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The effect of Sco on expression of RyR mRNA level of TSMC from asthmatic guinea pigs The presence of RyR mRNA was tested by RT-PCR using primer sets based on the 3'codding regions of the RyR genes.

说明哮喘组豚鼠TSMC上RyR2 mRNA表达明显降低，与对照组比较有显著差异（P＜0.01）；Sco组豚鼠TSMC上RyR2mRNA表达情况较哮喘组增强（P＜0.05）。

To improve detection efficiency and result accuracy, we developed an improved multiplex PCR technique for specific detection of GMO.①The assay strategy involves multiplex PCR amplification of the target with several biotinylated primer pairs in one tube, followed by the hybridization of multiplex PCR products and membrane-bound oligonucleotide probes. The multiplex PCR/probe hybrid is detected colorimetrically using an alkaline phosphatase conjugate and a chromogenic substrate.

在多基因定性检测技术方面，本文在优化并确定了多基因PCR反应条件的基础上，采用了两种方法对多基因PCR产物进行鉴定：①采用共价结合方法将多个探针固定在Biodyne〓C膜上，将5'端标记生物素的多基因PCR产物与膜上的探针进行杂交及显色反应，首次建立了GMO的多基因PCR-膜相杂交检测方法，实现了一个反应体系中同时检测多种转基因成分的目标，提高了检测效率与准确率。

Firstly, the recombinant engineering technology related vaccine preparation was set up, including: 1. the gene knock-out based on linear DNA target molecular in vivo; 2. the selection and counterselection technology of exogenous gene knock in definite site on chromosome; 3. the Gap-repair clone technology in vivo; 4. the recombination technology of single-chain DNA and the overlapping primer; 5. Set up the new selection and counterselection technology of neo-E.

本研究首先建立与疫苗制备相关的重组工程技术包括：①基于线型DNA打靶分子的大肠杆菌体内基因剔除技术，②选择与反选择外源基因定位敲入染色体上特定位点技术，③Gap-repair体内克隆技术，④单链DNA重组技术和重叠引物介导的DNA重组技术，⑤建立&抗生素-酶切位点&选择反选择技术新技术。

Thirty-seven accessions of wild Dactylis glomerata L. were used in assessment of genetic diversity by means of amplified fragment length polymorphism. Nine primer pairs were selected from 64 preparations and then used to analyze the plant materials.

应用扩增片段长度多态性技术，从64个引物对中筛选出9个，分别对37份鸭茅种质进行扩增，共得到400条清晰条带。

The results indicated that 18 ISSR primers and 14 wheat SSR primer pairs were available to analysis genetic diversity of Elymus species.

植物研究中的可应用性，用33个ISSR引物和137对小麦SSR引物对该属12个物种进行了分析。

In order to compare the usefulness of ISSRs and SSRs in Elymus species, 33 ISSR primers and 137 wheat SSR primer pairs were employed to detect 12 species of Elymus in this study.

为了比较ISSR和SSR标记在披碱草属

The amplified fragment length polymorphism technique was used to assess the genomic DNA polymorphism of four species Emberiza (cioides, leucocephala, tristrami and spodocephala). Eleven pairs out of thirty-nine AFLP primer pairs produced reproducible polymorphic DNA amplification patterns.

利用扩增片断长度多态性技术分析了鹀属4种鹀（三道眉草鹀Emberiza cioides，白头鹀Emberiza leucocephala，白眉鹀Emberiza tristrami，灰头鹀Emberiza spodocephala）基因组DNA的多态性。

The amplified fragment length polymorphism technique was used to assess the genomic DNA polymorphism of four species Emberiza (cioides, leucocephala, tristrami and spodocephala)Eleven pairs out of thirtynine AFLP primer pairs produced reproducible polymorphic DNA amplification patterns.

利用扩增片断长度多态性技术分析了鹀属4种鹀(三道眉草鹀Emberi za cioides，白头鹀Emberiza leucocephala，白眉鹀Emberiza tristrami，灰头鹀Emberiza spodocephala)基因组DNA的多态性。

Five of fifty random primers amplified DNA polymorphism hands in RAPD analysis of seed-derived seedlings and of in vitro regenerated plantlets taken at random. One primer eyen amplified the difference between the regenerated plants. This suggested that there was some variation in DNA in plants of M. sativa regenerated in vitro.

随机选取实生苗和下胚轴愈伤组织再生苗进行RAPD分析，结果表明，所用的50个随机引物中有5个扩增出差异性条带，甚至再生植株之间也有1条引物扩增的条带有差异，这说明经组织培养所得到的再生植株与实生苗相比，在DNA水平上发生了一定程度变异，并且这种变异也存在于再生植株之间。

Thirty PCR primer pairs were designed and synthesized, and then screened by a sample panel of 12 random selected Yangtze finless porpoises.

选择其中30条序列设计PCR扩增引物，并用12个随机选择的长江江豚样品进行多态性筛选。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。