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Oleoresinous zinc chromate primer for steel ship.

钢船用油性船舶锌铬黄底漆。

The CMS line 9704A, the restorer 8001 and F, population of 9704AX8001 containing 120 individual plants were used for molecular mapping of the restorer gene by bulked segregant analysis. 515 decamer primers with arbitrary sequences were chosen for polymerase chain reaction amplification. It is found that the polymorphism between the parents could be detected by 43. 8% of the primer and one RAPD marker OPL09-76I was tightly linked to the restorer gene of the restorer 8001 with the overcross value of 4.17% and genetic distance of 4. 18cM. The marker was repeatable and easy to score.

以辣椒CHS系9704A、恢复系8001及其组合9704A×8001F_2群体120株植株为材料，利用群分法法进行恢复基因定位，通过515个随机引物的PCR扩增分析，发现43.8％的引物在双亲间表现出多态性，找到一个与辣椒CMS恢复系8001中的恢复基因紧密连锁的分子标记OPL09_(-763)，该标记与8001的恢复基因的交换值为4.17％，遗传距离为4.18cM。

The invention relates to a PP-based composite composition which is directly paintable without treatment of a primer comprised of chlorinated polyolefins.

本发明涉及到一种PP基复合组成，它可以直接涂装而无须氯化聚烯烃底漆处理。

Pcr products were visualized under uv after agarose electrophresis. results: the hypermethylation of blu gene promoter was detected in all the 12 frozen tissue cases (100%) and 8 out of 35 paraffine embedded tissue cases (23%) by methylated primer and the hypermethylation of arf was observed in 1 of 20 frozen cases and 3 of 35 paraffine embedded cases.

结果：在12例鼻nk/t细胞淋巴瘤冰冻组织病例中，12例均有blu基因高度甲基化，仅1例有arf基因高度甲基化；35例石蜡组织病例中，blu基因高度甲基化8例，arf基因高度甲基化3例。

It is designedthat a pair of primers are used in 3"-RACE, two pairs of nesting primers and one 5"phosphated primer are used in 5-RACE, eight pairs of general primers are used inPCR which cover all the genome of these two isolated rabies viruses.

根据已测定的狂犬病毒株全基因组序列，用分子生物学软件设计了用于3'-RACE的接头引物一对，用于5'-RACE的2对嵌套引物、1条5'磷酸化引物，用于普通PCR的8对引物，这些引物扩增序列覆盖了整个病毒全基因组序列，具有一定的重叠区域。

In this study, used Multiplex-PCR method to detection AAC of AAC_ITS and AAC_rpoB, used Multiplex-PCR method to detection BG of BG_ITS、BG_gyrB and BG_rpoD, used Multiplex-PCR method to detection PCH of PEC_ITS, idgA and idgB, used Multiplex-PCR method to detection AAC、BG and PCH of AAC_ITS、BG_ITS、PEC_ITS and idgA. The result reveals which were designed primer of specific can detect the existence of different phytopathogenic bacteria. These technique will use to develop the standard operation procedure of phytopathogenic bacteria disease in orchids.

在多引子组之研究上，AAC_ITS及AAC_rpoB之组合可用於褐斑病菌之检测；BG_ITS、BG_gyrB及BG_rpoD之组合可用於叶斑病菌之检测； PEC_ITS、idgA及idgB之组合可用於软腐病菌之检测；AAC_ITS、BG_ITS、PEC_ITS和idgA之组合则可用於三种兰花细菌性病原之检测，分析结果显示所使用之引子组可以快速、灵敏且专一性的检测细菌性褐斑病、细菌性叶斑病及细菌性软腐病，并且缩短检测时间，未来将可以应用於兰花细菌病害之监测。

I have made the same helmet but better from 18 guage steel, it took about 35 hours but i finished it well, it is painted with 3 coats of platework red oxide primer and finshed with the high gloss red and gold and finished with automotive clear coat.

我已经说了同样的头盔，但更好的18号钢，花了约35个小时，但我完成得很好，这与3的platework红色外套氧化底漆和finshed高绘有红色和金色光泽和成品汽车清漆。

The10 primer combinations were high polymorphic primers, which could be applied widely on Pyrus L.By AFLP technique.

所选出的引物组合共扩增出371个位点，其中多态性位点306个，占总位点数的82.5%，每个引物组合扩增的位点数变化在23-55个之间，平均37.1个。

Kasmira and cobia（Rachycentridae: Rachycentron canadum）were determined using long PCR and primer-walking methodology with universal primers, and their GenBank Accession numbers are FJ171339, FJ416614, FJ824741, FJ824742 and NC_011219, respectively.

malabaricus）和军曹鱼科的军曹鱼的线粒体DNA基因组全序列（GenBank序列号分别为FJ171339， FJ416614， FJ824741， FJ824742 和NC_011219），表明本文所用全序列测定体系的通用性较强，操作简单。

Methods: Total RNA was extracted from esophageal carcinoma tissue, the primer containing special enzyme was designed, NY-ESO-1 fragment was amplified by RT-PCR, and then was cloned into the expression vector pET-15b by LP Recco PCR Cloning. The recombinants plasmid pET-15b-NY-ESO-1 were identified by restriction endonucleases digest analysis and sequence analysis.

从人新鲜的食管癌组织中提取总RNA，通过RT-PCR技术扩增NY-ES0-1基因3'端的340bp片段，采用靶向克隆法将目的基因插入到原核表达载体pET-15b中，得到重组表达质粒pET-15b-NY-ESO-1，经过筛选，挑选出阳性克隆进行XhoⅠ和BamHⅠ双酶切图谱分析、PCR检测和扩增产物核苷酸序列分析等，鉴定所构建的原核表达载体。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。