thereof are respectively designed with an upstream primer and a downstream primer: Psf: 5'CAAGGATGAGAAACTGTGCAA3'; and Psr: 5'GAATATCTTCTGCTCCTACTG3'; and the Psf/Psr primers are used for amplifying the CHD genes on Z and W chromosomes of meat pigeon feather pulp genome DNA, and finally the sex is identified according to the band type through 10 percent polyacrylamide gel electrophoresis and silver staining, wherein, female shows two bands and male shows one band.

通过原鸡和小天鹅CHD基因内含子两端的外显子序列比对，在两端外显子上分别设计上下游引物：Psf：5'CAAGGATGAGAAACTGTGCAA3'；Psr：5'GAATATCTTCTGCTCCTACTG3'；再以Psf/Psr引物扩增肉鸽羽髓基因组DNA的Z和W染色体上的CHD基因，最后经过10％聚丙烯酰胺凝胶电泳、银染，根据带型判断性别，其中雌性为两条带，雄性为一条带。

Detail Contents: Genetic disorders -- Immune deficiencies -- Breast cancer -- Colon cancer -- Melanoma -- Cystic fibrosis -- Hemophilia -- Liver disease -- Cardiovascular disease -- Muscular dystrophy -- Alzheimer's disease -- Parkinson's disease -- Huntington's disease -- Viruses: the cornerstone of gene therapy -- Viruses are living crystals -- Viral genomes may be RNA or DNA -- Viruses evolved from plasmids -- Viruses know how to infect cells -- The virus as a gene vehicle -- Viruses used in gene therapy -- Ashi DeSilva: a promising start -- Clinical trials defined -- Cells of the immune system -- Adenosine deaminase -- Preliminary research -- Clinical procedure for ADA gene therapy -- The DeSilva clinical trial -- Jesse Gelsinger: down to earth -- Ornithine transcarbamylase -- Preliminary research -- Clinical procedure for OTC gene therapy -- The Gelsinger clinical trial -- The investigation -- Concluding remarks -- Future prospects -- Safer vehicles -- Reducing immune rejection of the vector -- Improved risk assessment -- Redesigning human anatomy and physiology -- Ethics of gene therapy -- The Belmont report -- Clinical trials -- Physiological enhancement -- Cosmetic applications -- Legal issues -- Regulatory agencies -- The Gelsinger legal trial -- International regulation -- Resource center -- Eucaryote cell primer -- Recombinant DNA primer -- The human genome project -- X-linked severe combined immunodeficiency (SCID-X1)-- Alzheimer's disease -- Huntington's disease.

细节内容︰遗传疾病-免疫的缺乏-乳腺癌-结肠癌-黑瘤-囊性纤维变性-血友症-肝疾病-心血管疾病-肌营养不良-早老性痴呆病-帕金森疾病-亨廷顿疾病-病毒︰基础的基因治疗-病毒在活著水晶--病毒的基因可能是RNA或者DNA --病毒从plasmids被逐步形成--病毒知道怎样感染细胞--作为一辆基因车辆的病毒--基因治疗使用的病毒-Ashi DeSilva︰有希望开始-临床试验确定--细胞的这免疫系统-Adenosine deaminase-初步研究-临床程式给埃达基因治疗--这DeSilva临床试验-婕西Gelsinger︰到地球-Ornithine transcarbamylase-初步研究-临床程式给OTC基因治疗-- Gelsinger临床试验-调查-达成评论-前景-更安全的车辆--矢量的降低免疫的拒绝-改进风险估计-重新设计人解剖学和生理学--伦理学的基因治疗-那些贝拉蒙特报告-临床试验-生理提升-美容应用-法律问题-协调机构-- Gelsinger 合法审讯-国际管理-资源中心人物-Eucaryote信元第一-Recombinant DNA 入门--人类基因工程-- X 连结的严重的结合的免疫缺陷(SCID-X1)-早老性痴呆病--亨廷顿的疾病。

Cleanup drastically rust on paint area which is damaged of armor plate and other steel components through machine way,lapped finishing lightly around area on paint floor and remove oil dirt, then wipe anti-corrosive primer immediately and wipe top-coat when the primer is dry.

Oslash；用机械方法彻底清除钢板和其他钢质部件上的油漆损伤区域的铁锈，轻轻研磨油漆层周围并除去油污，然后立即涂上防锈底漆，待底漆干后涂上面漆。

The sensitivity of the stdA-specific primer set was demonstrated on a Salmonella-free swab sample from a pork carcass surface, which was then artificially contaminated with different concentrations of S. Typhimurium. Inoculation studies showed that stdA-specific primer set detected Salmonella in samples having initial 4.4 × 100 CFU/ml of Salmonella inoculum prior to the enrichment step in the presence of 4 × 107 CFU/ml of non-Salmonella florae.

并且进行接种试验，屠体液中非沙门氏菌细菌数量为4× 107 CFU/ml，当4.4 × 100 CFU/ml的沙门氏菌加上屠体液於培养液中增殖后，可以使用stdA成对引子藉由PCR方式看到stdA的产物，表示stdA成对引子在PCR反应液中存在沙门氏菌及其他菌种时，仍然可以顺利进行反应，而不会受到影响。

Experimental results of G-type primer in a 100ml closed bomb show that this system is reliable and can quantitatively reflect primer ignition capability.

经对 G 型底火在 100 ml 爆发器内的试验结果表明，该系统测试方法可靠、结果稳定、能够定量反映底火点火能力状态。

Specific bands of D genomes and AB genome in four diploids, four tetraploids and four hexaploids, respectively, could be also amplified by means of M70/E5 1 and M711E5 1 primer combinations screened from twenty pairs of AFLP primer combination for test.

对二倍体、四倍体和六倍体小麦各4个材料的DNA进行AFLP扩增，从20对引物组合中筛选出M70/E51和M71/E51两个引物组合能扩增出D染色体组或AB染色体组的特异性带。

General Paint external walls of scrims standard closed Primer is set high alkali, high adhesion, water resistance and anti-salting-out that combines the functions of the external walls of water-borne closed primer.

漆将军标准型外墙抗碱封闭底漆是集高度抗碱、高附着力、防盐析及耐水功能为一体的外墙水性封闭底漆。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。