查询词典 primer

We have examined various conditions of the multiplex PCR, using a large number of primer pairs.

我们验证了各种条件下多重PCR的反应。

In order to compare the homology of the gene encoding VP2 between MDPV-Q and MDPV which was registered in the GenBank, also in order to find out changes of the VP2 gene and the immuno-genicity between the wild-strain MDPV-Q and the attenuated strain MDPV-26 which derived by continued passage of virulent wild-type in muscovy duck embryo, a pair of primer (LHMP7/LHMP8) was designed. The upper one LHMP7 and the lower one LHMP8 corresponded to the MDPV-specific nucleotides sequence 2885-2900 and 4618-4604 respectively, according to the MDPV nucleotide sequence databases. The length of the sequence embraced by the primers was 1734bp.

为了获取这一基因片段与国外分离株进行同源性比较，同时也为了了解MDPV强、弱毒株VP2基因之间的异同关系，及其免疫原性的变化规律，通过DNA重组技术，设计了一对引物LHMP7／LHMP8，该对引物选取位于2885～2900及4618～4604的两段序列，跨幅为1734bp，并在这两条引物中分别加入两种限制性核酸内切酶SacⅡ和KpnⅠ的酶切位点，分别对MDPV-Q和由该株病毒经人工致弱后得到的MDPV-26株进行PCR扩增，并将PCR产物克隆到pMD18-T载体上，分别得到二个重组子：pMD18-T—M-Q VP2和pMD18-T-M-26 VP2。

Comparison experiments with corresponding random primer pairs are conducted to identify the nonobjective products.

实验中设置相应随机引物的RAPD对照，识别非目的扩增产物。文中介绍了新方法的原理，分析了这种方法的优缺点。

Methods Real-time PCR, using the primer-probe specific for the yst gene, was applied to detect the pathogenic （7 serotypes） and nonpathogenic （ 5 serotypes） Yersinia enterocolitica, other species of Yersinia （8 types）, and other enteric bacteria （8 types）.

应用针对致病性小肠结肠炎耶尔森菌染色体上yst基因而设计的特异引物和探针进行实时定量聚合酶链反应，检测致病性（7种血清型）和非致病性（5种）小肠结肠炎耶尔森菌、耶尔森菌属内的其他菌种（8种）及其他肠道细菌（8种）。

However, because primers were nonsignificant factors, which was presented by their small R value, we took A level of primer as 0.25 μmol/L.

但由于引物作为影响因素的R值小，是非显著因素，因此引物取A水平即0.25 μmol/L。

MicroRNAs primers were arrayed on plates according to the Tm of each primer. PCR were carried out at different annealing temperatures using a gradient real-time PCR instrument. The relative expression level of each microRNAs was calculated using the most stable reference gene as normalizer which was selected by geNorm and Normfinder software.

通过geNorm软件和Normfinder软件分析选择最佳的内参照基因，将每种microRNAs按其引物Tm值排成阵列，用梯度实时PCR仪在不同的退火温度扩增，并计算每种相对于microRNAs内参照基因的表达量。

Based on twin primer design, we developed a general real-time method to trace nuclease activity.

在孪生引物的基础上设计出荧光淬火双链探针，建立了研究DNase Ⅰ酶切活性的实时示踪方法。

Increasingly the curriculum in many public schools is becoming a primer in occultism.

在许多公立学校的课程日益成为引占卜。

1 Oven order 5.1.1 first hearth cloth Wind board laying 200mm thickness of the bed material; About Revert cloth laying Wind board thickness 200mm bed material, to prevent Ogive burn plug secondary air pipe; 5.1.2 prior to Add a small amount of flammable wood furnace and left and right back Feeder, ignition Wind Road in oven two days before ignition; 5.1.3 ignition started Ovens need to use a small amount of diesel added to the accumulation of wood, wood ignition when combustible, wood drying to around 200 ° C, according to adjust primer wind baffle and then curves of thermal insulation and heating.

5.1烘炉顺序 5.1.1先将炉床布风板上铺设厚度为200mm的床料；左右返料器布风板上铺设厚度200mm床料，防止把风帽烧坏，堵塞二次风管； 5.1.2事先将少量易燃木柴放入炉膛和左、右返料器，点火风道应在烘炉前2天进行点火； 5.1.3开始烘炉点火时需要用少量的柴油加在堆积的木柴上，点火时木柴易燃烧，木柴烘至200℃左右后，根据情况调节引风挡板，再按升温曲线保温、升温。

Coconut SSR primer pairs were used to amplify 39 different genera of palm family, the effective amplification is 90.5%, and the polymorphism level among the speciese of these genera was relatively high..

供试的42份国内外椰子种质材料的遗传相似系数范围大部分在0.6~0.8之间，最小为0.503，最大为0.961。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。