查询词典 primer

Close inspection of the bare cast part also has telltale signs of which half was done under pressure, although the minor resulting surface irregularities completely disappear under the first coat of primer...

关闭检查裸投下的一部分，也有迹象，其中一半是做压力下，虽然表面所造成的轻微违规行为完全消失下，第一层底漆。。。

In order to make use of time-frequency characteristic of signal,a new method of variational estimation on signal frequency by using AR model power spectrum estimation,designing band-pass filter to gain approximatively source signals and underdetermined mixed matrix,and constructing completed observation signals by expanded subspace primer vector is proposed.

充分利用信号的时频特性，提出了AR模型功率谱估计法滑动估计信号频率，设计带通滤波器近似获取源信号和欠定混合矩阵，以及扩展子空间向量基构造完备观测信号的方法，将问题转化为完备情况下的盲分离，最后运用FastICA方法实现了信号盲分离。

Myostatin gene sequence of Notheast Wapiti was cloned based on the closest species of cattle (Bos taurus cattle accession: AB076403) for the templates, with primer's design according to the conservative region of MSTN gene.

以物种最近的牛（Bos taurus cattle登陆号：AB076403）的Myostatin基因序列为模版，根据该基因保守序列，进行引物设计。

Primer 325 RAPD showed all COS-PA from SICU in 2003 were identical (SICU-A type), but the Burn-A type and SICU-A type were distinctly different. Most of the non-COS-PA from SICU also showed the trend of prevalence, which was defined as SICU-B type in RAPD typing, including the strain found in the bottom of the washstand.

SICU 2003年收集的COS-PA，经RAPD（引物325）分型为同一型别；在SICU收集的non-COS-PA也多数呈同一型别，自SICU水池下水口收集到的1株non-COS-PA也属于此型。

This method of primer design can be used to construct mutant plasmids with great ease and speed.

这种方法能用于超过20bp的碱基的插入或替代突变，及用于超过2000bp DNA片段的删除突变的研究。

Acephala By using SCR specific primers based on the NH_2-terminal region of SCR proteins and anchored oligo_(18) primer, SCR cDNA fragment was isolated from Brassica oleracea var.

然后，利用甘蓝SCR_(13)的5'非翻译区和3'非翻译区设计SCR_(13b)的特异性引物，获得了羽衣甘蓝SCR_(13b)编码区序列。

After thecomplete genome extraction of the strain was performed, the genomic DNA was partiallydigested by restriction enzyme Sau3AⅠ, the DNA fragments from 1 to 5Kb was clonedinto prokaryote expression vector pET-28a-c, and transformed host bacteria. The resultsshowed that we succeeded in constructing the gene expression library of haemophilusparasuis serovar 5, which is fundamental for the study of advanced gene screening. Inaddition, primer design was performed based on haemophilus influenzae in this study. In addition, PCR was performed by using genomic DNA of haemophilus parasuisserovar 5 as the template. The results demonstrated that we obtained two neo-gene:23SrRNA gene(conserved gene belonging to the large-subunit of ribosome) and adenylatecyclase gene(encodes adenylate cyclase and participates in converting adenyl nucleosidetriphosphate to cyclic adenosine3",5"-monophosphate). Furthermore, the phylogeneticanalyses between the species was performed, and neighbor-joining tree was constructedbased on comparison of 23S rRNA gene sequences, so it was illuminated betweenHaemophilus parasuis and other species in molecular evolution relationship.

选择我国流行优势菌株副猪嗜血杆菌血清5型地方株为研究对象，提取细菌基因组DNA，用限制性内切酶Sau3AⅠ对基因组DNA进行部分酶切，回收大小为1～5Kb的DNA片段，将其连接入原核表达载体pET-28a-c，最后转化宿主菌，结果成功地构建了基因表达文库，为后续的基因筛选工作奠定基础；另外，本研究选择嗜血杆菌属的流感嗜血杆菌为参考对象进行引物的设计，以副猪嗜血杆菌血清5型地方菌株的基因组DNA为模板，进行PCR扩增反应，结果表明成功地获得两个新基因：23S rRNA基因(存在于核糖体大亚基中的保守性基因)和腺苷酸环化酶基因(负责将腺嘌呤核苷三磷酸转变为环腺苷酸)，并进一步做了不同物种之间的分子系统发育分析，构建了基于23S rRNA基因的邻接法系统发育树，阐明了副猪嗜血杆菌与其它菌种的分子进化关系。

This paper expatiated and analyzed the progress and status in amplificative target and design of primer、range of sampling and assistant technique and the relative research fields of CHD gene.

阐述和分析了CHD基因在扩增目标及引物设计、取样范围及辅助技术和所涉足的科研领域等方面的发展及现状。

Anaphylactoid purpura ; children ; Hans ; HLA-DQA1 gene ; polymerase chain reaction-sequence specific primer

过敏性紫癜；儿童；汉族；人类白细胞抗原DQA1基因；聚合酶链反应-序列特异性引物

An optimized PCR reaction system for ISSR analysis was established:PCR was performed in a 20μl reaction mixture with 5μmol/L DNA, 2.5mmol/L Mg~(2+), 0.3mmol/Lof each dNTP, 300nmol/L primer, 1U Taq polymerase and 1×Buffer. Using 9 ISSRprimers,the genetic diversity among 23 Arrhenatherum elatius L.

3遗传多样性研究结果表明：通过模板DNA、引物、Mg~+、dNTPs、Taq酶五种ISSR反应体系的影响因素进行L_(16)(4~5)正交试验，建立了高燕麦草ISSR反应体系：20μl的反应体积中含有模板DNA 5μmol／L，引物300nmol／L，Mg~+2.5mmol／L，dNTP 0.3mmol／L，Taq酶1U及1×Buffer。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。