查询词典 primer

I put my railing on the sail and used it to scratch the primer where the rungs were.

我把我的栏杆上船帆，并用它从无到有，引如梯矢人。

Under normal climate, the anti-rust primer should immediately applied within 4 hours after sandblast finished ,otherwise the surface with rusted immediately in few hours.

在常规气候条件下，喷砂处理完的钢铁应在4小时内立即用相应防锈底漆涂饰。暴露在空气中的洁净钢铁表面，很快会在表面形成一薄层锈蚀。

The genetic relationship of 27 sapodilla cultivars and 4 mamey sapoae cultivars were analyzed by using fluorescent-labeled AFLP markers. A total of 1131 AFLP markers between 75 to 500 base pairs were generated with 9 primer combinations, of which 1096 (96.9%) were polymorphic.

利用荧光AFLP分子标记技术对27个人心果品种及4个蔓妹人心果品种资源的亲缘关系进行研究。9对引物共扩增得到1131条带(75~500 bp)，其中多态性带1096条(96.90%)。28个品种具特征性条带，特征带大小为350~500 bp。

Mitochondrial DNA extraction, long-PCR amplification and primer-walking sequencing methods were carried out for determining the complete mt DNA sequence of Scolopendra multilan.(1) The complete mitochondrial genome of Scolopendra multilan is 13,734 bp in size.

采用长片断扩增和PCR引物步移测序等方法，补齐少棘蜈蚣线粒体基因组全序列，主要结果如下：（1）序列全长为13，734 bp，其基因组成和顺序、基因的方向即编码链的选择、碱基G的低含量和对碱基A偏好以及GC和AT偏斜与已知的多足动物线粒体基因组相同。

Bulked segregant analysis revealed that 2 primer, phil 18 and phi 041, amplified polymorphic bands.

陈翠霞：南方玉米锈病的抗病性鉴定、抗病基因的遗传分析、分子标记和精细定位 4。

SSR techniques were employed to preliminary mapping of the resistance gene Rpp9-2. Bulked segregant analysis revealed that 2 primer, phi118 and phi041, amplified polymorphic bands.

对南方玉米锈病基因Rpp9-2进行SSR初步分析，表明，引物phi118和phi041在双亲和抗感基因池扩增出一致的多态条带。

Fertile and sterile pools were set up by bulked segregant analysis based on the selection for the highly fertile and highly sterile plants of Shanyou 63 F_2 populaiton The AFLP analysis of the two pools indicated that 64 primer combinations amplified 3 477 stable and clear bands.

选择汕优63F_2的高可育株和高不育株分别建立 2个基因池，利用 AFLP标记技术对2 池间的多态性进行了研究。分析表明，64组引物在两池间全部扩增出了稳定、清晰的带纹，共计3 477条带。

The results showed that RSAP used two primers of 18 nucleotides in which starting at the 3' end of each primer were restriction site sequences (4-6 bases),followed 12-14 bases of arbitary sequence, the differences between two primers were restriction sites and filler sequence; PCR am-plification was run for the first 5 cycles with an annealing temperature of 35℃, followed by 35 cycles with an annealing temperature of 48℃; the PCR reaction of 25 μL included 20ng DNA templates, 2.5mmol/L of Mg(superscript 2+), 0.2 mmol/L of dNTP, 1.5U of Taq DNA polymerase, 600nmol/L of each primers. RSAP is a new maker technique with simplicity, moderate throughput ratio and reliability. RSAP is of good reprodicibity and broad application.

结果显示，RSAP技术的引物为2条长度均为18bp的引物，引物的3'端为4~6个碱基的限制性酶切位点序列，接着是12~14个碱基的随机序列，2条引物的限制性位点和随机序列不同：PCR扩增的前5个循环采用35℃的退火温度，随后的35个循环采用48℃的退火温度；在25μL反应体系中，模板DNA用量为20ng，Mg(上标 2+)浓度为2.5mmol/L dNIPs浓度为0.2 mmol/L，Tap DNA聚合酶用碳为1.5U，2条引物浓度均为600mmol/L；RSAP技术重复性好，适用性广泛，是一种操作简便、产率中等、稳定可靠的DNA标记技术。

The RAPD was used to study the genetic diversity of 19 KinXin series potato cultivars. The result showed that 52 RAPD bands were amplified using 7 primer, among them 43 bands were polymorphic, and 4~9 bands were amplified in each lane. The RAPD analysis indicated that the genetic distance of 19 potato cultivars was between 0.17~0.72, average value was 0.39. When all of the polymorphic bands were analysed by cluster analysis, 19 potato cultivars were classified into 3 groups. The analysis showed that there existed close sibship among the majority of potato cultivars, and the genetic distance between the minority cultivars and the others is longer, which indicate that the genetic basis of KeXin series potato cultivars is wide.

利用RAPD标记技术对19份克新系列马铃薯品种进行了遗传多样性分析，分别提取19份马铃薯品种的DNA，进行随机引物多态性扩增，从1000条随机引物中初步筛选出7条有多态性的引物进行详细研究，每条RAPD引物扩增出4~9条带，共获得52条带，其中多态性条带为43条；19份马铃薯品种的遗传距离介于0.17~0.72之间，平均值为0.39，平均遗传距离介于0.31~0.51之间；聚类分析结果在GS=0.53处可将克新系列马铃薯品种划分为三类，聚类结果与系谱分析基本相符，同时也说明克新系列马铃薯的遗传基础有所拓宽。

If you're in such a state tonight, by file time you sit for the Palace Examination I dare say you'll even forget the first primer you ever read.

"宝钗见问，悄悄的咂嘴点头笑道："亏你今夜不过如此，将来金殿对策，你大约连'赵钱孙李'都忘了呢！""

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。