查询词典 primer

In searching for RAPD marker linked to the genes controlling brachytic stem, 260 RAPD primers were applied to screen four parents of three combinations and RIL. Polymorphic bands revealed by the primer S-506 exhibited the best repeatability among all primers.

利用4个亲本和1个重组自交家系筛选260个RAPD随机引物，其中有1个引物S-506扩增出的多态性条带有较好的重复性。

In the RAPD system of Bryaceae the optimal concentration of template DNA was 2ng/μl, primer 0.4μmol/L, dNTP 0.2mmol/L, Mg2+ 2mmol/L, Taq DNA polymerase 1.25U, total volum 25μl. Several DNA samples were selected to choose the compatible primers.

通过单因素和正交试验对影响RAPD的因素进行考查，建立了优化的RAPD反应体系：dNTP为0.2mmol/L，随机引物0.4μmol/L，Taq DNA聚合酶1.25U，Mg2+为2mmol/L，模板DNA为50ng，扩增体系为25μl。

Methods The primer design is referring to thearticleof Bulter. Over 120 samples provided by the laboratoryare detected to verify the concordant results between miniSTR andcommon STR. We construct the miniSTR multiplex system usingdomestic Taq DNA polymerase, and detect the results by ABI—310Genetic Analyzer. We have alsostudied the sensitivity, accuracy, species-specificity and different PCR outcome under each annealtemperature.

方法参照Bulter等对THO1、TPOX、CSF1PO三个基因座miniSTR的引物设计，选用本教研室提供的123例无血缘关系个体的血样，进行THO1、TPOX、CSF1PO三个基因座PCR-miniSTR银染检测，检测结果与商品化试剂盒的检测结果进行一致性检验；采用国产DNA聚合酶构建miniSTR荧光标记复合扩增体系，用美国ABI-310基因分析仪进行检测；对该荧光标记复合扩增体系的灵敏度、准确性、种属特异性、不同退火温度下复合扩增的效果、陈旧血痕DNA的检测等进行研究。

The result showed that we have established a ITS-PCR system most suitable for Buxus sinica var. parvifolia, which came out total volume 50μL reaction system, was as follows: 2.0mmol/L Mg(superscript 2+), 0.3μmol/L primer, 0.3mmol/L dNTP, 240 ng/50μL DNA template, 1.75 U/50μL TaqDNA polymerase and the anneal temperature with 56℃.This optimized ITS-PCR system might ensure the purity and quality of ITS-PCR production. In this study, the length of the ITS fragment of Buxus sinica var.

实验结果表明，在总体积50μL的反应体系中，建立了最佳ITS-PCR扩增条件：Mg(上标 2+)浓度2.0mmol/L、引物浓度0.3μmol/L、dNTP浓度0.3mmol/L、DNA模板浓度240ng/50μL、TaqDNA聚合酶的用量1.75U/50μL和退火温度56℃，该优化体系保证了珍珠黄杨ITS-PCR产物的纯度和质量要求。

In this research, we extracted the genomic DNA from the Buxus sinica var. parvifolia leaves by using the modified CTAB approach and then used as the template DNA. Base on the invariable quantity of TaqDNA, we att empted to establish and optimize ITS-PCR amplification system for Buxus sinica var. parvifolia by employing L9 (3^4) orthogonal design with four factors, i.e. template DNA, Mg(superscript 2+), dNTPs and primer.

本研究利用改良CTAB法从珍珠黄杨叶片中提取基因组DNA作为模板，在TaqDNA聚合酶量不变的基础上，利用正交设计L9(3^4)对4个因素（模板DNA、Mg(上标2+)、dNTP和引物）在3个水平上对珍珠黄杨ITS-PCR反应体系进行优化。

The purpose of this study was analysis and tracking the heredity stability of Alpaca through amplifying special fragment from mitochondrion genome. A pair of primer was designed according to those sequences of Camelidae animal submitted in GaneBank and a fragment of 1014 bp was cloned.

摘 要：本试验对GaneBank中登录的部分骆驼科动物线粒体12srRNA/tRNA-Val/16rRNA基因序列进行同源性比较，并借助DNAstar软件设计引物，扩增并进行序列分析，旨在揭示其遗传变异的规律。

In this study 24 cpSSR primer pairs were screened, and four pairs were found producing DNA fragment length polymorphism from the tested populations of Castanea mollissima.

对24对cpSSR通用引物进行筛选，选用4对呈现多态性的引物对板栗的4个野生居群和9个地方品种群进行遗传分析。

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The slices of pear leaf samples infected by apple chlorotic leaf spot virus were prepared for situ RT-PCR and were detected by primed in situ labeling technique using special primer.

选用带苹果褪绿叶斑病毒的香梨叶片，制备成适合进行原位RT-PCR的石蜡切片。

Eighteen SSR primer pairs were designed and synthesized.The factors which affected on the SSR reaction of Toona ciliata var.

利用所合成的引物优化SSR反应体系，对影响SSR反应的各个因子进行了探讨。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。