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To investigate the mechanism of wood formation and lignin biosynthesis, it is important to understand the function and characteristic of OMT. In the present study, a set of primer was designed based on the conserved region of OMT genes from several woody and non-woody plants, to clone the counter part gene by PCR and RACE from Chamaecvparis formosensis Matsum., Chamaeeyparis obtusa var. Jormosana, Abies kawakarnii, Tsuga ehinensis var. Jormosana and Taiwania eryptonierioide Hayata. The cloned genes were sequenced and their amino acid sequences predicted.

我们自资料库中收集并分析已发表之草本和木本植物caffeoyl CoA3-O-methyltransferase序列，在具高保守性的序列中设计了一对引子，并对红桧、台湾扁柏、台湾冷杉、台湾铁杉和台湾杉进行OMT基因的钓取，同时配合RACE的策略，成功获得红桧、台湾扁柏、台湾杉的全长基因及台湾冷杉与台湾铁杉之部分基因片段。

The high-tech companies abroad, development and production of polyester resin E-glass primer twistless Stubbs molding insulation material products, is a new type of insulation structural material.

本公司采用国外高新技术，开发生产的聚酯树脂无碱无捻玻璃丝引拔成型绝缘型材制品，是一种新型绝缘结构材料。

The genomic DNA of Unio douglasiae, collected from Suya Lake, Henan Province, was used as template DNA to optimize the RAPD-PCR reaction conditions including the concentration of DNA template, random primer, dNTPs, Mg2+ and Taq DNA polymerase and the annealing temperature for thermal cycle.

以河南宿鸭湖圆顶珠蚌的基因组DNA为模板，对淡水蚌类RAPD反应体系中的模板DNA浓度、随机引物浓度、dNTPs浓度、Mg~(2+)浓度、Taq DNA聚合酶浓度和和热循环参数中的退火温度进行了优化。

The antiablation of silicone rubber liner can be improved both by changing the structure of unvulcanized rubber and adding antiablative fillers,and the bond strength between propellant charge and the liner would be increased largely by coating the primer liquid on the charg...

硅橡胶包覆层中填料的粒径对硅橡胶的补强效果十分重要；可从改变生胶结构、添加耐烧蚀填料两方面来改善硅橡胶包覆层的耐烧蚀性；在推进剂药柱表面上涂覆底涂液可以大幅提高硅橡胶包覆层与推进剂的粘接强度。

And MSAP with 24 primer was used for analysis in Yesso scallops with orange/white muscle, it was showed that there were 46 loci which had differences in the degree of methylation between the population with orange muscle and the white, among of which 18 were methylated or semi-methlated loci, which may be related to the appearance of orange muscle.

采用24对引物组合对橘红/白色闭壳肌虾夷扇贝群体进行了MSAP分析，结果表明，甲基化程度存在差异的位点46个，其中18个位点为甲基化或半甲基化位点，推测橘红色闭壳肌的发生可能与这些位点有关，它们调控了该性状的表达。

According to CAV Fip genes sequence,4 paris of primer were designed to amplify the Fip genes with PCR method.Complete Fip gene sequences of all CAV strains were shown to be consisted of 1 629nt and 1 631nt and encoded 542 an d 543 amino acids from ATG start coden.

根据已发表的CAV纤突基因序列，用PCR方法，对4个CAV－2毒株和4个CAV－1毒株的纤突基因进行了扩增和测序，测定的核苷酸序列经推导得到分别编码543和542个氨基酸的CAV纤突蛋白全序列。

In this study,one pair of specific primer for mature chicken interleukin-18(ChIL-18) cDNA was designed and synthesized according to the previously published gene sequence of ChIL-18.The full length cDNA gene of ChIL-18 encoding mature active protein was amplified from LPS–stimulated MDCC-MSB1 cells by Reverse Transcription-Polymerase Chain Reaction.Then it was cloned into pMD18-T vector. Sequencing analysis showed that the nucleotide sequence of this ChIL-18 mature protein gene was 5l0bp including the stop coden and the same as the published ChIL-18 cDNA sequence by Schneider K.

本研究根据已发表的ChIL-18成熟蛋白(mature ChIL-18，mChIL-18)的cDNA基因序列，设计一对特异性引物，应用反转录-聚合酶链式反应(reverse transcription-polymerase chain reaction，RT-PCR)技术从脂多糖刺激10小时活化的马立克氏病成淋巴细胞样细胞系MDCC-MSB1的cDNA中扩增出编码鸡IL-18成熟活性蛋白的全长基因，对扩增片段进行T-A克隆，经PCR、酶切鉴定及测序验证，成功获得ChIL-18成熟蛋白完整基因的克隆。

In this paper, RAPD, ISSR, and SSR reaction system of the fruit tree were established, and the genetic diversity of 56 genotypes of Japanese plum were analyzed using RAPD, ISSR, and SSR markers. The optimal reaction of RAPD was studied with 5"-GACCGCTTGT-3" primer for cv. Fenghuali.

本文选择了56个中国李品种资源(包括2个欧洲李品种)，从叶片基因组DNA提取方法入手，在建立和优化中国李RAPD、ISSR和SSR反应分析体系的基础上，利用这三种分子标记对中国李品种资源进行聚类分析，旨在促进中国李品种资源分类和遗传育种研究。

The results showed that the optimum concentration of five important components i.e. Tag DNA polymerase, Mg2+, primer, template DNA and dNTPs in 25μL RAPD reaction system were 1.0u, 2.5mM, 20ng, 40ng and 0.2mM, respectively. The RAPD fingerprinting of five Japanese plum cultivars were established by using optimum reaction system with ten random primers.

结果表明：1、改良陈大明法是提取中国李叶片基因组DNA的适宜方法。2、以5′-GACCGCTTGT-3′为随机引物，建立了优化的中国李RAPD反应分析体系：25μL反应体系中，Taq酶、Mg~(2+)、引物、模板DNA和dNTPs 5种主要成分的适宜浓度或用量分别是：1.0u、2.5mmol·L~(-1)、20ng、40ng和0.2mmol·L~(-1)。

The best reaction system for ISSR in our research followed as: the whole volume of the reaction is 20μL, and the mixture in this reaction included 50ng DNA, 2.0mmol/L MgCl_2, 0.2μmol/L primer, 200μmol/LdNTPs, 0.5U TaqDNA polymerise and 1×PCR buffer; and the PCR program was 94℃7min, 1 cycle; 94 ℃ 30s, specific temperature 45s, 72℃ 90s, 38 cycles; 72℃ 10min, 1cycle.

对两种标记的检测效果表明，ISSR和RAPD标记所得结果呈极显著相关，证明这两种标记技术均可用于樟科植物的遗传多样性和亲缘关系研究，但ISSR效果明显优于RAPD。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。