查询词典 primer

A 1172 bp fragment was detected for Wx-7A gene using dominant marker primer from the Wx-A1 normal wheat, a 593 bp and a 574 bp fragment were detected using codominant marker primer from the Wx-A1α normal and Wx-A1 null wheat, respectively.

STS标记结果中，Wx-7A位点的显性标记引物在野生型中扩增出一条1172bp的特异带，即Wx-A1α基因出现；Wx-7A位点的共显性标记引物在野生型中扩增出一条593bp的特异带，在缺失类型中扩增出一条574bp的特异带。

The products amplified by primer 2 had polymorphism. The sequencing results indicated that there were two nucleotide mutations( C→A and C→T )at exon 1183bp and 336bp of hircine GDF9 gene, but the two mutations did not cause amino acid change. The products amplified by primer 3 had polymorphism.

所设计的6对引物扩增产物中共有3对发现了多态，引物1经SSCP分析虽然没有发现多态但经克隆测序却在外显子1编码区前-4bp处检测到A→C的突变。

Sex of early embryos was determined by PCR using double primers (Y-specific DNA primer BOV-97,157 bp; Bovine α-lactalbumin primer in male and female,109 bp).

利用双引物PCR技术对优质良种牛体外受精的冷冻胚胎进行性别鉴定，并对分割后的半胚分别进行PCR扩增和染色体分析，两种方法的判定结果相吻合。

Based on the principal of PCR-primer design, the usage of two kinds of popular primer-design software was reviewed detailedly, including their merit, demerit and usage skills.

在PCR引物设计原则的基础上，详细介绍了两种常用引物设计软件的基本使用方法，并对其各自的优缺点进行了比较。

A band of 850 bp was generated in the line of non-parthenocarpy by the primer N92 both in the parent and in the DNA pool. Accordingly, it is possible that the primer N92 might be used to phenotyping the trait of cucumber parthenocarpy, and further we calculated the genetic distance between the marker and cucumber parthenocarpy trait that might be 18.9 cM.

用65条ISSR引物进行PCR扩增筛选，其中引物N92在亲本和DNA池之间扩增出一条大小为850bp多态性片段，经F2代群体135个单株验证，该片段稳定出现，在单性结实植株中表现为无带，在非单性结实植株中表现为有带，可以作为鉴别单性结实与非单性结实的标记引物。

Last, I sealed the primer with a primer sealer.

最后，我封锁了引物，以物密封。

Upon initiation of PCR after the denaturation phase, the single-stranded DNA fragments flanked by inverted terminal repeats may form either self-annealing "pan-like" structures (preventing the primer binding to its complementary binding sites and suppressing the PCR) or DNA/primer hybrid structures.

经聚合酶链反应开始后，变性阶段，单链 DNA片段两侧末端反向重复序列可能两种自我退火"泛，如"结构（防止引物结合到其互补性结合位点和制止的PCR ）或DNA /引物混合结构。

AFLP marker technique was used to investigate the genetic relationships between 50 accessions of maize and its wild relatives including teosinte, Tripsacum and Coix With twenty one AFLP primer combinations of PstⅠ/MseⅠ, 641 AFLP amplified bands were obtained, including 586 polymorphic bands with an average of 30 52 per primer combination and the polymorphism percentage of 91 4% Cluster analysis using UPGMA method based on the data of AFLP showed that the 50 materials could be divided into...

用AFLP分子标记技术对 5 0个有代表性的玉米及其野生近缘种大刍草、摩擦禾、薏苡材料进行了遗传关系分析。利用 2 1个PstⅠ MseⅠ引物组合检出可辨认带共 6 4 1条，其中多态性带 5 86条，平均每对引物得到 30 5 2条多态性带，多态性 91 4 %。聚类分析结果表明，可以将 5 0个材料分为 3大类，与已有的玉米族分类一致，但与玉蜀黍属内的种间和种内分类存在差异，说明大刍草各种内存在较大的遗传多样性。本实验发现玉蜀黍属与摩擦禾关系比与薏苡的关系更近，但并没有发现玉米与小颖玉米亚种比与其他大刍草的遗传关系更近。

Shop primer-coated steel: coating damage, corrosion and the white rust on zinc primer that require secondary anticorrosive , burnish to St3 level.

涂有车间底漆的钢材：漆膜损伤处、锈蚀处及含锌底漆上的白锈，须进行二次除锈，打磨至 St3级。

80 Individuals of ATA-A F2 plants were used for RAPD molecular mapping of the monoecious gene by bulk segregant analysis. 840 random primers were chosen for polymerase chain reaction amplification between monoecious pool and andromonoecious pool, the results showed that: primer S128 was tightly linked to the monoecious character, and primer S493 was tightly linked to the andromonoecious character.

以甜瓜单性花系ATA-A系与两性花品种YJX-1的杂交F2代的80个单株为材料，利用群分法进行单性花基因有关RAPD分子标记研究，筛选分析了840个10bp随机引物的PCR扩增谱带，结果表明，随机引物S128在1180bp，S493在930bp处产生与性别相关的特异性条带，S128的特异条带与单性花性状相关，S493的特异条带与两性花性状相关。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。