查询词典 primer

We have examined various conditions of the multiplex PCR, using a large number of primer pairs.

我们验证了各种条件下多重PCR的反应。

In order to compare the homology of the gene encoding VP2 between MDPV-Q and MDPV which was registered in the GenBank, also in order to find out changes of the VP2 gene and the immuno-genicity between the wild-strain MDPV-Q and the attenuated strain MDPV-26 which derived by continued passage of virulent wild-type in muscovy duck embryo, a pair of primer (LHMP7/LHMP8) was designed. The upper one LHMP7 and the lower one LHMP8 corresponded to the MDPV-specific nucleotides sequence 2885-2900 and 4618-4604 respectively, according to the MDPV nucleotide sequence databases. The length of the sequence embraced by the primers was 1734bp.

为了获取这一基因片段与国外分离株进行同源性比较，同时也为了了解MDPV强、弱毒株VP2基因之间的异同关系，及其免疫原性的变化规律，通过DNA重组技术，设计了一对引物LHMP7／LHMP8，该对引物选取位于2885～2900及4618～4604的两段序列，跨幅为1734bp，并在这两条引物中分别加入两种限制性核酸内切酶SacⅡ和KpnⅠ的酶切位点，分别对MDPV-Q和由该株病毒经人工致弱后得到的MDPV-26株进行PCR扩增，并将PCR产物克隆到pMD18-T载体上，分别得到二个重组子：pMD18-T—M-Q VP2和pMD18-T-M-26 VP2。

Comparison experiments with corresponding random primer pairs are conducted to identify the nonobjective products.

实验中设置相应随机引物的RAPD对照，识别非目的扩增产物。文中介绍了新方法的原理，分析了这种方法的优缺点。

Methods Real-time PCR, using the primer-probe specific for the yst gene, was applied to detect the pathogenic （7 serotypes） and nonpathogenic （ 5 serotypes） Yersinia enterocolitica, other species of Yersinia （8 types）, and other enteric bacteria （8 types）.

应用针对致病性小肠结肠炎耶尔森菌染色体上yst基因而设计的特异引物和探针进行实时定量聚合酶链反应，检测致病性（7种血清型）和非致病性（5种）小肠结肠炎耶尔森菌、耶尔森菌属内的其他菌种（8种）及其他肠道细菌（8种）。

However, because primers were nonsignificant factors, which was presented by their small R value, we took A level of primer as 0.25 μmol/L.

但由于引物作为影响因素的R值小，是非显著因素，因此引物取A水平即0.25 μmol/L。

MicroRNAs primers were arrayed on plates according to the Tm of each primer. PCR were carried out at different annealing temperatures using a gradient real-time PCR instrument. The relative expression level of each microRNAs was calculated using the most stable reference gene as normalizer which was selected by geNorm and Normfinder software.

通过geNorm软件和Normfinder软件分析选择最佳的内参照基因，将每种microRNAs按其引物Tm值排成阵列，用梯度实时PCR仪在不同的退火温度扩增，并计算每种相对于microRNAs内参照基因的表达量。

Based on twin primer design, we developed a general real-time method to trace nuclease activity.

在孪生引物的基础上设计出荧光淬火双链探针，建立了研究DNase Ⅰ酶切活性的实时示踪方法。

Increasingly the curriculum in many public schools is becoming a primer in occultism.

在许多公立学校的课程日益成为引占卜。

1 Oven order 5.1.1 first hearth cloth Wind board laying 200mm thickness of the bed material; About Revert cloth laying Wind board thickness 200mm bed material, to prevent Ogive burn plug secondary air pipe; 5.1.2 prior to Add a small amount of flammable wood furnace and left and right back Feeder, ignition Wind Road in oven two days before ignition; 5.1.3 ignition started Ovens need to use a small amount of diesel added to the accumulation of wood, wood ignition when combustible, wood drying to around 200 ° C, according to adjust primer wind baffle and then curves of thermal insulation and heating.

5.1烘炉顺序 5.1.1先将炉床布风板上铺设厚度为200mm的床料；左右返料器布风板上铺设厚度200mm床料，防止把风帽烧坏，堵塞二次风管； 5.1.2事先将少量易燃木柴放入炉膛和左、右返料器，点火风道应在烘炉前2天进行点火； 5.1.3开始烘炉点火时需要用少量的柴油加在堆积的木柴上，点火时木柴易燃烧，木柴烘至200℃左右后，根据情况调节引风挡板，再按升温曲线保温、升温。

Coconut SSR primer pairs were used to amplify 39 different genera of palm family, the effective amplification is 90.5%, and the polymorphism level among the speciese of these genera was relatively high..

供试的42份国内外椰子种质材料的遗传相似系数范围大部分在0.6~0.8之间，最小为0.503，最大为0.961。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。