查询词典 primer-detonator

At that time, I have learn the nobleness of lotus flowers which are not polluted absolutely growing in mud… When at the age I could speak, my grandfather returned from work and taught me to intonate the "Three Character Primer" and Tang poem.

我会说话时，外公下班回来教我朗诵唐诗宋诗和三字经。

On the other hand,the Inverse PCR combined with a tail-to-tail primer design requires T4 DNA ligase enzyme to ligate PCR products,which makes the positive rate relatively low.We aim to find out an easy and effective method to perform gene deletion, insertion and substitution mutation.

近几年，有文献报道使用基于Quikchange原理的方法来制作DNA片段的插入、替代以及删除突变，但由于QCM-PCR的低产量，难以产生阳性克隆。

Methods HLA-A locus was typed with polymerase chain reaction-sequence specific primer technique for 259 longevous people (aged 90～105) and 214 healthy,randomly sampled subjects (aged 23～70) who live in the same area and comparative analyses were carried out among relevant alleles between the two groups.

方法应用聚合酶链式反应-序列特异引物对广西巴马和东兰两县259名90岁以上壮族长寿老人(90~105岁)和214名当地健康、随机抽样的壮族成年人(23~70岁)进行HLA-A座位的基因分型并进行相应等位基因频率比较。

The results showed that the optimized content of Mg~(2+) was 5mmol/L; dNTP was 200μmol/L; Primer was 0.5μmol/L; Taq polymerase was lU/50ul in the PCR system. Only one fragment of about 270bp was amplified within the genome of 12 wild species and 23 cultivars of Mallus Mill., which indicated that Ty1-copia-like retrotransposons were archaic components of apple genome. The nested insertion of near and within the reverse transcriptase gene of Ty1-copia-like retrotransposons wasn\'t found in apple genome.2 The reverse transcriptase conservative region of Ty1-copia-like retrotransposons were amplified from Gala apple using the optimized PCR system. The amplification product was isolated, cloned and sequenced. Forty-five clones containing reverse transcriptase conservative region were obtained (accession number: AY849580 -AY849592 and DQ105035-DQ105066). Cluster analysis of these sequences showed a great heterogeneity among RT domains isolated from the same genotype.

从苹果属12个野生种和苹果23个栽培品种中都扩增到了约270bp的目的产物，且所有试材的扩增结果一致，均无目的条带以外的产物扩增，说明Ty1-copia类逆转座子在苹果属植物中广泛存在，且存在历史可能较为久远；从扩增结果看不出该逆转座子的逆转录酶基因或附近区域在苹果基因组内有嵌套行为的发生。2、利用优化了的PCR方法从嘎拉苹果基因组克隆了45条Ty1-copia类逆转座子逆转录酶保守序列(登陆号分别为AY849580-AY849592和DQ105035-DQ105066)，结果表明，同一基因组来源的这些序列存在高度的异质性。

This article serves as a primer for people interested in gaining a better understanding of matrices.

这篇文章是让感兴趣的人一个对矩阵有一个好一点的初步理解。

In design of formulation of S97 salt-spray and corrosion resistant coatings, AC impedance electrochemical technology is used for determination of pigment volume concentration of epoxy micaceous oxide primer and acrylic polyurethane top coat.

在S97耐盐雾防腐蚀涂料的配方设计阶段，采用电化学交流阻抗技术确定了环氧云铁防锈底漆和丙烯酸聚氨酯面漆的颜料体积浓度，并对S97耐盐雾防腐蚀涂料的防腐蚀性能进行了研究。

The performance of the selected coating systems was experimentally verified,while some of the selected costing systems were brought into engineering practice.As the results,it was suggested to use a coating system composed of zinc-rich epoxy primer,micaceous ferric oxide epoxy intermediate,and modifie...

结果显示，对完全浸泡的设备，采用环氧富锌底漆+环氧云铁中间漆+改性环氧面漆的涂层体系有很好的防护效果；对干湿交替的设备采用环氧富锌底漆+环氧云铁中间漆+丙烯酸聚氨酯面漆的防护体系具有良好的防护性能，预计其防护寿命可达到8a以上。

Secondly the product of the DOP-PCR of Y1,Y2,X+4,1 chromosome of Muntiacus crinifrons were used as the templates of the next amplification using the special primer devised according to the human SRY gene .

再以黑麂的Y1，Y2，X+4和1号染色体的DOP-PCR产物为模板，用人的特异性的SRY（sex determining region of the Y chromosome）基因引物对其进行扩增，结果表明黑麂只有Y2染色体出现了SRY扩增片段。

This study consists 3 partsPart I: the research on the mechanism of affecting the growth of CAM vesselsBecause neoformative vessels is most related with bFGF and VEGF, this study designed bFGF, VEGF and the primer of its receptor, by the experiment of the generation of CAM vessels, draw the materials from CAM tissues consist account of vessels, by the experiment of RT-PCR, reserve the influence of HSYA on the expression of bFGF, VEGF, VEGF-R(flt-1) in CAM tissues.

本实验分为三部分：第一部分，HSYA影响鸡胚尿囊膜血管生成的机理研究由于新生血管与bFGF、VEGF及其受体的关系最为密切，本实验设计了bFGF、VEGF及其受体的引物，利用CAM血管生成实验，直接从富含血管的CAM组织上取材，通过RT-PCR实验，观察HSYA对CAM组织中上述三个基因表达的影响。

Based on the genomic DNA extracted from Ochotona curzoniae, the effects of the main reaction system elements such as Mg(superscript 2+), dNTPs, primer, template DNA, Taq DNA polymerase and annealing temperature were presented by single factor experiment.

方法］以高原鼠兔基因组DNA为模板，通过单因素试验，对体系中的模板浓度、Mg(上标 2+)浓度、dNTPs浓度、Taq酶用量、引物用量、退火温度进行探讨。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。