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In this study, SSR analysis was performed on five Secale species, four Triticum species, and a Triticale with branch-like using 102 pairs of microsatellite primers. A 387bp specific DNA fragment FZ387 (Accession No. EF179137), was obtained from Triticale with branched-like by primer Xgwm614; however no amplification result revealed in Secale.

利用102对微卫星引物对5份黑麦、4份普通小麦和1份分枝小黑麦进行SSR分析，引物Xgwm614能在分枝小黑麦中扩增出一个387bp的特异DNA片段（记为FZ387，GenBank登录号为EF179137），而黑麦未能扩增出。

Selecting water-borne styrene/acrylic anti-rust latex of soapless polymerization technology as binder,iron oxide red and aluminum triphosphate as anti-rust system and nano SiO_2 paste,we prepared water-borne nano-modified styrene/acrylic anti-rust primer,properties of which reached the level of solvent-borne alkyd antirust coatings.

本试验选用无皂聚合工艺制备的水性苯丙防锈乳液为基料，氧化铁红与三聚磷酸铝组成防锈体系，添加纳米SiO_2浆料，制备的水性纳米改性苯丙防锈底漆性能达到溶剂型醇酸防锈涂料的水平，并分析了乳液、防锈颜料、纳米SiO_2对水性苯丙防锈底漆性能的影响。

Before being painted, the painting plates are made thirteen professional processes, such as cutting, stapling, Pasting, sawing, planing, skin texture, speckling, primer spraying.

本店油画所用板材，在正式画油画前，需经过切割、订、粘、锯、刨、打叽哩、点缀、喷底漆等13道专业工序，除去繁琐的人工成本外，就板本身的材料成本，也在油画布所做油画的10倍以上。

Markinf primer need good, souvenirs…-subterrene ＃fhand excellent adhesion, and our ink.

热熔底涂需有良好的瞬间热熔效果及优秀的附着力，与油墨层配合良好。

Synthesizc 2-methyl-5-acetamino benzene sulfochloride using p-toluidine primer reactant by aceylation and chlorosulfonation.

以对甲基苯胺为起始原料，通过乙酰化和氯磺化反应合成2-甲基－5-乙酰氨基苯磺酰氯。

The genomic DNA of 31 accessions of genus Tagetes was amplified with 11 primers, the bands produced by each primer ranged from 5 to 11, and 6.8 bands were obtained on average, the polymorphic loci ranged from 40%-100%.

用11个引物对31份万寿菊属材料进行PCR扩增，每个引物扩增的ISSR条带数在5-11条，平均每条引物能扩增出6.8条带，多态位点百分率为40%-100%。

To detection of Tilletia controversa Kühn sensitively and accurately, real-time PCR systems were developed. The species-specific primer pair CQUTCK4/CQUTCK5 and probe CQUP1 were designed based on a selected specific fragment (1322 bp) specific for TCK, and the SYBR Green I and TaqMan quantitative PCR detection systems were established with optimized reaction conditions.

建立荧光定量PCR体系以准确灵敏的鉴定小麦黑穗菌(Tilletia controversa Kühn， TCK)根据筛选的TCK独有差异基因片段(1322bp)设计特异性引物对CQUTCK4/CQUTCK5和TaqMan探针CQUP1，建立SYBR GreenⅠ荧光染料法和Taq Man水解探针法定量PCR检测体系，并对体系进行优化。

According to pastuerella toxA gene rank, a pair of primer was designed and synthesized. The rank of toxA was amplified by pastuerella toxA in clinical.

根据巴氏杆菌毒力基因toxA序列设计合成1对引物，以临床分离的虎源巴氏杆菌为模板，通过PCR技术，扩增出toxA基因片段。

With the primer P1, the amplified fragments from the 2 trematodes were quite different, while with P2, 2 similar bands were found with 400 bp and 100 bp respectively.

斯氏肺吸虫和华支睾吸虫的P1扩增片段均不相同，而P2引物对两吸虫的扩增产物有2条相同的片段，分别为400、100 bp。

Microsatellite loci sequences of family Trichogrammatidae parasitoid wasp from Genbank were searched, and four microsatellite primer pairs that adapt to T.dendrolimi SSR analysis were screened.The PCR reaction conditions of microsatellite primers for T.dendrolimi were tested , and SSR reaction system of polymophic test for T.dendrolimi population genetic structure was established.The SSR molecular marker create new method for genus Trichogramma parasitoid wasp study.

在搜索Genbank中主要赤眼蜂属寄生蜂及与赤眼蜂亲缘关系相近寄生蜂微卫星座位序列信息的基础上，筛选了可以用于松毛虫赤眼蜂SSR分析的4对微卫星引物，摸索PCR反应条件，建立松毛虫赤眼蜂种群群体遗传结构多态性检测的SSR指纹标记体系，SSR分子标记技术为赤眼蜂属寄生蜂研究提供了新方法。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。