查询词典 primer

Hello proposed landlord which the Department of primer with light- colored shirt with a black look nice if a bit deep sense of lower body of the primer ...

你好建议楼主里面搭配浅色系的打底衫好看如果搭配黑色显得有点深沉的感觉下身的打底。。。

DNA were abstracted by means of phenol chloroform, amplification and cataphoresis test of polymerase chain reaction of target DNA,primer sequence of gene segment amplification of 5-hydroxytryptamine (5-HT) 2A receptor gene T102C: 5'-TCT GCT ACA AGT TCT GGC TT-3', 5 '-CTG CAG CTT TTT CTC TAG GG-3'; Reaction system of 50 μL, DNA 0.5 μg,Primer 50 pmol,TagDNA enzyme 2U were added with dNTP to the final concentration of 200 μmol/L.

应用常规苯酚氯仿法抽提DNA，目的DNA的聚合酶链反应扩增及电泳检测，5-羟色胺2A受体基因T102C基因片断扩增的引物序列：5'-TCT GCT ACA AGT TCT GGC TT-3'。5'-CTG CAG CTY TTT CTC TAGGG-3'；采用50 μL反应体系，DNA 0.5 μg，引物50 pmol，TagDNA酶2U加dNTP至终浓度200μmol/L。

The informative loci by each primer or primer pair were used to identify 10 cherry tomato varieties,respectively.

分析单引物的品种鉴别能力发现，RGA和SRAP标记具有较高的鉴别效率。

Fifty-three primer combinations were found to amplify nuclear DNA of Chinese cabbage, properly. They amplified the fragments up to 4 381 with an average of the 83. Among them, 9 markers derived from 9 primer combinations were linked to the clubroot resistance gene.

筛选出的适宜53对引物组合共扩增出4381条带，占总扩增片断的33.50%，平均每对扩增出83条，其中9个组合扩增出与抗根肿病基因连锁标记。

In this study the clubroot resistant CR DH line, susceptible inbred line 94SK of Chinese cabbage, the resistant and susceptible DNA bulks constructed from F2 individuals of CR DH line and 94SK were used to select the adaptive AFLP primer combination. AFLP analysis was performed with 256 Pst Ⅰ/Mse Ⅰ primer combinations.

为了筛选出大白菜适宜的AFLP引物组合，研究以抗根肿病大白菜双单倍体CR Shinki、感病自交系94SK及其后代F2构成的纯合抗病和纯合感病DNA混合池为材料，利用256对Pst Ⅰ/Mse Ⅰ引物组合进行了AFLP分析。

Three primer pairs located in psbA~trnK, psbB~psbH and trnR-ACG~trnN-GUU regions were applied in phylogenetic analysis according to Maximum parsimony and Neighbor-joining methods. The consistent results between the phylogenetic analysis and taxonomy indicated the application potential of the three primer pairs in phylogenetic analysis and species identification. However, due to no sequence variation observed in amplified regions between two vegetable soybean varieties (KS5 and KS8), two cowpea subspecies (Vigna unguiculata ssp. cylindrica and Vigna unguiculata ssp. sesquipedalis), and among three rice varieties (TNG67, TK9, and TCS10), the three primer pairs are not effective in identifying variation within species.

选择其中三组引子对(基因体位置为psbA~trnK、psbB~psbH及trnR-ACG~trnN-GUU)增幅片段之序列进行亲缘分析，以最大简约法及邻接法之分群结果皆与目前植物分类相符，故三组引子对在物种间亲缘关系分析及物种鉴定上都有不错的效果，位於psbA~trnK及psbB~psbH之引子对效果尤佳，但是三组引子对在物种内变异的辨识效果不甚理想，在两个毛豆品种间(高雄5号与高雄8号)、两个豇豆亚种间及三个水稻品种间(台农67号、台稉9号与台中秈10号)的增幅片段没有明显差异存在。

The typical spot symptoms on fruit would decrease the production and merchandise of tomato in the heavy rain of June and July; therefore, the key period for disease control is after rainy season. Quick identification on pathogen was using PCR. 1476bp band was amplified by primer P3、P4of bacterial speck. By using primer CMM5、CMM6 for bacterial canker, 614bp fragment was amplified. These PCR tests provided important foundation for pathogen identification and disease diagnoses. Using primer RST65 and RST69 for bacterial spot, expected 420bp band was not amplified.

通过聚合酶链式反应对病原进行快速鉴定，证明加工番茄细菌性斑点病用引物P3、P4可以得到一条大小为1476bp的特异性条带，番茄细菌性溃疡用CMM5、CMM6可以扩增出一条大小为614bp的特异性条带，这为病害的快速诊断打下了基础，但利用引物RST65和RST69对番茄细菌性疮痂病进行鉴定，没有得到预期420bp的目标条带，故对细菌性疮痂病的快速检测还有待继续研究。

Methods Using the well-conserved regions of the DNA polymerase gene in human herpesviruses,we synthesized two pairs of primer,including one pair of primer designed to amplify herpes simplex virus type 1 and 2,Epstein-Barr virus and cytomegalovirus,another pair of primer to varicella-zoster virus and human herpesvirus 6 by PCR.

2 0 0 2年 1～ 12月浙江大学儿童医院在疱疹类病毒高度同源序列DNA多聚酶基因中设计两对通用引物，该引物能同时扩增单纯疱疹病毒Ⅰ型与Ⅱ型、爱泼斯坦—马尔病毒、人巨细胞病毒、水痘带状疱疹病毒和人类疱疹病毒 6型(HHV6) 6种病毒。

The result would be used in tobacco breeding.4 SRAP technology is used to study the genetic differences among different types of tobacco, including flue-cured tobacco, oriental tobacco, maryland tobacco, burley tobacco, and air/sun cured tobacco, five pairs of primer combinations which can amplify characteristic bands has been screened. Among the five combinations, two of them (SF25*SR2 and SF3*SR26) can distinguish air-cured tobacco including maryland tobacco and Burley tobacco from the other cultivated forms; SF20*SR14 can distinguish sun cured tobacco including oriental tobacco and sun-cured tobacco; SF16*SR14 can distinguish flue-cured tobacco and air-cured tobacco, and SF22*SR14 can distinguish DBJ599 with specisl aroma and the other flue-cured tobacco.5 8 varieties , which represent the different types of tobacco, are divided into 3 groups. I: G-28, II:DBJ599、MD40、By21, III: Basma、Sumsun、XNS、QCT. The cluster analysis result es consistant with experience evatuation.6 Anorthogonal design was used to optimize a ILP-PCR system with 5 factors (DNA, Mg2+, dNTP, primer and Taq polymerase) at 4 levels.The result showed the optimized ILP-PCR system for Nicotiana tabacum L. was: 2uL10×PCRbuffer, 20ng template DNA, Mg2+ 2.0mmol/L, dNTP 150umol/L, primer 0.3umol/L, Taq DNA polymerase 1 U in a total of 20uL reactionsolution.

研究结果可作为烤烟与香料烟类型间的杂种鉴定利用的实验与理论依据。4、利用SRAP标记技术，研究和分析了烤烟、晒烟、晾烟、白肋烟、香料烟等不同栽培类型间的基因型差异，初步筛选出了能够在分子水平上反映不同烟草类型差异的特征性标记带：晾烟MD40、Ky17、Burley21、TN90基因型共有的特征带有两对引物组合，分别为SF25*SR2和SF3*SR26；晒烟小牛舌、青梗、小花青和香料烟Sumsun、komotini Basma、Xanthi Basma共有的特征带引物组合为SF20*SR14；NC89、K326、G-28、大白筋和MD40、Ky17、Burley21、TN90共有的特征带引物组合为SF16*SR14，烤烟中大白筋599的特征带引物组合SF22*SR14.5、利用61对SRAP引物组合，研究了普通烟草不同类型间的亲缘关系，将普通烟草类型间8个不同供试材料划分为3类别。I：烤烟类型G-28II：白肋烟类型(MD40、By21、大白筋599III：香料烟类型(Basma、Sumsun、小牛舌、千层塔)。

Subtractive hybridization was performed by annealing an excess of driver cDNA with each sample of adaptor ligated tester cDNA. The cDNAs were heat denatured and incubated in 68℃ for 8 hours.

杂交产物经过LA Taq DNA聚合酶补平末端后，以试剂盒提供的PCR Primer 1作为第一次PCR的引物，以Nested Primer 1和Nested Primer 2R作为第二次PCR的引物，通过抑制性PCR富集大量在Tester中高表达的差异表达基因的cDNA。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。