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The resulting populations of proteins were considered as induced protein positive to heat stress. Using LC-ESI MS/MS, several proteins were identified, including glyoxalase I and 3beta-hydroxysteriod dehydrogenase. Degenerative primers were designed and RACE technique was applied to obtain the complete gene sequences of the two-mentioned proteins from heat-sensitive and heat-tolerant tomatoes.

经由此研究，成功地找出多个具有高度差异性的蛋白质，在功能分析及归纳整理之后，挑选了其中两个蛋白质glyoxalase I和3beta-hydroxysteriod dehydrogenase，设计degenerative primer，并利用RACE技术将上述2种蛋白质在二种番茄品系中的完整基因序列定出。

Third, growth differences between females and males were analyzed, and sexspecific DNA fragments were attempted to screen by 120 RAPD primer amplification in the gonochoristic offspring.

三是在分析银鲫雌雄个体生长差异的基础上，采用RAPD引物对同一繁育组合中的雌雄个体进行扩增，试图寻找性别特异片段。

Methods Tow biovars of U. urealyticum in gonococcal urethritis group, NGU group and control group was detected by a universal primer-PCR-capillary electrophoresis assay.

使用通用引物－PCR-毛细管电泳法对淋菌性尿道炎组，非淋菌性尿道炎组和对照组中的溶脲脲原体的2个生物群进行检测。

This motivated us to design genus-specific oligonucleotides primer pairs which can assist in rapid detection of species of the genus Gordonia by means of PCR-specific amplification.

筛选Gordonia分离株不但可开发新放线菌资源，并且了解此类放线菌之分布及在生态中的角色，此外研究Gordonia属内菌种之亲缘关系，有助於了解各菌种之演化地位，并提供土壤中可靠且快速之鉴定结果。

The Gordonia specific 16S rDNA fragment (829 bp or 436 bp) was successfully amplified for all the reference Gordonia species with the designed primer pair G268F/G1096R or G699F/G1134R whereas, no amplification was noted for closely-related species from other genera.

研究结果显示，利用G268F/G1096R及G699F/G1134R两组Gordonia菌属特异性引子，可有效区别Gordonia菌属与其他放线菌菌属，并且利用此侦测技术已从台湾分离出36株Gordonia分离株。

Methods The current conservative sequence primer and probe were designed according to the gene sequence of moderate and strong NDV in GeneBank. Fuorescence ration RT-PCR was done to the special TaqMan probe of moderate and strong NDV .The proliferation of NDV in chicken embryo was evaluated after taking Shuanghuang Shadu Tuishao Gule comparing with the control group.

根据GenBank登录的NDV中、强毒力株F基因序列，选择包含裂解位点的保守序列设计通用引物和针对中、强毒力NDV的特异性TaqMan探针进行实时荧光定量RT-PCR，评价双黄杀毒退烧颗粒干预后NDV强毒在鸡胚中和在机体内病毒的增殖情况，并与未干预的对照进行了比较。

A chimeric primer was designated in which segment A near 3' end is complementary to HB plus strand just before the DR2 region gap, and segment B near 5' end is consensus to part of the human immunodefficient irus genomic sequence, without homogenetic relationship to HB genome.

设计一嵌合引物，使之3'端的A片断与HB正链互补，并恰好位于DR2缺口之前；5'端的B片断与人类免疫缺陷病毒的基因序列具有同源性，与乙型肝炎病毒的基因组无同源性。

Based on the degenerated primer, the cDNA of open reading frame of growth horme gene for black carp and Japanese crucian carp were cloned and sequenced through RT-PCR, which were used for the"all-fish"transgene construction and preparation of polyclonal antibody against GH for black carp.

基于鲤科鱼类生长激素基因序列，通过RT-PCR技术，克隆了青鱼、日本白鲫生长激素基因编码区cDNA序列，以此来构建&全鱼&基因载体和研制生长激素多克隆抗体。

In total, 272 loci were amplified with 4 primer pairs, of which 269 were polymorphic and the percentage of polymorphic loci was 98.89%. The Shannon diversity index for S. intermedius, S.

结果表明， 4对引物共扩增得到272个位点，其中269个多态位点，总的多态位点比例为98.89%。3个群体的香农多样性指数分别为： 0.2331 ± 0.1273、0.2005 ± 0.1385和0.2625 ± 0.1067。

The multi-drug resistance rate of Salmonella and E.coli were 91.80% and 100%.According to the genes sequence published in Genbank ,we analysed the value of Tm ,content of G+C, oligonucleotide dimer by DNAStar and Primer 5.0 software, and according to the construction of I integron ,we designed 3 pairs of primers to amplify intIl gene ,variable segment ,3"-conserved segment for detectting Salmonella and E.coli"s I integron.As a result , aimed genes were amplified and sequenced;The detection rate of I integron of 61 Salmonella and 48 E.coli were 81.97% and 66.67%.

据文献报道及GenBank中注册的基因序列，运用DNAStar软件分析同源率及Primer5.0软件对Tm值、G+C含量、引物二聚体等进行分析，根据Ⅰ类整合子的结构设计了3对引物，对Ⅰ类整合子的intI1、可变区、3'保守端进行扩增，检测沙门氏菌和大肠杆菌Ⅰ类整合子；扩增出了相应基因片段并测序；61株沙门氏菌和48株大肠杆菌中分别有50株和32株菌检测出Ⅰ类整合子，Ⅰ类整合子的检测率分别为：81.97％和66.67％。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。