查询词典 primer

A first embodiment comprises a first common primer sequence, a first template homology region, a cleavable site, a second common primer sequence and a second template homology region.

第一个实施方案包含第一通用引物序列、第一模板同源性区、可切割位点、第二通用引物序列和第二模板同源性区。

By using SRAP technology, a polymorphism primer of E2M5 was successfully selected. The primer E2M5 can be used to test hybrid purity of a small fruited diploid watermelon variety Hongxiaoyu.

采用SRAP技术，成功筛选出1对多态性好的引物对E2M5，对二倍体小果型西瓜品种红小玉杂交种纯度开展了分子鉴定研究。

These primer combinations produced a total of 1 119 bands, 229 specific loci (including 54 absent bands, and 175 monomorphic bands). Among them, 983 polymorphic bands, accounting for 88%, were detected. The percentage of identification per primer combination was as high as 100%. The average PPB of 14 foreign cultivars was 35.86% and the average PPB of seven domestic cultivars was 31.51%.

结果表明：8对引物共产生1 117条谱带， 229个特异位点（其中缺失带54条、单态带175条），多态带983条，多态带的比例为88%；每对引物鉴别效率为100%。14个国外银杏观赏品种平均多态带的比例35.86%，7个国内品种平均多态带的比例31.51%。21个观赏品种之间相似系数为0.4899～0.8499。

The result showed that: 148 poly morphic hands were detected by use of, 22 RAPD primer and 158 allele were detected by use of 34 SSR primer.

利用筛选出的22条RAPD引物，检测到了148条有多态性的带；利用筛选出的34对SSR引物，检测到158个等位基因。

Rapid amplification of cDNA end:Retroverse transcription product of total RNA extracted from normal porcine tissue was used as the template,gene specific primers were designed and advantage 2 polymerase mix was used in PCR,of which using porcine genomic DNA as the template:forward primer was designed according to the acquired consensus region of human and pig FGL2 3′ sequences while reverse primer was designed from human FGL2 3′ end downstream sequence;TA cloning.

以猪正常小肠及心脏组织提取新鲜总RNA，反转录后作为模板，设计基因特异性引物，采用Advantage 2 聚合酶混合物进行PCR扩增；依据猪与人FGL2基因3′端已知同源序列设计PCR上游引物，以人FGL2基因3′末端序列设计下游引物，以猪基因组DNA为模板采用Advantage 2 聚合酶混合物进行PCR反应；PCR载体重组质粒DNA亚克隆扩增。

Objective With the help of Primer Premier 5.0 to design PCR primer about simulium COI gene and verify the efficiency and speciality of PCR about these primers.

目的 借助专有软件设计蚋科COI基因的PCR引物，并且检验所设计引物的PCR扩增效率和特异性。

Design one primer pairs for EphB2 receptor and one primer pairs for GAPD as internal control. The RT-PCR was used to analyze the expression of EphB2 receptor tyrosine kinase in gastric cancer, colon cancer, lung cancer, liver cancer, kidney cancer, spleen cancer and abdominal wall cancer. The results were analyzed using Bio-RAD UV transilluminator with Quantity One software system.

采用RT-PCR方法，分别检测胃癌、结肠癌、肺癌、肝癌、肾癌、脾癌、腹壁癌7种肿瘤组织及相应邻近正常组织中EphB2受体的表达，应用Bio-RAD凝胶成像系统及在Quantity One软件辅助下分析测定表达结果。

The ISSR fingerprinting of 18 Japanese plum cultivars and 12 plum species or variety were established by using optimum system with primer 5"-9T-3"and 5"-9C-3", respectively. The identification rate of plum cultivars and species or variety was 100%. The effects of five important components on reaction of SSR were studied with Prunus salicina cv. Meilili. The results showed that the optimum concentration of five important components i.e. Tag DNA polymerase, Mg +, single primer, template DNA and dNTPs in 25 μL reaction system of SSR were 1.5u, 2.0mM, 0.8 μ M, 30~40ng and 0.16~0.24mM, respectively. The dendrograms of these cultivars was contructed according the Jaccard coefficient of similarity.

利用该优化体系，以5′-_9T-3′为引物，构建了中国李18个品种的ISSR指纹图谱，该引物可将这些品种完全区别开来；以5′-_9C-3′为引物，构建了李属6类种质资源的ISSR指纹图谱，该引物区分率为100％。4、中国李品种适宜的SSR反应分析体系是：25μL SSR反应体系中，Taq DNA聚合酶、Mg~(2+)、每个引物、模板DNA和dNTPs等5种成分的适宜浓度分别是：1.5u、2.0mmol·L~(-1)、0.8μmol·L~(-1)、30～40ng和0.16～0.24mmol·L~(-1)。5、采用RAPD、ISSR和SSR等三种分子标记技术对中国李品种资源54份和2个欧洲李品种进行了遗传多样性分析，并以品种间的遗传相似系数构建了Jaccard聚类树状图。

Genetic diversity of 105 Chinese key cottons with Fusarium and Verticillium wilts resistance was studied based on AFLP markers. Twenty primer combinations were selected to perform the AFLP fingerprinting from 100 primer combinations.

利用AFLP分子标记技术，对我国20世纪50年代开展抗枯、黄萎病育种以来培育的105份骨干品种的遗传多样性进行了研究。

For example: zinc-rich epoxy primer, epoxy primer Iron Cloud, epoxy stone painting, epoxy coal tar pitch paint etc,.

环氧富锌底漆、环氧云铁底漆、环氧石漆、环氧煤沥青漆等。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。