查询词典 primer

Once there are a few bases built in, the ionic bond is so strong between the template and the primer, that it does not break anymore.

一旦有几个碱基掺入，此时在引物与模板之间的离子肩更强，不会再断裂。

Single PCR reaction with two pairs of PCR primers designed on the markers respectively tightly linked with Cf-9 and Tm-1 genes in tomato, which be resistant to leaf mold and tomato mosaic virus. For the genes the PCR products were almost completely correspond to the amplified bands produced by single PCR primer.

利用同一PCR反应体系，对分别与番茄抗叶霉病的Cf-9基因和抗番茄烟草花叶病毒病的Tm-1基因紧密连锁的PCR标记进行了同时扩增筛选，扩增的特异性片段与单引物扩增片段吻合。

ITS sequences of ten kinds of 10s plants of Lespedeza and an outgroup were obtained by primer design PCR,sequencing and cluster analysis.

用PCR产物直接测序法对10种胡枝子属植物和1个外类群的ITS序列进行了测定。

I borrowed a primer of science from the library.

我从图书馆借了一本科学的入门书。

Analysis of the obtained genome amplification showed that DNA after PCR presented obvious polymorphism, and the results were the same using the same primer to amplify the larva and imago of the same species. It indicates that RAPD technique can be applied to the identification of dry samples and dipped larva sample of longicorn beetles as well as the research of phylogeny. These three methods for extracting genome DNA from different species of longicorn beetles at different preserved time are feasible.

对所获得的基因组扩增分析表明，经过PCR之后的DNA能呈现明显的多态性，同种天牛用同一引物扩增幼虫和成虫所获得的结果相同，可以认为，RAPD技术可以应用于天牛成虫干标本及幼虫浸渍标本的鉴定及系统发育研究中，也证明了这3种方法用于提取不同保存时间、不同种天牛基因组的DNA是切实可行的。

In this study, a novel EoRab gene was cloned from Euplotes octocarinatus by degenerated primer polymerase chain reaction, with a length of 814 bp in macronucleus and 669 bp in the coding region.

本研究运用PCR技术从原生动物八肋游仆虫大核基因组中克隆获得了一个新的EoRab基因，该基因在八肋游仆虫大核基因组中全长814bp，编码区为669bp，拟编码222个氨基酸。

Different color shop primer to be used for mild and high tensile steel plates and sections.

不同颜色的车间底漆用于温和、高强度钢板，部分。

No fragments were amplified from some loci of EST-SSRs, which may suggest the loss of these loci in mirror carp genome or sequence divergence at the primer binding sites. These null alleles may result from selection because functional genes are under more selection pressure than non-encoding loci. Overall, population genetic variation is high for each of the five mirror carp, and the differentiations are also significant among populations.

3在与功能基因相关的多个微卫星基因座位上，扩增产物呈现不同程度的缺失现象，这些无效等位基因的产生可能与结构基因在育种中受到人工选择的影响较大有关。

A 25μL solution with genomic DNA 25ng/μL, MgCl_2 1.5mmol/L, dNTPs 0.1mmol/L, random primer 0.1μmol/L, 1U TaqDNA polymerase would be optimum for the amplification reaction. A high reproducibility was obtained with the optimized experiment conditions. The results of the genetic diversity among M. Mongolic, M. macrocopa and their s...

按照优化的RAPD条件进行实验，重现性良好。2种裸腹在盐胁迫条件下的种群遗传多样性分析表明，同种裸腹不同生存盐度之间的种群遗传相似度较高，分别为0.9134和0.9038；而多刺裸腹和蒙古裸腹两个种间的遗传相似度相对较低，约为0.5～0.6。

Keywords : monocotyledonous crop;dicotyledonous crop;EST-SSR;transferability of primer

中文关键词：单子叶作物；双子叶作物；EST-SSR；引物通用性

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。